(Circular) (Six-base) MAPSORT of: pFN21AE1948.seq Check: 3258 from: 1 to: 6214 pFN21AE1948 6214 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3430 3535 3430 Size: 105 6109 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3081 3357 4277 5661 5929 1632 Size: 1449 276 920 1384 268 1917 Fragments arranged by size: 1917 1449 1384 920 276 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 5045 5045 Size: 6214 AhdI GACnn_n'nnGTC Cuts at: 3409 4943 3409 Size: 1534 4680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 3957 5529 3957 Size: 1572 4642 ApaLI G'TGCA_C Cuts at: 4271 5427 4271 Size: 1156 5058 ApoI r'AATT_y Cuts at: 1493 2567 3395 3523 3749 3901 6010 1493 Size: 1074 828 128 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 828 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 3539 1870 Size: 73 1596 4545 Fragments arranged by size: 4545 1596 73 BamHI G'GATC_C Cuts at: 3544 3911 3544 Size: 367 5847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 3246 3430 3535 4990 Size: 325 217 627 261 1198 184 105 1455 Cuts at: 4990 618 Size: 1842 Fragments arranged by size: 1842 1455 1198 627 325 261 217 184 105 BanII G_rGCy'C Cuts at: 1391 3533 1391 Size: 2142 4072 BbeI G_GCGC'C Cuts at: 2052 3250 2052 Size: 1198 5016 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2043 2077 4405 4439 1903 Size: 34 106 34 2328 34 3678 Fragments arranged by size: 3678 2328 106 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4825 136 Size: 107 122 71 631 605 3153 1525 Fragments arranged by size: 3153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2754 6209 2754 Size: 3455 2759 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 4275 5431 1790 Size: 2485 1156 2573 Fragments arranged by size: 2573 2485 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3945 4903 442 Size: 416 53 83 399 24 2528 958 1753 Fragments arranged by size: 2528 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2540 4874 1239 Size: 863 438 2334 2579 Fragments arranged by size: 2579 2334 863 438 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2755 3065 4339 5204 5502 2755 Size: 310 1274 865 298 3467 Fragments arranged by size: 3467 1274 865 310 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4877 882 Size: 617 3378 2219 Fragments arranged by size: 3378 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4005 493 Size: 711 2801 2702 Fragments arranged by size: 2801 2702 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 3247 4021 4403 Size: 53 83 186 936 516 1198 774 382 Cuts at: 4403 5038 275 Size: 635 1451 Fragments arranged by size: 1451 1198 936 774 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4647 5045 5319 5466 5877 4647 Size: 398 274 147 411 4984 Fragments arranged by size: 4984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6214 BseYI C'CCAG_C Cuts at: 5417 5913 5417 Size: 496 5718 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2047 2655 1163 Size: 573 311 608 4722 Fragments arranged by size: 4722 608 573 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4275 4360 5431 4275 Size: 85 1071 5058 Fragments arranged by size: 5058 1071 85 BsmI GAATG_Cn' Cuts at: 2329 3171 3353 3446 3725 3818 2329 Size: 842 182 93 279 93 4725 Fragments arranged by size: 4725 842 279 182 93 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3533 4275 4360 5431 1391 Size: 399 1743 742 85 1071 2174 Fragments arranged by size: 2174 1743 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 3569 844 Size: 1275 1450 3489 Fragments arranged by size: 3489 1450 1275 BsrBI CCG'CTC Cuts at: 1394 1823 4101 1394 Size: 429 2278 3507 Fragments arranged by size: 3507 2278 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2430 2877 3089 4709 4883 65 Size: 1183 1182 447 212 1620 174 1396 Fragments arranged by size: 1620 1396 1183 1182 447 212 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4858 5045 1700 Size: 110 3048 187 2869 Fragments arranged by size: 3048 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6214 BstBI TT'CG_AA Cuts at: 3378 5018 3378 Size: 1640 4574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6214 BstXI CCAn_nnnn'nTGG Cuts at: 2316 2316 Size: 6214 BstYI r'GATC_y Cuts at: 1972 2305 2754 2914 3544 3911 4296 4313 5765 Size: 333 449 160 630 367 385 17 1452 Cuts at: 5765 5851 5863 6209 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 630 449 385 367 346 333 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2040 Size: 6214 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5475 Fragments arranged by size: 5475 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2650 2667 3730 4524 4544 2064 Size: 586 17 1063 794 20 3734 Fragments arranged by size: 3734 1063 794 586 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2113 2148 529 Size: 35 1549 35 4595 Fragments arranged by size: 4595 1549 35 35 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2963 3661 Size: 54 1425 50 275 18 291 842 698 Cuts at: 3661 4550 6179 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 842 698 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 3508 4144 1353 Size: 1113 1042 636 3423 Fragments arranged by size: 3423 1113 1042 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3222 4801 5175 5321 387 Size: 1064 138 1633 1579 374 146 1280 Fragments arranged by size: 1633 1579 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2540 3081 3357 4277 4874 5661 Size: 393 470 438 541 276 920 597 787 Cuts at: 5661 5929 1239 Size: 268 1524 Fragments arranged by size: 1524 920 787 597 541 470 438 393 276 268 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3473 3473 Size: 6214 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 2052 3250 5361 1678 Size: 374 1198 2111 2531 Fragments arranged by size: 2531 2111 1198 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2765 2797 2817 Size: 32 135 32 443 32 718 32 20 Cuts at: 2817 2849 3395 3427 4855 4887 4929 4961 1373 Size: 32 546 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 718 546 443 135 42 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3558 3804 669 Size: 988 281 1431 189 246 3079 Fragments arranged by size: 3079 1431 988 281 246 189 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KasI G'GCGC_C Cuts at: 2048 3246 2048 Size: 1198 5016 KpnI G_GTAC'C Cuts at: 3434 3539 3434 Size: 105 6109 MfeI C'AATT_G Cuts at: 2592 3813 2592 Size: 1221 4993 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5328 5512 1305 Size: 684 3339 184 2007 Fragments arranged by size: 3339 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2965 10 Size: 54 1425 343 1133 3259 Fragments arranged by size: 3259 1425 1133 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2769 3303 4172 4531 4690 518 Size: 648 1603 534 869 359 159 2042 Fragments arranged by size: 2042 1603 869 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 2843 3630 4307 5455 5700 1606 Size: 885 352 787 677 1148 245 2120 Fragments arranged by size: 2120 1148 885 787 677 352 245 NarI GG'CG_CC Cuts at: 2049 3247 2049 Size: 1198 5016 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5662 NdeI CA'TA_TG Cuts at: 387 3479 387 Size: 3092 3122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6214 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 2924 1108 Size: 1816 4398 PfoI T'CCnGG_A Cuts at: 2308 2308 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6214 PsiI TTA'TAA Cuts at: 2297 3784 2297 Size: 1487 4727 PstI C_TGCA'G Cuts at: 830 2206 2645 3040 3566 830 Size: 1376 439 395 526 3478 Fragments arranged by size: 3478 1376 526 439 395 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2491 2843 2491 Size: 352 5862 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SbfI CC_TGCA'GG Cuts at: 3566 3566 Size: 6214 ScaI AGT'ACT Cuts at: 4462 6174 4462 Size: 1712 4502 SfcI C'TryA_G Cuts at: 826 1045 2202 2641 3036 3562 4697 5378 5569 Size: 219 1157 439 395 526 1135 681 191 Cuts at: 5569 826 Size: 1471 Fragments arranged by size: 1471 1157 1135 681 526 439 395 219 191 SfoI GGC'GCC Cuts at: 2050 3248 2050 Size: 1198 5016 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2734 3044 4318 5219 5481 820 Size: 197 926 791 310 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 791 310 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 2274 4138 5 Size: 47 2222 1864 2081 Fragments arranged by size: 2222 2081 1864 47 StuI AGG'CCT Cuts at: 1517 2663 3042 1517 Size: 1146 379 4689 Fragments arranged by size: 4689 1146 379 StyI C'CwwG_G Cuts at: 513 1065 3288 3426 3457 3653 513 Size: 552 2223 138 31 196 3074 Fragments arranged by size: 3074 2223 552 196 138 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4241 4258 4411 4596 5041 325 Size: 1255 2661 17 153 185 445 1498 Fragments arranged by size: 2661 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4460 6172 96 Size: 274 80 33 51 1443 2483 1712 138 Fragments arranged by size: 2483 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 6182 Tth111I GACn'n_nGTC Cuts at: 1316 3364 1316 Size: 2048 4166 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6214 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 2553 2568 4343 2553 Size: 15 1775 4424 Fragments arranged by size: 4424 1775 15 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI