(Circular) (Six-base) MAPSORT of: pFN21AE6759.seq Check: 8514 from: 1 to: 6334 pFN21AE6759 6334 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2791 4397 5781 6049 1632 Size: 1159 1606 1384 268 1917 Fragments arranged by size: 1917 1606 1384 1159 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 3007 5063 3007 Size: 2056 4278 AleI CACnn'nnGTG Cuts at: 1166 2934 1166 Size: 1768 4566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2119 2323 3028 4077 5649 2119 Size: 204 705 1049 1572 2804 Fragments arranged by size: 2804 1572 1049 705 204 ApaI G_GGCC'C Cuts at: 2165 2165 Size: 6334 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 2783 3514 3607 3643 3869 4021 6130 1493 Size: 1290 731 93 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 1290 731 226 152 93 36 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2158 2501 2581 2660 2858 3066 3659 Size: 73 215 343 80 79 198 208 593 Cuts at: 3659 1870 Size: 4545 Fragments arranged by size: 4545 593 343 215 208 198 80 79 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2567 2990 3655 5110 618 Size: 325 217 627 780 423 665 1455 1842 Fragments arranged by size: 1842 1455 780 665 627 423 325 217 BanII G_rGCy'C Cuts at: 1391 2165 2339 3588 3653 1391 Size: 774 174 1249 65 4072 Fragments arranged by size: 4072 1249 774 174 65 BbeI G_GCGC'C Cuts at: 2571 2994 2571 Size: 423 5911 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5363 BbvCI CC'TCA_GC Cuts at: 2018 2123 2018 Size: 105 6229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2386 2420 4525 4559 1903 Size: 34 449 34 2105 34 3678 Fragments arranged by size: 3678 2105 449 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3060 4226 5436 3060 Size: 1166 1210 3958 Fragments arranged by size: 3958 1210 1166 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 2516 2678 3751 2516 Size: 162 1073 5099 Fragments arranged by size: 5099 1073 162 Bme1580I G_kGCm'C Cuts at: 1790 2165 4395 5551 1790 Size: 375 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 375 BmgBI CAC'GTC Cuts at: 1318 2731 1318 Size: 1413 4921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3290 4065 5023 Size: 416 53 83 399 24 1873 775 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1873 1753 958 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3163 4994 1239 Size: 1924 1831 2579 Fragments arranged by size: 2579 1924 1831 Bpu10I CC'TnA_GC Cuts at: 2018 2123 2018 Size: 105 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2854 4459 5324 5622 2854 Size: 1605 865 298 3566 Fragments arranged by size: 3566 1605 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2512 4125 493 Size: 711 1308 1613 2702 Fragments arranged by size: 2702 1613 1308 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2568 2886 2991 4141 Size: 53 83 186 936 1035 318 105 1150 Cuts at: 4141 4523 5158 275 Size: 382 635 1451 Fragments arranged by size: 1451 1150 1035 936 635 382 318 186 105 83 53 BsaWI w'CCGG_w Cuts at: 2145 4767 5165 5439 5586 5997 2145 Size: 2622 398 274 147 411 2482 Fragments arranged by size: 2622 2482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3091 3121 1178 Size: 30 165 30 1688 30 4391 Fragments arranged by size: 4391 1688 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3023 3026 1186 Size: 1837 3 4494 Fragments arranged by size: 4494 1837 3 BseYI C'CCAG_C Cuts at: 5537 6033 5537 Size: 496 5838 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 2453 3080 3092 1163 Size: 573 612 105 627 12 4405 Fragments arranged by size: 4405 627 612 573 105 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2353 2357 2392 2919 3784 4545 Size: 275 186 352 4 35 527 865 761 Cuts at: 4545 5573 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 865 761 527 352 275 186 35 4 BsiHKAI G_wGCw'C Cuts at: 2424 3588 4395 4480 5551 2424 Size: 1164 807 85 1071 3207 Fragments arranged by size: 3207 1164 1071 807 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2339 2424 3588 3653 4395 4480 Size: 399 375 174 85 1164 65 742 85 Cuts at: 4480 5551 1391 Size: 1071 2174 Fragments arranged by size: 2174 1164 1071 742 399 375 174 85 85 65 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2616 3289 4223 5953 2616 Size: 673 934 1730 2997 Fragments arranged by size: 2997 1730 934 673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3144 3689 844 Size: 2300 545 3489 Fragments arranged by size: 3489 2300 545 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2352 2356 2965 4978 5165 1700 Size: 110 542 4 609 2013 187 2869 Fragments arranged by size: 2869 2013 609 542 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 2305 2440 2525 2940 1925 Size: 380 135 85 415 5319 Fragments arranged by size: 5319 415 380 135 85 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 3028 794 Size: 2234 4100 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 3122 1184 Size: 1938 4396 BstXI CCAn_nnnn'nTGG Cuts at: 3303 3303 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2262 3664 4031 4416 4433 5885 5971 5983 Size: 290 1402 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1402 385 367 346 290 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2604 3579 513 Size: 552 939 600 975 3268 Fragments arranged by size: 3268 975 939 600 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3029 3213 3850 4644 4664 2839 Size: 190 184 637 794 20 4509 Fragments arranged by size: 4509 794 637 190 184 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3553 3638 3553 Size: 85 6249 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2094 