################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BbrPI BcgI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BspEI BspLU11I BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HgaI HpaI KpnI KspI MroI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SfuI SgrAI SmaI SnaBI SphI SspI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccII 1834 CGCG CG^CG AcyI 212 GGCGCC G[GA]^CG[CT]C AflII 1495 CTTAAG C^TTAAG AflIII 1833 ACGCGT A^C[GA][CT]GT ApaI 3563 GGGCCC GGGCC^C AseI 2487 ATTAAT AT^TAAT AsnI 2487 ATTAAT AT^TAAT AspHI 3038 GTGCTC G[AT]GC[AT]^C AvaI 4109 CTCGAG C^[CT]CG[GA]G BanI 212 GGCGCC G^G[CT][GA]CC BbeI 212 GGCGCC GGCGC^C BbvCI 1907 CCTCAGC CCTCAGC(-5/-2) BcnI 4118 CCCGG CC^[GC]GG BfrI 1495 CTTAAG C^TTAAG BglII 692 AGATCT A^GATCT BsaHI 212 GGCGCC G[GA]^CG[CT]C BsaI 347 GAGACC GGTCTC(1/5) BsiHKAI 3038 GTGCTC G[AT]GC[AT]^C BsoBI 4109 CTCGAG C^[CT]CG[GA]G BspHI 2010 TCATGA T^CATGA BstUI 1834 CGCG CG^CG CfoI 213 GCGC GCG^C DraI 2958 TTTAAA TTT^AAA DraII 3562 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 1678 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 3562 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 3636 GATATC GAT^ATC EcoT14I 3567 CCAAGG C^C[AT][AT]GG EcoT22I 1043 ATGCAT ATGCA^T HaeII 212 GGCGCC [GA]GCGC^[CT] HapII 4119 CCGG C^CGG HhaI 213 GCGC GCG^C Hin1I 212 GGCGCC G[GA]^CG[CT]C HinP1I 213 GCGC G^CGC HincII 706 GTCAAC GT[CT]^[GA]AC HindII 706 GTCAAC GT[CT]^[GA]AC HpaII 4119 CCGG C^CGG KasI 212 GGCGCC G^GCGCC MaeII 2628 ACGT A^CGT MluI 1833 ACGCGT A^CGCGT MspI 4119 CCGG C^CGG MvnI 1834 CGCG CG^CG NarI 212 GGCGCC GG^CGCC NciI 4118 CCCGG CC^[GC]GG NdeI 304 CATATG CA^TATG NsiI 1043 ATGCAT ATGCA^T NspI 1542 ACATGC [GA]CATG^[CT] PaeR7I 4109 CTCGAG C^TCGAG PflMI 4011 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2487 ATTAAT AT^TAAT RcaI 2010 TCATGA T^CATGA SbfI 2707 CCTGCAGG CCTGCA^GG ScaI 3715 AGTACT AGT^ACT SfiI 2887 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 212 GGCGCC GGC^GCC SpeI 3865 ACTAGT A^CTAGT Sse8387I 2707 CCTGCAGG CCTGCA^GG StyI 3567 CCAAGG C^C[AT][AT]GG Van91I 4011 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 4109 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AocI 606 CCTGAGG CC^T[ACGT]AGG 2328 CCTAAGG Asp700I 793 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1651 GAAACCATTC BalI 445 TGGCCA TGG^CCA 3476 TGGCCA BciVI 1876 GGATAC GTATCC(6/5) 3663 GTATCC BclI 2181 TGATCA T^GATCA 4053 TGATCA BglI 2888 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3265 GCCAGTTTGGC BlpI 503 GCTTAGC GC^T[ACGT]AGC 2113 GCTGAGC Bpu1102I 503 GCTTAGC GC^T[ACGT]AGC 2113 GCTGAGC BsaBI 687 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1018 GATTCTCATC BsmI 3731 GAATGC GAATGC(1/-1) 3343 GCATTC Bsp1407I 888 TGTACA T^GTACA 3783 TGTACA BspMI 350 ACCTGC ACCTGC(4/8) 3678 GCAGGT BsrBI 1108 CCGCTC CCGCTC(-3/-3) 436 GAGCGG BsrGI 888 TGTACA T^GTACA 3783 TGTACA BssSI 3850 CACGAG CACGAG(-5/-1) 1111 CTCGTG Bsu36I 606 CCTGAGG CC^T[ACGT]AGG 2328 CCTAAGG CelII 503 GCTTAGC GC^T[ACGT]AGC 2113 GCTGAGC EaeI 445 TGGCCA [CT]^GGCC[GA] 3476 TGGCCA Eco81I 606 CCTGAGG CC^T[ACGT]AGG 2328 CCTAAGG EcoNI 1239 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2704 CCTCCTGCAGG EcoRI 797 GAATTC G^AATTC 897 GAATTC FauI 2414 CCCGC CCCGC(4/6) 3909 CCCGC FbaI 2181 TGATCA T^GATCA 4053 TGATCA HindIII 717 AAGCTT A^AGCTT 2278 AAGCTT MamI 687 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1018 GATTCTCATC MfeI 2204 CAATTG C^AATTG 4133 CAATTG MluNI 445 TGGCCA TGG^CCA 3476 TGGCCA MscI 445 TGGCCA TGG^CCA 3476 TGGCCA MunI 2204 CAATTG C^AATTG 4133 CAATTG SapI 2677 GAAGAGC GCTCTTC(1/4) 337 GCTCTTC SexAI 1679 ACCAGGT A^CC[AT]GGT 2160 ACCAGGT SspBI 888 TGTACA T^GTACA 3783 TGTACA XbaI 58 TCTAGA T^CTAGA 2841 TCTAGA XcmI 2822 CCAGCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3567 CCAAGGAGGAGATGG XmnI 793 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1651 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BamHI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BspDI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I ClaI DdeI DpnII DsaI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII