(Circular) (Six-base) MAPSORT of: pFN21AE2330.seq Check: 699 from: 1 to: 8863 pFN21AE2330 8863 bp With 182 enzymes: SgfI * April 25, 2011 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5674 5674 Size: 8863 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6673 278 Size: 53 83 186 936 5137 2468 Fragments arranged by size: 5137 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6206 1937 Size: 4269 4594 Acc65I G'GTAC_C Cuts at: 6184 6184 Size: 8863 AclI AA'CG_TT Cuts at: 1563 6990 7363 1563 Size: 5427 373 3063 Fragments arranged by size: 5427 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1998 2188 2282 6926 8310 8578 1632 Size: 366 190 94 4644 1384 268 1917 Fragments arranged by size: 4644 1917 1384 366 268 190 94 AflII C'TTAA_G Cuts at: 820 1017 3500 820 Size: 197 2483 6183 Fragments arranged by size: 6183 2483 197 AflIII A'CryG_T Cuts at: 1203 3838 7762 8739 1203 Size: 2635 3924 977 1327 Fragments arranged by size: 3924 2635 1327 977 AgeI A'CCGG_T Cuts at: 7694 7694 Size: 8863 AhdI GACnn_n'nnGTC Cuts at: 7592 7592 Size: 8863 AleI CACnn'nnGTG Cuts at: 1166 3944 6140 1166 Size: 2778 2196 3889 Fragments arranged by size: 3889 2778 2196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8863 AlwNI CAG_nnn'CTG Cuts at: 2817 3434 4414 6606 8178 2817 Size: 617 980 2192 1572 3502 Fragments arranged by size: 3502 2192 1572 980 617 ApaI G_GGCC'C Cuts at: 5572 5572 Size: 8863 ApaLI G'TGCA_C Cuts at: 6920 8076 6920 Size: 1156 7707 ApoI r'AATT_y Cuts at: 1493 2537 2789 2802 2902 2921 2945 3072 3405 Size: 1044 252 13 100 19 24 127 333 Cuts at: 3405 3732 5025 5342 5705 6172 6398 6550 8659 Size: 327 1293 317 363 467 226 152 2109 Cuts at: 8659 1493 Size: 1697 Fragments arranged by size: 2109 1697 1293 1044 467 363 333 327 317 252 226 152 127 100 24 19 13 AseI AT'TA_AT Cuts at: 160 1032 4493 7417 160 Size: 872 3461 2924 1606 Fragments arranged by size: 3461 2924 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8863 AvaI C'yCGr_G Cuts at: 1870 1943 6114 6188 1870 Size: 73 4171 74 4545 Fragments arranged by size: 4545 4171 74 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2182 2215 2825 2858 4845 4878 2182 Size: 33 610 33 1987 33 6167 Fragments arranged by size: 6167 1987 610 33 33 33 BamHI G'GATC_C Cuts at: 2488 5248 5994 6119 6193 6560 2488 Size: 2760 746 125 74 367 4791 Fragments arranged by size: 4791 2760 746 367 125 74 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2217 6184 7639 618 Size: 325 217 627 430 3967 1455 1842 Fragments arranged by size: 3967 1842 1455 627 430 325 217 BanII G_rGCy'C Cuts at: 1391 4247 5544 5572 6182 1391 Size: 2856 1297 28 610 4072 Fragments arranged by size: 4072 2856 1297 610 28 BbeI G_GCGC'C Cuts at: 2221 2221 Size: 8863 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2052 4076 4335 5720 928 Size: 971 153 2024 259 1385 4071 Fragments arranged by size: 4071 2024 1385 971 259 153 BbvCI CC'TCA_GC Cuts at: 3913 3913 Size: 8863 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7054 7088 1903 Size: 34 5117 34 3678 Fragments arranged by size: 5117 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5679 6755 7965 3875 Size: 1804 1076 1210 4773 Fragments arranged by size: 4773 1804 1210 1076 BclI T'GATC_A Cuts at: 1548 4186 6058 1548 Size: 2638 1872 4353 Fragments arranged by size: 4353 2638 1872 BfrBI ATG'CAT Cuts at: 3050 3050 Size: 8863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4899 5276 7474 Size: 107 122 71 631 605 3227 377 2198 Cuts at: 7474 136 Size: 1525 Fragments arranged by size: 3227 2198 1525 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 2697 8858 2697 Size: 6161 2702 BlpI GC'TnA_GC Cuts at: 2509 4119 6280 2509 Size: 1610 2161 5092 Fragments arranged by size: 5092 2161 1610 Bme1580I G_kGCm'C Cuts at: 1790 2982 5572 6924 8080 1790 Size: 1192 2590 1352 1156 2573 Fragments arranged by size: 2590 2573 1352 1192 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2656 6594 7552 Size: 416 53 83 399 24 1239 3938 958 Cuts at: 7552 442 Size: 1753 Fragments arranged by size: 3938 1753 1239 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4125 5632 4125 Size: 1507 7356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2858 4337 4527 5039 5130 5745 6053 Size: 910 709 1479 190 512 91 615 308 Cuts at: 6053 7523 1239 Size: 1470 2579 Fragments arranged by size: 2579 1479 1470 910 709 615 512 308 190 91 Bpu10I CC'TnA_GC Cuts at: 3913 3913 Size: 8863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 4851 6988 7853 8151 2753 Size: 2098 2137 865 298 3465 Fragments arranged by size: 3465 2137 2098 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2346 7526 882 Size: 617 847 5180 2219 Fragments arranged by size: 5180 2219 847 617 BsaAI yAC'GTr Cuts at: 493 1204 6654 493 Size: 711 5450 2702 Fragments arranged by size: 5450 2702 711 BsaBI GATnn'nnATC Cuts at: 2696 3027 8857 2696 Size: 331 5830 2702 Fragments arranged by size: 5830 2702 331 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2218 6670 7052 7687 Size: 53 83 186 936 685 4452 382 635 Cuts at: 7687 275 Size: 1451 Fragments arranged by size: 4452 1451 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7296 7694 7968 8115 8526 7296 Size: 398 274 147 411 7633 Fragments arranged by size: 7633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5571 5601 5925 5955 5985 Size: 30 165 30 4168 30 324 30 30 Cuts at: 5985 1178 Size: 4056 Fragments arranged by size: 4168 4056 324 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2082 2091 2420 3452 5592 5946 5976 1186 Size: 896 9 329 1032 2140 354 30 4073 Fragments arranged by size: 4073 2140 1032 896 354 329 30 9 BseYI C'CCAG_C Cuts at: 8066 8562 8066 Size: 496 8367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3447 3919 5702 5969 1163 Size: 573 1711 472 1783 267 4057 Fragments arranged by size: 4057 1783 1711 573 472 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6313 7074 8102 1540 Size: 275 186 4312 761 1028 2301 Fragments arranged by size: 4312 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5047 6924 7009 8080 5047 Size: 1877 85 1071 5830 Fragments arranged by size: 5830 1877 1071 85 BsmI GAATG_Cn' Cuts at: 5348 5742 6374 6467 5348 Size: 394 632 93 7744 Fragments arranged by size: 7744 632 394 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2982 4247 5047 5544 5572 6182 6924 Size: 399 1192 1265 800 497 28 610 742 Cuts at: 6924 7009 8080 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1265 1192 1071 800 742 610 497 399 85 28 BspEI T'CCGG_A Cuts at: 8526 8526 Size: 8863 BspHI T'CATG_A Cuts at: 4015 6752 8482 4015 Size: 2737 1730 4396 Fragments arranged by size: 4396 2737 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 5674 6218 844 Size: 1520 3310 544 3489 Fragments arranged by size: 3489 3310 1520 544 BsrBI CCG'CTC Cuts at: 1394 1823 2443 3115 6750 1394 Size: 429 620 672 3635 3507 Fragments arranged by size: 3635 3507 672 620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4746 5262 5655 7358 7532 65 Size: 1183 3498 516 393 1703 174 1396 Fragments arranged by size: 3498 1703 1396 1183 516 393 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7507 7694 1700 Size: 110 5697 187 2869 Fragments arranged by size: 5697 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2893 5788 96 Size: 2797 2895 3171 Fragments arranged by size: 3171 2895 2797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8863 BssSI C'ACGA_G Cuts at: 3116 5855 6923 7935 3116 Size: 2739 1068 1012 4044 Fragments arranged by size: 4044 2739 1068 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8863 BstBI TT'CG_AA Cuts at: 7667 7667 Size: 8863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8863 BstYI r'GATC_y Cuts at: 1972 2381 2488 2697 5248 5994 6119 6193 6560 Size: 409 107 209 2551 746 125 74 367 Cuts at: 6560 6945 6962 8414 8500 8512 8858 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2551 1977 1452 746 409 385 367 346 209 125 107 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2612 4334 2612 Size: 1722 7141 BtgI C'CryG_G Cuts at: 513 1065 3876 3939 5193 513 Size: 552 2811 63 1254 4183 Fragments arranged by size: 4183 2811 1254 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8124 