################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AvaI AviII AvrII BamHI BanII BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MaeII MamI MluI MroI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1425 GGTACC G^GTACC AhdI 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 34 CCTGAGG CC^T[ACGT]AGG Asp718I 1425 GGTACC G^GTACC AspEI 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 26 GGTCC G^G[AT]CC BalI 958 TGGCCA TGG^CCA BcgI 49 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 29 CCGGG CC^[GC]GG BglII 749 AGATCT A^GATCT BspMI 123 GCAGGT ACCTGC(4/8) BstBI 1372 TTCGAA TT^CGAA BstXI 304 CCAGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 34 CCTGAGG CC^T[ACGT]AGG Eam1105I 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 34 CCTGAGG CC^T[ACGT]AGG HapII 29 CCGG C^CGG HgaI 1360 GCGTC GACGC(5/10) HincII 1362 GTCAAC GT[CT]^[GA]AC HindII 1362 GTCAAC GT[CT]^[GA]AC HpaII 29 CCGG C^CGG KpnI 1425 GGTACC GGTAC^C MfeI 587 CAATTG C^AATTG MluNI 958 TGGCCA TGG^CCA MscI 958 TGGCCA TGG^CCA MspI 29 CCGG C^CGG MunI 587 CAATTG C^AATTG NciI 29 CCGGG CC^[GC]GG NdeI 1473 CATATG CA^TATG NlaIII 1195 CATG CATG^ NspV 1372 TTCGAA TT^CGAA PflFI 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 913 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 994 GACTC GAGTC(4/5) SfuI 1372 TTCGAA TT^CGAA SspI 267 AATATT AAT^ATT TaqI 1373 TCGA T^CGA Tth111I 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1373 TCGA T^CGA Van91I 913 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 562 GAATTT [GA]^AATT[CT] 1390 AAATTC AcyI 43 GGCGCC G[GA]^CG[CT]C 1241 GGCGCC AfaI 1010 GTAC GT^AC 1426 GTAC AlwI 301 GATCC GGATC(4/5) 910 GATCC ApoI 562 GAATTT [GA]^AATT[CT] 1390 AAATTC Asp700I 544 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 559 GAAGAATTTC BbeI 43 GGCGCC GGCGC^C 1241 GGCGCC BfaI 811 CTAG C^TAG 1006 CTAG BpmI 76 CTGGAG CTGGAG(16/14) 514 CTGGAG BsaHI 43 GGCGCC G[GA]^CG[CT]C 1241 GGCGCC BsgI 57 CTGCAC GTGCAG(16/14) 665 CTGCAC BsmFI 476 GGGAC GGGAC(10/14) 1016 GGGAC BstF5I 1269 CATCC GGATG(2/0) 715 GGATG Cfr13I 26 GGTCC G^G[ACGT]CC 31 GGGCC EaeI 116 CGGCCA [CT]^GGCC[GA] 958 TGGCCA EarI 1497 CTCTTC CTCTTC(1/4) 466 GAAGAG FauI 64 GCGGG CCCGC(4/6) 102 GCGGG FokI 1269 CATCC GGATG(9/13) 715 GGATG HaeII 43 GGCGCC [GA]GCGC^[CT] 1241 GGCGCC Hin1I 43 GGCGCC G[GA]^CG[CT]C 1241 GGCGCC KasI 43 GGCGCC G^GCGCC 1241 GGCGCC Ksp632I 1497 CTCTTC CTCTTC(1/4) 466 GAAGAG MaeI 811 CTAG C^TAG 1006 CTAG MslI 760 CATCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1294 CACACAGATG MspA1I 484 CAGCTG C[AC]G^C[GT]G 836 CAGCTG NarI 43 GGCGCC GG^CGCC 1241 GGCGCC PvuII 484 CAGCTG CAG^CTG 836 CAGCTG RsaI 1010 GTAC GT^AC 1426 GTAC Sau96I 26 GGTCC G^G[ACGT]CC 31 GGGCC SfoI 43 GGCGCC GGC^GCC 1241 GGCGCC SmlI 729 CTTGAG C^T[CT][GA]AG 1039 CTTGAG StuI 656 AGGCCT AGG^CCT 1035 AGGCCT Tsp45I 1292 GTCAC ^GT[GC]AC 1407 GTCAC XmnI 544 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 559 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI BanI BbvI BsaJI BsiYI BslI BsmI BsrDI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIV PstI Sau3AI ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII