Restriction map for
pFN21AE2330
(Linear) (Six-base) MAPSORT of: KIEE2330.seq Check: 2150 from: 1 to: 4158
KIEE2330.orf
With 182 enzymes: SgfI *
April 25, 2011 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3669 4158
Size: 3669 489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 277 4158
Size: 183 94 3881
Fragments arranged by size:
3881 183 94
AflII C'TTAA_G
Cuts at: 0 1495 4158
Size: 1495 2663
AflIII A'CryG_T
Cuts at: 0 1833 4158
Size: 1833 2325
AleI CACnn'nnGTG
Cuts at: 0 1939 4135 4158
Size: 1939 2196 23
Fragments arranged by size:
2196 1939 23
AlwNI CAG_nnn'CTG
Cuts at: 0 812 1429 2409 4158
Size: 812 617 980 1749
Fragments arranged by size:
1749 980 812 617
ApaI G_GGCC'C
Cuts at: 0 3567 4158
Size: 3567 591
ApoI r'AATT_y
Cuts at: 0 532 784 797 897 916 940 1067 1400
Size: 532 252 13 100 19 24 127 333
Cuts at: 1400 1727 3020 3337 3700 4158
Size: 327 1293 317 363 458
Fragments arranged by size:
1293 532 458 363 333 327 317 252
127 100 24 19 13
AseI AT'TA_AT
Cuts at: 0 2488 4158
Size: 2488 1670
AvaI C'yCGr_G
Cuts at: 0 4109 4158
Size: 4109 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 177 210 820 853 2840 2873 4158
Size: 177 33 610 33 1987 33 1285
Fragments arranged by size:
1987 1285 610 177 33 33 33
BamHI G'GATC_C
Cuts at: 0 483 3243 3989 4114 4158
Size: 483 2760 746 125 44
Fragments arranged by size:
2760 746 483 125 44
BanI G'GyrC_C
Cuts at: 0 212 4158
Size: 212 3946
BanII G_rGCy'C
Cuts at: 0 2242 3539 3567 4158
Size: 2242 1297 28 591
Fragments arranged by size:
2242 1297 591 28
BbeI G_GCGC'C
Cuts at: 0 216 4158
Size: 216 3942
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 2071 2330 3715 4158
Size: 47 2024 259 1385 443
Fragments arranged by size:
2024 1385 443 259 47
BbvCI CC'TCA_GC
Cuts at: 0 1908 4158
Size: 1908 2250
BciVI GTATCCnnnnn_n'
Cuts at: 0 1870 3674 4158
Size: 1870 1804 484
Fragments arranged by size:
1870 1804 484
BclI T'GATC_A
Cuts at: 0 2181 4053 4158
Size: 2181 1872 105
Fragments arranged by size:
2181 1872 105
BfrBI ATG'CAT
Cuts at: 0 1045 4158
Size: 1045 3113
BglI GCCn_nnn'nGGC
Cuts at: 0 2894 3271 4158
Size: 2894 377 887
Fragments arranged by size:
2894 887 377
BglII A'GATC_T
Cuts at: 0 692 4158
Size: 692 3466
BlpI GC'TnA_GC
Cuts at: 0 504 2114 4158
Size: 504 1610 2044
Fragments arranged by size:
2044 1610 504
Bme1580I G_kGCm'C
Cuts at: 0 977 3567 4158
Size: 977 2590 591
Fragments arranged by size:
2590 977 591
BmrI ACTGGGnnnn_n'
Cuts at: 0 651 4158
Size: 651 3507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2120 3627 4158
Size: 2120 1507 531
Fragments arranged by size:
2120 1507 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 853 2332 2522 3034 3125 3740 4048
Size: 144 709 1479 190 512 91 615 308
Cuts at: 4048 4158
Size: 110
Fragments arranged by size:
1479 709 615 512 308 190 144 110
91
Bpu10I CC'TnA_GC
Cuts at: 0 1908 4158
Size: 1908 2250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 2846 4158
Size: 748 2098 1312
Fragments arranged by size:
2098 1312 748
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 4158
Size: 341 3817
BsaBI GATnn'nnATC
Cuts at: 0 691 1022 4158
Size: 691 331 3136
Fragments arranged by size:
3136 691 331
BsaHI Gr'CG_yC
Cuts at: 0 213 4158
Size: 213 3945
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3566 3596 3920 3950 3980 4158
Size: 3566 30 324 30 30 178
Fragments arranged by size:
3566 324 178 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 86 415 1447 3587 3941 3971 4158
Size: 77 9 329 1032 2140 354 30 187
Fragments arranged by size:
2140 1032 354 329 187 77 30 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1442 1914 3697 3964 4158
Size: 1442 472 1783 267 194
Fragments arranged by size:
1783 1442 472 267 194