Fragments arranged by size: 8124 683 56 BtsI GCAGTG_nn' Cuts at: 2565 4386 6379 7173 7193 2565 Size: 1821 1993 794 20 4235 Fragments arranged by size: 4235 1993 1821 794 20 ClaI AT'CG_AT Cuts at: 1554 4642 4770 4791 1554 Size: 3088 128 21 5626 Fragments arranged by size: 5626 3088 128 21 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2029 2064 4729 4764 529 Size: 35 1465 35 2665 35 4628 Fragments arranged by size: 4628 2665 1465 35 35 35 DraI TTT'AAA Cuts at: 4965 6167 4965 Size: 1202 7661 DraIII CAC_nnn'GTG Cuts at: 3688 3688 Size: 8863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7870 809 Size: 507 6554 1802 Fragments arranged by size: 6554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2450 5481 6310 Size: 54 1425 50 275 18 620 3031 829 Cuts at: 6310 7199 8828 8 Size: 889 1629 43 Fragments arranged by size: 3031 1629 1425 889 829 620 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6310 1537 Size: 275 4498 4090 Fragments arranged by size: 4498 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 2349 2748 3249 4677 5106 5458 5601 Size: 715 281 399 501 1428 429 352 143 Cuts at: 5601 6793 1353 Size: 1192 3423 Fragments arranged by size: 3423 1428 1192 715 501 429 399 352 281 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 7450 7824 7970 387 Size: 1064 138 2723 3138 374 146 1280 Fragments arranged by size: 3138 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2149 2188 2282 2858 4337 4527 Size: 393 366 151 39 94 576 1479 190 Cuts at: 4527 5039 5130 5745 6053 6926 7523 8310 8578 Size: 512 91 615 308 873 597 787 268 Cuts at: 8578 1239 Size: 1524 Fragments arranged by size: 1524 1479 873 787 615 597 576 512 393 366 308 268 190 151 94 91 39 EcoNI CCTnn'n_nnAGG Cuts at: 3248 4713 3248 Size: 1465 7398 EcoO109I rG'GnC_Cy Cuts at: 5568 5568 Size: 8863 EcoRI G'AATT_C Cuts at: 2802 2902 6172 6550 2802 Size: 100 3270 378 5115 Fragments arranged by size: 5115 3270 378 100 EcoRV GAT'ATC Cuts at: 5643 5643 Size: 8863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2238 2312 2238 Size: 74 8789 FspI TGC'GCA Cuts at: 7369 7369 Size: 8863 HaeII r_GCGC'y Cuts at: 1678 2221 8010 1678 Size: 543 5789 2531 Fragments arranged by size: 5789 2531 543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 3242 3274 3614 Size: 32 135 32 794 32 844 32 340 Cuts at: 3614 3646 3853 3885 4052 4066 4084 4098 4828 Size: 32 207 32 167 14 18 14 730 Cuts at: 4828 4860 5571 5603 5868 5900 6111 6143 7504 Size: 32 711 32 265 32 211 32 1361 Cuts at: 7504 7536 7578 7610 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1361 844 794 730 711 340 265 211 207 167 135 42 32 32 32 32 32 32 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 2713 6207 6453 669 Size: 988 281 775 3494 246 3079 Fragments arranged by size: 3494 3079 988 775 281 246 HindIII A'AGCT_T Cuts at: 748 2722 4283 748 Size: 1974 1561 5328 Fragments arranged by size: 5328 1974 1561 HpaI GTT'AAC Cuts at: 6453 6453 Size: 8863 KasI G'GCGC_C Cuts at: 2217 2217 Size: 8863 KpnI G_GTAC'C Cuts at: 6188 6188 Size: 8863 MfeI C'AATT_G Cuts at: 4209 6138 6462 4209 Size: 1929 324 6610 Fragments arranged by size: 6610 1929 324 MluI A'CGCG_T Cuts at: 3838 8739 3838 Size: 4901 3962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2977 3240 5972 7977 8161 1305 Size: 1672 263 2732 2005 184 2007 Fragments arranged by size: 2732 2007 2005 1672 263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2452 5483 10 Size: 54 1425 343 620 3031 3390 Fragments arranged by size: 3390 3031 1425 620 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2109 3033 3944 6140 6821 7180 7339 Size: 648 943 924 911 2196 681 359 159 Cuts at: 7339 518 Size: 2042 Fragments arranged by size: 2196 2042 943 924 911 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2456 5008 5241 5753 5917 6279 6956 8104 Size: 850 2552 233 512 164 362 677 1148 Cuts at: 8104 8349 1606 Size: 245 2120 Fragments arranged by size: 2552 2120 1148 850 677 512 362 245 233 164 NarI GG'CG_CC Cuts at: 2218 2218 Size: 8863 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8311 NdeI CA'TA_TG Cuts at: 387 2310 387 Size: 1923 6940 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8863 NotI GC'GGCC_GC Cuts at: 6310 6310 Size: 8863 NsiI A_TGCA'T Cuts at: 3052 3052 Size: 8863 NspI r_CATG'y Cuts at: 1132 1229 3551 6221 7766 1132 Size: 97 2322 2670 1545 2229 Fragments arranged by size: 2670 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 5304 1348 Size: 3956 4907 PciI A'CATG_T Cuts at: 7762 7762 Size: 8863 PflMI CCAn_nnn'nTGG Cuts at: 1108 6022 1108 Size: 4914 3949 PfoI T'CCnGG_A Cuts at: 4353 6069 6122 4353 Size: 1716 53 7094 Fragments arranged by size: 7094 1716 53 PmeI GTTT'AAAC Cuts at: 6167 6167 Size: 8863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2457 2489 6932 6964 8445 8477 2457 Size: 32 4443 32 1481 32 2843 Fragments arranged by size: 4443 2843 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8863 PsiI TTA'TAA Cuts at: 4733 5433 6433 4733 Size: 700 1000 7163 Fragments arranged by size: 7163 1000 700 PspOMI G'GGCC_C Cuts at: 5568 5568 Size: 8863 PspXI vC'TCGA_Gb Cuts at: 6114 6114 Size: 8863 PstI C_TGCA'G Cuts at: 830 4717 5444 5475 5897 6215 830 Size: 3887 727 31 422 318 3478 Fragments arranged by size: 3887 3478 727 422 318 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8863 PvuII CAG'CTG Cuts at: 2456 5008 5753 2456 Size: 2552 745 5566 Fragments arranged by size: 5566 2552 745 SalI G'TCGA_C Cuts at: 1936 6205 1936 Size: 4269 4594 SapI GCTCTTCn'nnn_ Cuts at: 2349 4677 2349 Size: 2328 6535 SbfI CC_TGCA'GG Cuts at: 4717 6215 4717 Size: 1498 7365 ScaI AGT'ACT Cuts at: 5722 7111 8823 5722 Size: 1389 1712 5762 Fragments arranged by size: 5762 1712 1389 SexAI A'CCwGG_T Cuts at: 3684 4165 3684 Size: 481 8382 SfcI C'TryA_G Cuts at: 826 1045 4604 4713 5440 5471 5893 6211 7346 Size: 219 3559 109 727 31 422 318 1135 Cuts at: 7346 8027 8218 826 Size: 681 191 1471 Fragments arranged by size: 3559 1471 1135 727 681 422 318 219 191 109 31 SfiI GGCCn_nnn'nGGCC Cuts at: 4899 4899 Size: 8863 SfoI GGC'GCC Cuts at: 2219 2219 Size: 8863 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8863 SmaI CCC'GGG Cuts at: 1872 6190 1872 Size: 4318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 3500 4866 6114 6967 7868 Size: 197 926 825 732 1366 1248 853 901 Cuts at: 7868 8130 820 Size: 262 1553 Fragments arranged by size: 1553 1366 1248 926 901 853 825 732 262 197 SnaBI TAC'GTA Cuts at: 493 6654 493 Size: 6161 2702 SpeI A'CTAG_T Cuts at: 152 5870 152 Size: 5718 3145 SphI G_CATG'C Cuts at: 1132 6221 1132 Size: 5089 3774 SspI AAT'ATT Cuts at: 5 52 6787 5 Size: 47 6735 2081 Fragments arranged by size: 6735 2081 47 StuI AGG'CCT Cuts at: 1517 2162 2394 3194 4502 1517 Size: 645 232 800 1308 5878 Fragments arranged by size: 5878 1308 800 645 232 StyI C'CwwG_G Cuts at: 513 1065 5572 6302 513 Size: 552 4507 730 3074 Fragments arranged by size: 4507 3074 730 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4671 6890 6907 7060 7245 7690 325 Size: 1255 3091 2219 17 153 185 445 1498 Fragments arranged by size: 3091 2219 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2893 3544 5405 Size: 274 80 33 51 1443 916 651 1861 Cuts at: 5405 5720 5788 7109 8821 96 Size: 315 68 1321 1712 138 Fragments arranged by size: 1861 1712 1443 1321 916 651 315 274 138 80 68 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3718 3750 4994 5026 5925 5957 8823 8855 3718 Size: 32 1244 32 899 32 2866 32 3726 Fragments arranged by size: 3726 2866 1244 899 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8863 XbaI T'CTAG_A Cuts at: 2063 4846 6199 2063 Size: 2783 1353 4727 Fragments arranged by size: 4727 2783 1353 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4834 5579 4834 Size: 745 8118 XhoI C'TCGA_G Cuts at: 1943 6114 1943 Size: 4171 4692 XmaI C'CCGG_G Cuts at: 1870 6188 1870 Size: 4318 4545 XmnI GAAnn'nnTTC Cuts at: 2802 3660 6992 2802 Size: 858 3332 4673 Fragments arranged by size: 4673 3332 858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6671 276 Size: 53 83 186 936 5137 2468 Fragments arranged by size: 5137 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SrfI SwaI