Restriction map for
pFN21AE1676
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfaI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FokI
FseI FspI HaeII Hin1I HindIII
HpaI HphI KasI KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 77 GGTACC G^GTACC
AfaI 78 GTAC GT^AC
AflII 175 CTTAAG C^TTAAG
AlwI 254 GGATC GGATC(4/5)
Asp718I 77 GGTACC G^GTACC
AspI 519 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 133 CCCGAG C^[CT]CG[GA]G
BbsI 516 GAAGAC GAAGAC(2/6)
BclI 554 TGATCA T^GATCA
BcnI 82 CCCGG CC^[GC]GG
BfrI 175 CTTAAG C^TTAAG
BpuAI 516 GAAGAC GAAGAC(2/6)
BsmAI 387 GTCTC GTCTC(1/5)
BsoBI 133 CCCGAG C^[CT]CG[GA]G
BspHI 326 TCATGA T^CATGA
BsrBI 36 GAGCGG CCGCTC(-3/-3)
BstNI 60 CCAGG CC^[AT]GG
CfoI 52 GCGC GCG^C
DraII 359 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 72 CCACGG C^C[GA][CT]GG
EarI 294 GAAGAG CTCTTC(1/4)
EcoO109I 359 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 60 CCAGG ^CC[AT]GG
FbaI 554 TGATCA T^GATCA
HapII 83 CCGG C^CGG
HhaI 52 GCGC GCG^C
HinP1I 52 GCGC G^CGC
HincII 502 GTCAAC GT[CT]^[GA]AC
HindII 502 GTCAAC GT[CT]^[GA]AC
HpaII 83 CCGG C^CGG
KpnI 77 GGTACC GGTAC^C
Ksp632I 294 GAAGAG CTCTTC(1/4)
MseI 176 TTAA T^TAA
MspI 83 CCGG C^CGG
MvaI 60 CCAGG CC^[AT]GG
NciI 82 CCCGG CC^[GC]GG
PflFI 519 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 284 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 191 CTGCAG CTGCA^G
PvuII 211 CAGCTG CAG^CTG
RcaI 326 TCATGA T^CATGA
RsaI 78 GTAC GT^AC
SapI 294 GAAGAGC GCTCTTC(1/4)
SfaNI 86 GCATC GCATC(5/9)
SfcI 191 CTGCAG C^T[GA][CT]AG
Tru9I 176 TTAA T^TAA
TspRI 551 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 519 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 284 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 428 CCATTATAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanI 77 GGTACC G^G[CT][GA]CC
126 GGTGCC
BpmI 214 CTGGAG CTGGAG(16/14)
369 CTGGAG
BseRI 94 GAGGAG GAGGAG(10/8)
136 GAGGAG
BsmI 48 GAATGC GAATGC(1/-1)
220 GCATTC
BssKI 60 CCAGG ^CC[ACGT]GG
82 CCCGG
Cfr13I 57 GGCCC G^G[ACGT]CC
360 GGGCC
DpnII 255 GATC ^GATC
555 GATC
HaeIII 57 GGCC GG^CC
361 GGCC
HgaI 519 GACGC GACGC(5/10)
187 GCGTC
HinfI 171 GATTC G^A[ACGT]TC
463 GAATC
MboI 255 GATC ^GATC
555 GATC
MspA1I 211 CAGCTG C[AC]G^C[GT]G
339 CCGCTG
MwoI 45 GCTGAATGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
341 GCTGGAATAGC
NdeII 255 GATC ^GATC
555 GATC
NlaIII 198 CATG CATG^
327 CATG
Sau3AI 255 GATC ^GATC
555 GATC
Sau96I 57 GGCCC G^G[ACGT]CC
360 GGGCC
ScrFI 60 CCAGG CC^[ACGT]GG
82 CCCGG
SmlI 16 CTTGAG C^T[CT][GA]AG
175 CTTAAG
TfiI 171 GATTC G^A[AT]TC
463 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsiYI BslI Cac8I DdeI
FauI Fnu4HI ItaI MboII MnlI
NlaIV TseI Tsp509I