2174 Size: 54 1425 50 275 18 206 58 80 Cuts at: 2174 2354 2389 2407 2601 2607 2916 2967 3781 Size: 180 35 18 194 6 309 51 814 Cuts at: 3781 4670 6299 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 814 309 275 206 194 180 80 58 54 51 50 43 35 18 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2354 2389 2916 3781 1537 Size: 275 542 35 527 865 4090 Fragments arranged by size: 4090 865 542 527 275 35 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 3044 3455 4264 1353 Size: 1332 359 411 809 3423 Fragments arranged by size: 3423 1332 809 411 359 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 4921 5295 5441 387 Size: 1064 138 666 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 666 374 146 138 EcoICRI GAG'CTC Cuts at: 3586 3586 Size: 6334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2791 3163 4397 4994 5781 6049 1239 Size: 393 1159 372 1234 597 787 268 1524 Fragments arranged by size: 1524 1234 1159 787 597 393 372 268 EcoO109I rG'GnC_Cy Cuts at: 2320 3367 2320 Size: 1047 5287 EcoRI G'AATT_C Cuts at: 2783 3643 4021 2783 Size: 860 378 5096 Fragments arranged by size: 5096 860 378 FseI GG_CCGG'CC Cuts at: 2360 2360 Size: 6334 FspI TGC'GCA Cuts at: 2462 2768 4840 2462 Size: 306 2072 3956 Fragments arranged by size: 3956 2072 306 FspAI rTGC'GCAy Cuts at: 2768 2768 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2245 2543 2571 2994 5481 1678 Size: 567 298 28 423 2487 2531 Fragments arranged by size: 2531 2487 567 423 298 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2993 3025 3091 3123 4975 Size: 32 135 32 1421 32 66 32 1852 Cuts at: 4975 5007 5049 5081 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1852 1421 135 66 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3271 3678 3924 669 Size: 988 281 1333 407 246 3079 Fragments arranged by size: 3079 1333 988 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2567 2990 2567 Size: 423 5911 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2176 2609 10 Size: 54 1425 343 264 80 433 3735 Fragments arranged by size: 3735 1425 433 343 264 80 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2934 4292 4651 4810 518 Size: 648 1122 646 1358 359 159 2042 Fragments arranged by size: 2042 1358 1122 648 646 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2116 2277 2437 2590 3750 4427 5575 Size: 444 66 161 160 153 1160 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1160 1148 677 444 245 161 160 153 66 NaeI GCC'GGC Cuts at: 2358 2358 Size: 6334 NarI GG'CG_CC Cuts at: 2568 2991 2568 Size: 423 5911 NcoI C'CATG_G Cuts at: 513 1065 2004 3579 513 Size: 552 939 1575 3268 Fragments arranged by size: 3268 1575 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2356 2356 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 2389 3781 2389 Size: 1392 4942 NspI r_CATG'y Cuts at: 1132 1229 2108 3692 5237 1132 Size: 97 879 1584 1545 2229 Fragments arranged by size: 2229 1584 1545 879 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 5119 PfoI T'CCnGG_A Cuts at: 2820 2820 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PmlI CAC'GTG Cuts at: 2512 2512 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3367 3367 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2161 2161 Size: 6334 PspXI vC'TCGA_Gb Cuts at: 2501 3066 2501 Size: 565 5769 PstI C_TGCA'G Cuts at: 830 2748 3024 3686 830 Size: 1918 276 662 3478 Fragments arranged by size: 3478 1918 662 276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2277 2277 Size: 6334 RsrII CG'GwC_CG Cuts at: 2148 2148 Size: 6334 SacI G_AGCT'C Cuts at: 3588 3588 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 2750 4582 6294 2750 Size: 1832 1712 2790 Fragments arranged by size: 2790 1832 1712 SexAI A'CCwGG_T Cuts at: 3044 3044 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 2644 2744 3020 3682 4817 5498 5689 Size: 219 1599 100 276 662 1135 681 191 Cuts at: 5689 826 Size: 1471 Fragments arranged by size: 1599 1471 1135 681 662 276 219 191 100 SfoI GGC'GCC Cuts at: 2569 2992 2569 Size: 423 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 2160 2860 3661 1872 Size: 288 700 801 4545 Fragments arranged by size: 4545 801 700 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 2833 3066 4438 5339 5601 Size: 197 926 558 332 233 1372 901 262 Cuts at: 5601 820 Size: 1553 Fragments arranged by size: 1553 1372 926 901 558 332 262 233 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2004 2033 3249 3579 3618 3773 513 Size: 552 939 29 1216 330 39 155 3074 Fragments arranged by size: 3074 1216 939 552 330 155 39 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3110 4361 4378 4531 4716 5161 325 Size: 1255 1530 1251 17 153 185 445 1498 Fragments arranged by size: 1530 1498 1255 1251 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 4580 6292 Size: 274 80 33 51 1443 771 1832 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1832 1712 1443 771 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3034 3066 6294 6326 3034 Size: 32 3228 32 3042 Fragments arranged by size: 3228 3042 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2104 3256 2104 Size: 1152 5182 XhoI C'TCGA_G Cuts at: 1943 2501 3066 1943 Size: 558 565 5211 Fragments arranged by size: 5211 565 558 XmaI C'CCGG_G Cuts at: 1870 2158 2858 3659 1870 Size: 288 700 801 4545 Fragments arranged by size: 4545 801 700 288 XmnI GAAnn'nnTTC Cuts at: 2850 4463 2850 Size: 1613 4721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI NruI NsiI PacI PsrI SacII SanDI SapI SfiI SrfI SwaI