(Circular) (Six-base) MAPSORT of: pFN21AE3979.seq Check: 1196 from: 1 to: 6013 pFN21AE3979 6013 bp With 182 enzymes: SgfI * March 28, 2012 11:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 2956 4140 4513 1563 Size: 1393 1184 373 3063 Fragments arranged by size: 3063 1393 1184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2915 4076 5460 5728 1632 Size: 473 810 1161 1384 268 1917 Fragments arranged by size: 1917 1384 1161 810 473 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 2362 4742 2362 Size: 2380 3633 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 2460 2521 3158 3756 5328 2460 Size: 61 637 598 1572 3145 Fragments arranged by size: 3145 1572 637 598 61 ApaLI G'TGCA_C Cuts at: 3018 4070 5226 3018 Size: 1052 1156 3805 Fragments arranged by size: 3805 1156 1052 ApoI r'AATT_y Cuts at: 1493 3165 3302 3322 3548 3700 5809 1493 Size: 1672 137 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 1672 226 152 137 20 AscI GG'CGCG_CC Cuts at: 2423 2423 Size: 6013 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2147 2327 3338 1870 Size: 73 130 74 180 1011 4545 Fragments arranged by size: 4545 1011 180 130 74 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 3334 4789 618 Size: 325 217 627 267 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2280 2496 2664 3261 3332 1391 Size: 889 216 168 597 71 4072 Fragments arranged by size: 4072 889 597 216 168 71 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6013 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2921 2980 928 Size: 971 1022 59 3961 Fragments arranged by size: 3961 1022 971 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3153 3905 5115 3153 Size: 752 1210 4051 Fragments arranged by size: 4051 1210 752 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2316 2444 2590 Size: 107 122 71 631 605 644 128 146 Cuts at: 2590 4624 136 Size: 2034 1525 Fragments arranged by size: 2034 1525 644 631 605 146 128 122 107 71 BglII A'GATC_T Cuts at: 3269 6008 3269 Size: 2739 3274 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 3022 4074 5230 1790 Size: 1232 1052 1156 2573 Fragments arranged by size: 2573 1232 1156 1052 BmgBI CAC'GTC Cuts at: 1318 2143 2499 1318 Size: 825 356 4832 Fragments arranged by size: 4832 825 356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3744 4702 Size: 416 53 83 399 24 897 1430 958 Cuts at: 4702 442 Size: 1753 Fragments arranged by size: 1753 1430 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2529 1056 Size: 1473 4540 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2437 2486 2831 2877 4673 1239 Size: 1094 104 49 345 46 1796 2579 Fragments arranged by size: 2579 1796 1094 345 104 49 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2887 3245 4138 5003 5301 2887 Size: 358 893 865 298 3599 Fragments arranged by size: 3599 893 865 358 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2752 4676 882 Size: 617 1253 1924 2219 Fragments arranged by size: 2219 1924 1253 617 BsaAI yAC'GTr Cuts at: 493 1204 2233 3804 493 Size: 711 1029 1571 2702 Fragments arranged by size: 2702 1571 1029 711 BsaBI GATnn'nnATC Cuts at: 3141 6007 3141 Size: 2866 3147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3820 4202 4837 Size: 53 83 186 936 522 1765 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1765 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2628 4446 4844 5118 5265 5676 2628 Size: 1818 398 274 147 411 2965 Fragments arranged by size: 2965 1818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 3168 3198 1178 Size: 30 165 30 1080 30 655 30 3993 Fragments arranged by size: 3993 1080 655 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 3189 1186 Size: 1318 685 4010 Fragments arranged by size: 4010 1318 685 BseYI C'CCAG_C Cuts at: 2407 5216 5712 2407 Size: 2809 496 2708 Fragments arranged by size: 2809 2708 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 3263 1163 Size: 573 366 1161 3913 Fragments arranged by size: 3913 1161 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 2197 2359 2391 3463 4224 Size: 275 186 169 27 162 32 1072 761 Cuts at: 4224 5252 1540 Size: 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 169 162 32 27 BsiHKAI G_wGCw'C Cuts at: 2148 2280 3022 3261 4074 4159 5230 2148 Size: 132 742 239 813 85 1071 2931 Fragments arranged by size: 2931 1071 813 742 239 132 85 BsmI GAATG_Cn' Cuts at: 3081 3524 3617 3081 Size: 443 93 5477 Fragments arranged by size: 5477 443 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2280 2496 2664 3022 3261 3332 Size: 399 358 132 216 168 358 239 71 Cuts at: 3332 4074 4159 5230 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 358 239 216 168 132 85 71 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3253 3902 5632 3253 Size: 649 1730 3634 Fragments arranged by size: 3634 1730 649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 3368 844 Size: 1781 743 3489 Fragments arranged by size: 3489 1781 743 BsrBI CCG'CTC Cuts at: 1394 1823 2130 3900 1394 Size: 429 307 1770 3507 Fragments arranged by size: 3507 1770 429 307 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 2196 2645 4657 4844 1700 Size: 110 302 84 449 2012 187 2869 Fragments arranged by size: 2869 2012 449 302 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 2319 2423 1925 Size: 394 104 5515 Fragments arranged by size: 5515 394 104 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6013 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstXI CCAn_nnnn'nTGG Cuts at: 2855 2855 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2517 3269 3343 3710 4095 4112 5564 5650 Size: 545 752 74 367 385 17 1452 86 Cuts at: 5650 5662 6008 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 752 545 385 367 346 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2242 2264 2556 3003 513 Size: 552 1177 22 292 447 3523 Fragments arranged by size: 3523 1177 552 447 292 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 2700 3251 3529 4323 4343 2700 Size: 551 278 794 20 4370 Fragments arranged by size: 4370 794 551 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2388 2508 Size: 54 1425 50 275 18 409 149 120 Cuts at: 2508 3460 4349 5978 8 Size: 952 889 1629 43 Fragments arranged by size: 1629 1425 952 889 409 275 149 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2388 3460 1537 Size: 275 576 1072 4090 Fragments arranged by size: 4090 1072 576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3943 1353 Size: 2590 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2494 2632 4600 4974 5120 387 Size: 1064 138 905 138 1968 374 146 1280 Fragments arranged by size: 1968 1280 1064 905 374 146 138 138 EcoICRI GAG'CTC Cuts at: 2278 3259 2278 Size: 981 5032 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2333 2437 2486 2831 2877 2915 Size: 393 473 228 104 49 345 46 38 Cuts at: 2915 4076 4673 5460 5728 1239 Size: 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 787 597 473 393 345 268 228 104 49 46 38 EcoO109I rG'GnC_Cy Cuts at: 2308 2308 Size: 6013 EcoRI G'AATT_C Cuts at: 3165 3302 3322 3700 3165 Size: 137 20 378 5478 Fragments arranged by size: 5478 378 137 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 6013 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 2058 2347 2461 5160 1678 Size: 380 289 114 2699 2531 Fragments arranged by size: 2699 2531 380 289 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2348 2380 2483 Size: 32 135 32 614 32 130 32 103 Cuts at: 2483 2515 4654 4686 4728 4760 1373 Size: 32 2139 32 42 32 2626 Fragments arranged by size: 2626 2139 614 135 130 103 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 2906 748 Size: 2158 3855 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 3087 5127 5311 1305 Size: 1086 696 2040 184 2007 Fragments arranged by size: 2040 2007 1086 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 2807 3971 4330 4489 518 Size: 648 1395 246 1164 359 159 2042 Fragments arranged by size: 2042 1395 1164 648 359 246 159 MspA1I CmG'CkG Cuts at: 1606 2022 2098 2266 3429 4106 5254 5499 1606 Size: 416 76 168 1163 677 1148 245 2120 Fragments arranged by size: 2120 1163 1148 677 416 245 168 76 NaeI GCC'GGC Cuts at: 2114 2647 2114 Size: 533 5480 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6013 NcoI C'CATG_G Cuts at: 513 1065 2556 3003 513 Size: 552 1491 447 3523 Fragments arranged by size: 3523 1491 552 447 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2112 2645 2112 Size: 533 5480 NheI G'CTAG_C Cuts at: 1052 2525 1052 Size: 1473 4540 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2365 2476 3371 4916 1132 Size: 97 1136 111 895 1545 2229 Fragments arranged by size: 2229 1545 1136 895 111 97 PasI CC'CwG_GG Cuts at: 1348 2651 1348 Size: 1303 4710 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2460 1108 Size: 1352 4661 PfoI T'CCnGG_A Cuts at: 2666 2666 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspXI vC'TCGA_Gb Cuts at: 2147 2327 2147 Size: 180 5833 PstI C_TGCA'G Cuts at: 830 3365 830 Size: 2535 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SacI G_AGCT'C Cuts at: 2280 3261 2280 Size: 981 5032 SacII CC_GC'GG Cuts at: 2267 2267 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 2186 2333 4261 5973 2186 Size: 147 1928 1712 2226 Fragments arranged by size: 2226 1928 1712 147 SfcI C'TryA_G Cuts at: 826 1045 3120 3361 4496 5177 5368 826 Size: 219 2075 241 1135 681 191 1471 Fragments arranged by size: 2075 1471 1135 681 241 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6013 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 2075 3340 1872 Size: 203 1265 4545 Fragments arranged by size: 4545 1265 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2327 2866 3260 4117 5018 Size: 197 926 204 180 539 394 857 901 Cuts at: 5018 5280 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 857 539 394 262 204 197 180 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 2476 3371 1132 Size: 1344 895 3774 Fragments arranged by size: 3774 1344 895 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 2210 1517 Size: 693 5320 StyI C'CwwG_G Cuts at: 513 1065 2556 3003 3452 513 Size: 552 1491 447 449 3074 Fragments arranged by size: 3074 1491 552 449 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2887 3091 4040 4057 4210 4395 4840 Size: 1255 1307 204 949 17 153 185 445 Cuts at: 4840 325 Size: 1498 Fragments arranged by size: 1498 1307 1255 949 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2331 4259 Size: 274 80 33 51 1443 207 147 1928 Cuts at: 4259 5971 96 Size: 1712 138 Fragments arranged by size: 1928 1712 1443 274 207 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2503 2461 Size: 42 5971 XhoI C'TCGA_G Cuts at: 1943 2147 2327 1943 Size: 204 180 5629 Fragments arranged by size: 5629 204 180 XmaI C'CCGG_G Cuts at: 1870 2073 3338 1870 Size: 203 1265 4545 Fragments arranged by size: 4545 1265 203 XmnI GAAnn'nnTTC Cuts at: 2090 4142 2090 Size: 2052 3961 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI