(Circular) (Six-base) MAPSORT of: pFN21AE6255.seq Check: 132 from: 1 to: 5383 pFN21AE6255 5383 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3193 278 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2337 2553 2726 1937 Size: 400 216 173 4594 Fragments arranged by size: 4594 400 216 173 Acc65I G'GTAC_C Cuts at: 2704 2704 Size: 5383 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 3446 4830 5098 1632 Size: 545 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 545 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 2488 4282 5259 1203 Size: 1285 1794 977 1327 Fragments arranged by size: 1794 1327 1285 977 AgeI A'CCGG_T Cuts at: 4214 4214 Size: 5383 AhdI GACnn_n'nnGTC Cuts at: 4112 4112 Size: 5383 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 2347 2374 3126 4698 2347 Size: 27 752 1572 3032 Fragments arranged by size: 3032 1572 752 27 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2692 2918 3070 5179 1493 Size: 1199 226 152 2109 1697 Fragments arranged by size: 2109 1697 1199 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2462 2708 1870 Size: 73 519 246 4545 Fragments arranged by size: 4545 519 246 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 4159 618 Size: 325 217 627 917 1455 1842 Fragments arranged by size: 1842 1455 917 627 325 217 BanII G_rGCy'C Cuts at: 1391 2263 2702 1391 Size: 872 439 4072 Fragments arranged by size: 4072 872 439 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4412 BbvCI CC'TCA_GC Cuts at: 2106 2106 Size: 5383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3574 3608 1903 Size: 34 1637 34 3678 Fragments arranged by size: 3678 1637 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3994 136 Size: 107 122 71 631 605 2322 1525 Fragments arranged by size: 2322 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5378 5378 Size: 5383 BlpI GC'TnA_GC Cuts at: 2800 2800 Size: 5383 Bme1580I G_kGCm'C Cuts at: 1790 3444 4600 1790 Size: 1654 1156 2573 Fragments arranged by size: 2573 1654 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3114 4072 442 Size: 416 53 83 399 24 1697 958 1753 Fragments arranged by size: 1753 1697 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2559 4043 1239 Size: 1112 208 1484 2579 Fragments arranged by size: 2579 1484 1112 208 Bpu10I CC'TnA_GC Cuts at: 2106 2106 Size: 5383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 4373 4671 3508 Size: 865 298 4220 Fragments arranged by size: 4220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4046 882 Size: 617 2547 2219 Fragments arranged by size: 2547 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 5377 5377 Size: 5383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 3190 3572 4207 Size: 53 83 186 936 601 1056 382 635 Cuts at: 4207 275 Size: 1451 Fragments arranged by size: 1451 1056 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3816 4214 4488 4635 5046 3816 Size: 398 274 147 411 4153 Fragments arranged by size: 4153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2349 2379 2409 2439 1178 Size: 30 165 30 946 30 30 30 4122 Fragments arranged by size: 4122 946 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 1186 Size: 1231 4152 BseYI C'CCAG_C Cuts at: 4586 5082 4586 Size: 496 4887 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4810 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 3594 4622 1540 Size: 275 186 832 761 1028 2301 Fragments arranged by size: 2301 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 3444 3529 4600 2367 Size: 1077 85 1071 3150 Fragments arranged by size: 3150 1077 1071 85 BsmI GAATG_Cn' Cuts at: 2536 2894 2987 2536 Size: 358 93 4932 Fragments arranged by size: 4932 358 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2367 2702 3444 3529 4600 1391 Size: 399 473 104 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 335 104 85 BspEI T'CCGG_A Cuts at: 5046 5046 Size: 5383 BspHI T'CATG_A Cuts at: 3272 5002 3272 Size: 1730 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 844 Size: 1894 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3270 1394 Size: 429 1447 3507 Fragments arranged by size: 3507 1447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3878 4052 65 Size: 1183 2630 174 1396 Fragments arranged by size: 2630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2412 4027 4214 1700 Size: 110 602 1615 187 2869 Fragments arranged by size: 2869 1615 602 187 110 BsrGI T'GTAC_A Cuts at: 96 2446 96 Size: 2350 3033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5383 BssSI C'ACGA_G Cuts at: 2138 3443 4455 2138 Size: 1305 1012 3066 Fragments arranged by size: 3066 1305 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 3551 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstYI r'GATC_y Cuts at: 1972 2605 2713 3080 3465 3482 4934 5020 5032 Size: 633 108 367 385 17 1452 86 12 Cuts at: 5032 5378 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 633 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4644 Fragments arranged by size: 4644 683 56 BtsI GCAGTG_nn' Cuts at: 2899 3693 3713 2899 Size: 794 20 4569 Fragments arranged by size: 4569 794 20 ClaI AT'CG_AT Cuts at: 1554 2094 1554 Size: 540 4843 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2333 4390 809 Size: 507 1017 2057 1802 Fragments arranged by size: 2057 1802 1017 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2414 2830 3719 Size: 54 1425 50 275 18 584 416 889 Cuts at: 3719 5348 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 584 416 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2830 1537 Size: 275 1018 4090 Fragments arranged by size: 4090 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3313 1353 Size: 753 1207 3423 Fragments arranged by size: 3423 1207 753 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3970 4344 4490 387 Size: 1064 138 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2351 2559 3446 4043 4830 5098 Size: 393 545 174 208 887 597 787 268 Cuts at: 5098 1239 Size: 1524 Fragments arranged by size: 1524 887 787 597 545 393 268 208 174 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2435 Size: 5383 EcoO109I rG'GnC_Cy Cuts at: 2326 2326 Size: 5383 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 4530 1678 Size: 2852 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2592 2624 4024 Size: 32 135 32 689 32 299 32 1400 Cuts at: 4024 4056 4098 4130 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1400 689 299 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2727 2973 669 Size: 988 281 789 246 3079 Fragments arranged by size: 3079 988 789 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5383 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KpnI G_GTAC'C Cuts at: 2708 2708 Size: 5383 MfeI C'AATT_G Cuts at: 2982 2982 Size: 5383 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2404 4497 4681 1305 Size: 1099 2093 184 2007 Fragments arranged by size: 2093 2007 1099 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3561 Fragments arranged by size: 3561 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3341 3700 3859 518 Size: 648 2175 359 159 2042 Fragments arranged by size: 2175 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2537 2799 3476 4624 4869 1606 Size: 931 262 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 262 245 NaeI GCC'GGC Cuts at: 2414 2414 Size: 5383 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5383 NgoMIV G'CCGG_C Cuts at: 2412 2412 Size: 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2492 2741 4286 1132 Size: 97 1263 249 1545 2229 Fragments arranged by size: 2229 1545 1263 249 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5383 PciI A'CATG_T Cuts at: 2488 4282 2488 Size: 1794 3589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5383 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2326 2326 Size: 5383 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PstI C_TGCA'G Cuts at: 830 2735 830 Size: 1905 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 PvuII CAG'CTG Cuts at: 2537 2537 Size: 5383 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SbfI CC_TGCA'GG Cuts at: 2735 2735 Size: 5383 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SfcI C'TryA_G Cuts at: 826 1045 2554 2731 3866 4547 4738 826 Size: 219 1509 177 1135 681 191 1471 Fragments arranged by size: 1509 1471 1135 681 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5383 SmaI CCC'GGG Cuts at: 1872 2710 1872 Size: 838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 4388 4650 820 Size: 197 926 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 3307 5 Size: 47 3255 2081 Fragments arranged by size: 3255 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5383 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2589 2822 513 Size: 552 939 96 489 233 3074 Fragments arranged by size: 3074 939 552 489 233 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 2466 3629 Size: 274 80 33 51 1443 469 20 1163 Cuts at: 3629 5341 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1163 469 274 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 2409 2441 5343 5375 2124 Size: 32 253 32 2902 32 2132 Fragments arranged by size: 2902 2132 253 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5383 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2708 1870 Size: 838 4545 XmnI GAAnn'nnTTC Cuts at: 3512 3512 Size: 5383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3191 276 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI