################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AflIII AgeI AocI Aor51HI ApaI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EarI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KpnI Ksp632I MfeI MluI MluNI MroI MscI MunI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AclI 950 AACGTT AA^CGTT AcyI 49 GGCGCC G[GA]^CG[CT]C AhdI 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1013 GTGCAC G^TGCAC AlwI 513 GATCC GGATC(4/5) ApaLI 1013 GTGCAC G^TGCAC AscI 417 GGCGCGCC GG^CGCGCC Asp700I 81 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 49 GGCGCC G^G[CT][GA]CC BbeI 49 GGCGCC GGCGC^C BciVI 1137 GTATCC GTATCC(6/5) BglII 1264 AGATCT A^GATCT BsaAI 226 TACGTG [CT]AC^GT[GA] BsaBI 1132 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 49 GGCGCC G[GA]^CG[CT]C BsaI 753 GAGACC GGTCTC(1/5) BsaWI 623 ACCGGA [AT]^CCGG[AT] BsmFI 66 GTCCC GGGAC(10/14) BsmI 1076 GCATTC GAATGC(1/-1) BspHI 1248 TCATGA T^CATGA BspMI 629 GCAGGT ACCTGC(4/8) BsrBI 123 CCGCTC CCGCTC(-3/-3) BstXI 843 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 302 GGGGCCT [GA]G^G[ACGT]CC[CT] EagI 383 CGGCCG C^GGCCG Eam1105I 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 383 CGGCCG C^GGCCG Eco52I 383 CGGCCG C^GGCCG EcoO109I 302 GGGGCCT [GA]G^G[ACGT]CC[CT] Hin1I 49 GGCGCC G[GA]^CG[CT]C HindIII 901 AAGCTT A^AGCTT KasI 49 GGCGCC G^GCGCC KspI 259 CCGCGG CCGC^GG MamI 1132 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 899 TTAA T^TAA NarI 49 GGCGCC GG^CGCC NheI 520 GCTAGC G^CTAGC PflMI 449 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 950 AACGTT AA^CGTT RcaI 1248 TCATGA T^CATGA SacII 259 CCGCGG CCGC^GG SfaNI 707 GCATC GCATC(5/9) SfcI 1115 CTACAG C^T[GA][CT]AG SfoI 49 GGCGCC GGC^GCC SmaI 68 CCCGGG CCC^GGG SphI 467 GCATGC GCATG^C StuI 203 AGGCCT AGG^CCT Tru9I 899 TTAA T^TAA Van91I 449 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 68 CCCGGG C^CCGGG XmaI 68 CCCGGG C^CCGGG XmnI 81 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 1160 GAATTC [GA]^AATT[CT] 1297 GAATTC ApoI 1160 GAATTC [GA]^AATT[CT] 1297 GAATTC AvaII 476 GGACC G^G[AT]CC 1044 GGACC BbsI 923 GTCTTC GAAGAC(2/6) 982 GTCTTC BfaI 521 CTAG C^TAG 1271 CTAG BpuAI 923 GTCTTC GAAGAC(2/6) 982 GTCTTC BseRI 484 GAGGAG GAGGAG(10/8) 1169 GAGGAG BsgI 112 CTGCAC GTGCAG(16/14) 1237 GTGCAG BsmAI 753 GAGAC GTCTC(1/5) 724 GTCTC BssHII 314 GCGCGC G^CGCGC 418 GCGCGC BstYI 512 AGATCC [GA]^GATC[CT] 1264 AGATCT DdeI 871 CTTAG C^T[ACGT]AG 1022 CTCAG EcoRI 1160 GAATTC G^AATTC 1297 GAATTC EcoT14I 551 CCATGG C^C[AT][AT]GG 998 CCATGG HinfI 854 GAGTC G^A[ACGT]TC 942 GACTC MaeI 521 CTAG C^TAG 1271 CTAG MflI 512 AGATCC [GA]^GATC[CT] 1264 AGATCT MslI 552 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 798 CATCTCTGTG NaeI 107 GCCGGC GCC^GGC 640 GCCGGC NcoI 551 CCATGG C^CATGG 998 CCATGG NgoMIV 107 GCCGGC G^CCGGC 640 GCCGGC NspI 356 GCATGT [GA]CATG^[CT] 467 GCATGC PaeR7I 142 CTCGAG C^TCGAG 322 CTCGAG PleI 942 GACTC GAGTC(4/5) 854 GAGTC SacI 271 GAGCTC GAGCT^C 1252 GAGCTC ScaI 179 AGTACT AGT^ACT 326 AGTACT StyI 551 CCATGG C^C[AT][AT]GG 998 CCATGG Tsp45I 856 GTCAC ^GT[GC]AC 911 GTCAC XcmI 449 CCAGGCGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 491 CCACGTCGTCGCTGG XhoI 142 CTCGAG C^TCGAG 322 CTCGAG XhoII 512 AGATCC [GA]^GATC[CT] 1264 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiEI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmlI TaqI TseI Tsp509I TspRI TthHB8I