################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BspEI BspHI BspMI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 128 GACGCC G[GA]^CG[CT]C AflIII 483 ACATGT A^C[GA][CT]GT AlwI 600 GGATC GGATC(4/5) AspHI 358 GTGCTC G[AT]GC[AT]^C AvaI 457 CCCGAG C^[CT]CG[GA]G BanII 254 GAGCCC G[GA]GC[CT]^C BbvCI 100 GCTGAGG CCTCAGC(-5/-2) BsaHI 128 GACGCC G[GA]^CG[CT]C BseRI 421 CTCCTC GAGGAG(10/8) BsiHKAI 358 GTGCTC G[AT]GC[AT]^C BsmAI 300 GAGAC GTCTC(1/5) BsmI 525 GAATGC GAATGC(1/-1) BsoBI 457 CCCGAG C^[CT]CG[GA]G Bsp1407I 441 TGTACA T^GTACA BspDI 88 ATCGAT AT^CGAT BspLU11I 483 ACATGT A^CATGT BsrFI 407 GCCGGC [GA]^CCGG[CT] BsrGI 441 TGTACA T^GTACA BssSI 133 CTCGTG CACGAG(-5/-1) BstAPI 615 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 453 CATCC GGATG(2/0) BstYI 600 GGATCT [GA]^GATC[CT] Cac8I 407 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 407 GCCGGC [GA]^CCGG[CT] ClaI 88 ATCGAT AT^CGAT DpnII 601 GATC ^GATC DraII 320 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 322 GACCCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 409 CGGCCA [CT]^GGCC[GA] EarI 106 GAAGAG CTCTTC(1/4) EcoNI 426 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 320 AGGACCC [GA]G^G[ACGT]CC[CT] FokI 453 CATCC GGATG(9/13) HapII 408 CCGG C^CGG HgaI 128 GACGC GACGC(5/10) Hin1I 128 GACGCC G[GA]^CG[CT]C HpaII 408 CCGG C^CGG Ksp632I 106 GAAGAG CTCTTC(1/4) MboI 601 GATC ^GATC MflI 600 GGATCT [GA]^GATC[CT] MspA1I 530 CAGCTG C[AC]G^C[GT]G MspI 408 CCGG C^CGG NaeI 407 GCCGGC GCC^GGC NdeII 601 GATC ^GATC NgoMIV 407 GCCGGC G^CCGGC NspI 483 ACATGT [GA]CATG^[CT] PleI 302 GACTC GAGTC(4/5) PpuMI 320 AGGACCC [GA]G^G[AT]CC[CT] PvuII 530 CAGCTG CAG^CTG Sau3AI 601 GATC ^GATC SfcI 549 CTACAG C^T[GA][CT]AG SspBI 441 TGTACA T^GTACA TfiI 228 GAATC G^A[AT]TC Tsp509I 471 AATT ^AATT XhoII 600 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 331 GTCTAC GT^[AC][GT]AC 547 GTCTAC AluI 296 AGCT AG^CT 531 AGCT AlwNI 337 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 364 CAGGGTCTG AvaII 321 GGACC G^G[AT]CC 492 GGTCC BmyI 254 GAGCCC G[AGT]GC[ACT]^C 358 GTGCTC BpmI 361 CTCCAG CTGGAG(16/14) 533 CTGGAG Bsp1286I 254 GAGCCC G[AGT]GC[ACT]^C 358 GTGCTC BsrI 354 ACTGG ACTGG(1/-1) 597 ACTGG EcoT14I 95 CCAAGG C^C[AT][AT]GG 584 CCTTGG HinfI 228 GAATC G^A[ACGT]TC 302 GACTC MseI 35 TTAA T^TAA 113 TTAA NlaIV 321 GGACCC GG[ACGT]^[ACGT]CC 384 GGAACC SfaNI 91 GATGC GCATC(5/9) 621 GATGC StyI 95 CCAAGG C^C[AT][AT]GG 584 CCTTGG TaqI 17 TCGA T^CGA 89 TCGA Tru9I 35 TTAA T^TAA 113 TTAA TspRI 285 CAGTG CA[GC]TG[ACGT][ACGT]^ 552 CAGTG TthHB8I 17 TCGA T^CGA 89 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AfaI BbvI BsaJI BsiYI BslI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI MwoI NlaIII RsaI Sau96I ScrFI TseI