################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbsI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI DraIII DrdI Eco47III Eco81I EcoNI EcoRV EcoT22I FbaI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AhdI 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 156 GGGCCC GGGCC^C Asp700I 841 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 505 CACGTG CAC^GTG BcgI 392 GCATGGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1044 GTATCC GTATCC(6/5) BpmI 1173 CTCCAG CTGGAG(16/14) BsaAI 505 CACGTG [CT]AC^GT[GA] BsaWI 140 TCCGGT [AT]^CCGG[AT] BspMI 1130 ACCTGC ACCTGC(4/8) BstAPI 1017 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1117 GGTGACC G^GT[ACGT]ACC BstPI 1117 GGTGACC G^GT[ACGT]ACC BstXI 1291 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 257 AGATCC [GA]^GATC[CT] CpoI 142 CGGTCCG CG^G[AT]CCG DraI 1546 TTTAAA TTT^AAA Eam1105I 997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1117 GGTGACC G^GT[ACGT]ACC EcoRI 778 GAATTC G^AATTC FseI 350 GGCCGGCC GGCCGG^CC HgaI 880 GACGC GACGC(5/10) HincII 1264 GTCAAC GT[CT]^[GA]AC HindII 1264 GTCAAC GT[CT]^[GA]AC MflI 257 AGATCC [GA]^GATC[CT] NaeI 351 GCCGGC GCC^GGC NcoI 1574 CCATGG C^CATGG NgoMIV 351 GCCGGC G^CCGGC NotI 383 GCGGCCGC GC^GGCCGC NspI 99 GCATGT [GA]CATG^[CT] PflMI 312 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 505 CACGTG CAC^GTG PmlI 505 CACGTG CAC^GTG PpuMI 1361 AGGACCT [GA]G^G[AT]CC[CT] PvuII 270 CAGCTG CAG^CTG RsrII 142 CGGTCCG CG^G[AT]CCG SacI 1579 GAGCTC GAGCT^C ScaI 743 AGTACT AGT^ACT SexAI 1039 ACCAGGT A^CC[AT]GGT Van91I 312 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 257 AGATCC [GA]^GATC[CT] XmnI 841 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AfaI 744 GTAC GT^AC 958 GTAC AspHI 415 GAGCAC G[AT]GC[AT]^C 1579 GAGCTC AviII 455 TGCGCA TGC^GCA 761 TGCGCA BanI 562 GGCGCC G^G[CT][GA]CC 985 GGCGCC BbeI 562 GGCGCC GGCGC^C 985 GGCGCC BbvCI 12 CCTCAGC CCTCAGC(-5/-2) 117 GCTGAGG BlpI 510 GCTGAGC GC^T[ACGT]AGC 672 GCTCAGC Bpu1102I 510 GCTGAGC GC^T[ACGT]AGC 672 GCTCAGC BseRI 1027 CTCCTC GAGGAG(10/8) 1030 CTCCTC BsiHKAI 415 GAGCAC G[AT]GC[AT]^C 1579 GAGCTC BspHI 611 TCATGA T^CATGA 1284 TCATGA CelII 510 GCTGAGC GC^T[ACGT]AGC 672 GCTCAGC DraII 314 AGGCCCT [GA]G^G[ACGT]CC[CT] 1361 AGGACCT DsaI 599 CCGTGG C^C[GA][CT]GG 1574 CCATGG EcoO109I 314 AGGCCCT [GA]G^G[ACGT]CC[CT] 1361 AGGACCT FspI 455 TGCGCA TGC^GCA 761 TGCGCA KasI 562 GGCGCC G^GCGCC 985 GGCGCC MseI 1532 TTAA T^TAA 1547 TTAA MslI 279 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 925 CACGGTGGTG NarI 562 GGCGCC GG^CGCC 985 GGCGCC PaeR7I 496 CTCGAG C^TCGAG 1061 CTCGAG PstI 739 CTGCAG CTGCA^G 1015 CTGCAG RcaI 611 TCATGA T^CATGA 1284 TCATGA RsaI 744 GTAC GT^AC 958 GTAC SfoI 562 GGCGCC GGC^GCC 985 GGCGCC SmaI 153 CCCGGG CCC^GGG 853 CCCGGG TfiI 1281 GAATC G^A[AT]TC 1331 GATTC Tru9I 1532 TTAA T^TAA 1547 TTAA XcmI 92 CCACTGTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1244 CCAAGGACACTGTGG XhoI 496 CTCGAG C^TCGAG 1061 CTCGAG XmaCI 153 CCCGGG C^CCGGG 853 CCCGGG XmaI 153 CCCGGG C^CCGGG 853 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwI AlwNI ApoI AvaI AvaII BalI BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EagI EarI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I