BsiHKAI G_wGCw'C
Cuts at: 0 3042 4158
Size: 3042 1116
BsmI GAATG_Cn'
Cuts at: 0 3343 3737 4158
Size: 3343 394 421
Fragments arranged by size:
3343 421 394
Bsp1286I G_dGCh'C
Cuts at: 0 977 2242 3042 3539 3567 4158
Size: 977 1265 800 497 28 591
Fragments arranged by size:
1265 977 800 591 497 28
BspHI T'CATG_A
Cuts at: 0 2010 4158
Size: 2010 2148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 3669 4158
Size: 359 3310 489
Fragments arranged by size:
3310 489 359
BsrBI CCG'CTC
Cuts at: 0 438 1110 4158
Size: 438 672 3048
Fragments arranged by size:
3048 672 438
BsrDI GCAATG_nn'
Cuts at: 0 2741 3257 3650 4158
Size: 2741 516 393 508
Fragments arranged by size:
2741 516 508 393
BsrGI T'GTAC_A
Cuts at: 0 888 3783 4158
Size: 888 2895 375
Fragments arranged by size:
2895 888 375
BssSI C'ACGA_G
Cuts at: 0 1111 3850 4158
Size: 1111 2739 308
Fragments arranged by size:
2739 1111 308
BstYI r'GATC_y
Cuts at: 0 376 483 692 3243 3989 4114 4158
Size: 376 107 209 2551 746 125 44
Fragments arranged by size:
2551 746 376 209 125 107 44
Bsu36I CC'TnA_GG
Cuts at: 0 607 2329 4158
Size: 607 1722 1829
Fragments arranged by size:
1829 1722 607
BtgI C'CryG_G
Cuts at: 0 1871 1934 3188 4158
Size: 1871 63 1254 970
Fragments arranged by size:
1871 1254 970 63
BtsI GCAGTG_nn'
Cuts at: 0 560 2381 4158
Size: 560 1821 1777
Fragments arranged by size:
1821 1777 560
ClaI AT'CG_AT
Cuts at: 0 2637 2765 2786 4158
Size: 2637 128 21 1372
Fragments arranged by size:
2637 1372 128 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 24 59 2724 2759 4158
Size: 24 35 2665 35 1399
Fragments arranged by size:
2665 1399 35 35 24
DraI TTT'AAA
Cuts at: 0 2960 4158
Size: 2960 1198
DraIII CAC_nnn'GTG
Cuts at: 0 1683 4158
Size: 1683 2475
EaeI y'GGCC_r
Cuts at: 0 445 3476 4158
Size: 445 3031 682
Fragments arranged by size:
3031 682 445
EarI CTCTTCn'nnn_
Cuts at: 0 63 344 743 1244 2672 3101 3453 3596
Size: 63 281 399 501 1428 429 352 143
Cuts at: 3596 4158
Size: 562
Fragments arranged by size:
1428 562 501 429 399 352 281 143
63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2307 4158
Size: 2307 1851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 183 277 853 2332 2522 3034 3125
Size: 144 39 94 576 1479 190 512 91
Cuts at: 3125 3740 4048 4158
Size: 615 308 110
Fragments arranged by size:
1479 615 576 512 308 190 144 110
94 91 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1243 2708 4158
Size: 1243 1465 1450
Fragments arranged by size:
1465 1450 1243
EcoO109I rG'GnC_Cy
Cuts at: 0 3563 4158
Size: 3563 595
EcoRI G'AATT_C
Cuts at: 0 797 897 4158
Size: 797 100 3261
Fragments arranged by size:
3261 797 100
EcoRV GAT'ATC
Cuts at: 0 3638 4158
Size: 3638 520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 233 307 4158
Size: 233 74 3851
Fragments arranged by size:
3851 233 74
HaeII r_GCGC'y
Cuts at: 0 216 4158
Size: 216 3942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 1237 1269 1609 1641 1848 1880
Size: 361 32 844 32 340 32 207 32
Cuts at: 1880 2047 2061 2079 2093 2823 2855 3566 3598
Size: 167 14 18 14 730 32 711 32
Cuts at: 3598 3863 3895 4106 4138 4158
Size: 265 32 211 32 20
Fragments arranged by size:
844 730 711 361 340 265 211 207
167 32 32 32 32 32 32 32
32 20 18 14 14
HincII GTy'rAC
Cuts at: 0 708 4158
Size: 708 3450
HindIII A'AGCT_T
Cuts at: 0 717 2278 4158
Size: 717 1561 1880
Fragments arranged by size:
1880 1561 717
KasI G'GCGC_C
Cuts at: 0 212 4158
Size: 212 3946
MfeI C'AATT_G
Cuts at: 0 2204 4133 4158
Size: 2204 1929 25
Fragments arranged by size:
2204 1929 25
MluI A'CGCG_T
Cuts at: 0 1833 4158
Size: 1833 2325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1235 3967 4158
Size: 972 263 2732 191
Fragments arranged by size:
2732 972 263 191
MscI TGG'CCA
Cuts at: 0 447 3478 4158
Size: 447 3031 680
Fragments arranged by size:
3031 680 447
MslI CAynn'nnrTG
Cuts at: 0 104 1028 1939 4135 4158
Size: 104 924 911 2196 23
Fragments arranged by size:
2196 924 911 104 23
MspA1I CmG'CkG
Cuts at: 0 451 3003 3236 3748 3912 4158
Size: 451 2552 233 512 164 246
Fragments arranged by size:
2552 512 451 246 233 164
NarI GG'CG_CC
Cuts at: 0 213 4158
Size: 213 3945
NdeI CA'TA_TG
Cuts at: 0 305 4158
Size: 305 3853
NsiI A_TGCA'T
Cuts at: 0 1047 4158
Size: 1047 3111
NspI r_CATG'y
Cuts at: 0 1546 4158
Size: 1546 2612
PasI CC'CwG_GG
Cuts at: 0 3299 4158
Size: 3299 859
PflMI CCAn_nnn'nTGG
Cuts at: 0 4017 4158
Size: 4017 141
PfoI T'CCnGG_A
Cuts at: 0 2348 4064 4117 4158
Size: 2348 1716 53 41
Fragments arranged by size:
2348 1716 53 41
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 452 484 4158
Size: 452 32 3674
Fragments arranged by size:
3674 452 32
PsiI TTA'TAA
Cuts at: 0 2728 3428 4158
Size: 2728 700 730
Fragments arranged by size:
2728 730 700
PspOMI G'GGCC_C
Cuts at: 0 3563 4158
Size: 3563 595
PspXI vC'TCGA_Gb
Cuts at: 0 4109 4158
Size: 4109 49
PstI C_TGCA'G
Cuts at: 0 2712 3439 3470 3892 4158
Size: 2712 727 31 422 266
Fragments arranged by size:
2712 727 422 266 31
PvuII CAG'CTG
Cuts at: 0 451 3003 3748 4158
Size: 451 2552 745 410
Fragments arranged by size:
2552 745 451 410
SapI GCTCTTCn'nnn_
Cuts at: 0 344 2672 4158
Size: 344 2328 1486
Fragments arranged by size:
2328 1486 344
SbfI CC_TGCA'GG
Cuts at: 0 2712 4158
Size: 2712 1446
ScaI AGT'ACT
Cuts at: 0 3717 4158
Size: 3717 441
SexAI A'CCwGG_T
Cuts at: 0 1679 2160 4158
Size: 1679 481 1998
Fragments arranged by size:
1998 1679 481
SfcI C'TryA_G
Cuts at: 0 2599 2708 3435 3466 3888 4158
Size: 2599 109 727 31 422 270
Fragments arranged by size:
2599 727 422 270 109 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2894 4158
Size: 2894 1264
SfoI GGC'GCC
Cuts at: 0 214 4158
Size: 214 3944
SmlI C'TyrA_G
Cuts at: 0 763 1495 2861 4109 4158
Size: 763 732 1366 1248 49
Fragments arranged by size:
1366 1248 763 732 49
SpeI A'CTAG_T
Cuts at: 0 3865 4158
Size: 3865 293
StuI AGG'CCT
Cuts at: 0 157 389 1189 2497 4158
Size: 157 232 800 1308 1661
Fragments arranged by size:
1661 1308 800 232 157
StyI C'CwwG_G
Cuts at: 0 3567 4158
Size: 3567 591
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2666 4158
Size: 2666 1492
TatI w'GTAC_w
Cuts at: 0 888 1539 3400 3715 3783 4158
Size: 888 651 1861 315 68 375
Fragments arranged by size:
1861 888 651 375 315 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1713 1745 2989 3021 3920 3952 4158
Size: 1713 32 1244 32 899 32 206
Fragments arranged by size:
1713 1244 899 206 32 32 32
XbaI T'CTAG_A
Cuts at: 0 58 2841 4158
Size: 58 2783 1317
Fragments arranged by size:
2783 1317 58
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2829 3574 4158
Size: 2829 745 584
Fragments arranged by size:
2829 745 584
XhoI C'TCGA_G
Cuts at: 0 4109 4158
Size: 4109 49
XmnI GAAnn'nnTTC
Cuts at: 0 797 1655 4158
Size: 797 858 2503
Fragments arranged by size:
2503 858 797
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlwNI ApaI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KasI MfeI MluI MmeI MscI MslI MspA1I
NarI NdeI NsiI NspI PasI PflMI PfoI PpiI
PsiI PspOMI PspXI PstI PvuII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmlI SpeI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvrII BcgI BmgBI BmtI
BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI DrdI
EagI EcoICRI FseI FspI FspAI HpaI KpnI NaeI
NcoI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpuMI PshAI PsrI PvuI RsrII SacI SacII
SalI SanDI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI Tth111I XmaI ZraI