(Linear) (Six-base) MAPSORT of: KIEE6759.seq Check: 455 from: 1 to: 1629 KIEE6759.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 786 1629 Size: 786 843 AhdI GACnn_n'nnGTC Cuts at: 0 1002 1629 Size: 1002 627 AleI CACnn'nnGTG Cuts at: 0 929 1629 Size: 929 700 AlwNI CAG_nnn'CTG Cuts at: 0 114 318 1023 1629 Size: 114 204 705 606 Fragments arranged by size: 705 606 204 114 ApaI G_GGCC'C Cuts at: 0 160 1629 Size: 160 1469 ApoI r'AATT_y Cuts at: 0 778 1509 1602 1629 Size: 778 731 93 27 Fragments arranged by size: 778 731 93 27 AvaI C'yCGr_G Cuts at: 0 153 496 576 655 853 1061 1629 Size: 153 343 80 79 198 208 568 Fragments arranged by size: 568 343 208 198 153 80 79 BanI G'GyrC_C Cuts at: 0 562 985 1629 Size: 562 423 644 Fragments arranged by size: 644 562 423 BanII G_rGCy'C Cuts at: 0 160 334 1583 1629 Size: 160 174 1249 46 Fragments arranged by size: 1249 174 160 46 BbeI G_GCGC'C Cuts at: 0 566 989 1629 Size: 566 423 640 Fragments arranged by size: 640 566 423 BbvCI CC'TCA_GC Cuts at: 0 13 118 1629 Size: 13 105 1511 Fragments arranged by size: 1511 105 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 381 415 1629 Size: 381 34 1214 Fragments arranged by size: 1214 381 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1055 1629 Size: 1055 574 BlpI GC'TnA_GC Cuts at: 0 511 673 1629 Size: 511 162 956 Fragments arranged by size: 956 511 162 Bme1580I G_kGCm'C Cuts at: 0 160 1629 Size: 160 1469 BmgBI CAC'GTC Cuts at: 0 726 1629 Size: 726 903 BmrI ACTGGGnnnn_n' Cuts at: 0 1285 1629 Size: 1285 344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1158 1629 Size: 1158 471 Bpu10I CC'TnA_GC Cuts at: 0 13 118 1629 Size: 13 105 1511 Fragments arranged by size: 1511 105 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 849 1629 Size: 849 780 BsaAI yAC'GTr Cuts at: 0 507 1629 Size: 507 1122 BsaHI Gr'CG_yC Cuts at: 0 563 881 986 1629 Size: 563 318 105 643 Fragments arranged by size: 643 563 318 105 BsaWI w'CCGG_w Cuts at: 0 140 1629 Size: 140 1489 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1086 1116 1629 Size: 1086 30 513 Fragments arranged by size: 1086 513 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1018 1021 1629 Size: 1018 3 608 Fragments arranged by size: 1018 608 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 448 1075 1087 1629 Size: 343 105 627 12 542 Fragments arranged by size: 627 542 343 105 12 BsiEI CG_ry'CG Cuts at: 0 348 352 387 914 1629 Size: 348 4 35 527 715 Fragments arranged by size: 715 527 348 35 4 BsiHKAI G_wGCw'C Cuts at: 0 419 1583 1629 Size: 419 1164 46 Fragments arranged by size: 1164 419 46 Bsp1286I G_dGCh'C Cuts at: 0 160 334 419 1583 1629 Size: 160 174 85 1164 46 Fragments arranged by size: 1164 174 160 85 46 BspHI T'CATG_A Cuts at: 0 611 1284 1629 Size: 611 673 345 Fragments arranged by size: 673 611 345 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1139 1629 Size: 1139 490 BsrFI r'CCGG_y Cuts at: 0 347 351 960 1629 Size: 347 4 609 669 Fragments arranged by size: 669 609 347 4 BssHII G'CGCG_C Cuts at: 0 300 435 520 935 1629 Size: 300 135 85 415 694 Fragments arranged by size: 694 415 300 135 85 BstAPI GCAn_nnn'nTGC Cuts at: 0 1023 1629 Size: 1023 606 BstEII G'GTnAC_C Cuts at: 0 1117 1629 Size: 1117 512 BstXI CCAn_nnnn'nTGG Cuts at: 0 1298 1629 Size: 1298 331 BstYI r'GATC_y Cuts at: 0 257 1629 Size: 257 1372 BtgI C'CryG_G Cuts at: 0 599 1574 1629 Size: 599 975 55 Fragments arranged by size: 975 599 55 BtsI GCAGTG_nn' Cuts at: 0 834 1024 1208 1629 Size: 834 190 184 421 Fragments arranged by size: 834 421 190 184 DraI TTT'AAA Cuts at: 0 1548 1629 Size: 1548 81 EaeI y'GGCC_r Cuts at: 0 31 89 169 349 384 402 596 602 Size: 31 58 80 180 35 18 194 6 Cuts at: 602 911 962 1629 Size: 309 51 667 Fragments arranged by size: 667 309 194 180 80 58 51 35 31 18 6 EagI C'GGCC_G Cuts at: 0 349 384 911 1629 Size: 349 35 527 718 Fragments arranged by size: 718 527 349 35 EarI CTCTTCn'nnn_ Cuts at: 0 680 1039 1450 1629 Size: 680 359 411 179 Fragments arranged by size: 680 411 359 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 250 1629 Size: 250 1379 EcoICRI GAG'CTC Cuts at: 0 1581 1629 Size: 1581 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 786 1158 1629 Size: 786 372 471 Fragments arranged by size: 786 471 372 EcoO109I rG'GnC_Cy Cuts at: 0 315 1362 1629 Size: 315 1047 267 Fragments arranged by size: 1047 315 267 EcoRI G'AATT_C Cuts at: 0 778 1629 Size: 778 851 FseI GG_CCGG'CC Cuts at: 0 355 1629 Size: 355 1274 FspI TGC'GCA Cuts at: 0 457 763 1629 Size: 457 306 866 Fragments arranged by size: 866 457 306 FspAI rTGC'GCAy Cuts at: 0 763 1629 Size: 763 866 HaeII r_GCGC'y Cuts at: 0 240 538 566 989 1629 Size: 240 298 28 423 640 Fragments arranged by size: 640 423 298 240 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 988 1020 1086 1118 1629 Size: 988 32 66 32 511 Fragments arranged by size: 988 511 66 32 32 HincII GTy'rAC Cuts at: 0 1266 1629 Size: 1266 363 KasI G'GCGC_C Cuts at: 0 562 985 1629 Size: 562 423 644 Fragments arranged by size: 644 562 423 MscI TGG'CCA Cuts at: 0 91 171 604 1629 Size: 91 80 433 1025 Fragments arranged by size: 1025 433 91 80 MslI CAynn'nnrTG Cuts at: 0 283 929 1629 Size: 283 646 700 Fragments arranged by size: 700 646 283 MspA1I CmG'CkG Cuts at: 0 45 111 272 432 585 1629 Size: 45 66 161 160 153 1044 Fragments arranged by size: 1044 161 160 153 66 45 NaeI GCC'GGC Cuts at: 0 353 1629 Size: 353 1276 NarI GG'CG_CC Cuts at: 0 563 986 1629 Size: 563 423 643 Fragments arranged by size: 643 563 423 NcoI C'CATG_G Cuts at: 0 1574 1629 Size: 1574 55 NgoMIV G'CCGG_C Cuts at: 0 351 1629 Size: 351 1278 NotI GC'GGCC_GC Cuts at: 0 384 1629 Size: 384 1245 NspI r_CATG'y Cuts at: 0 103 1629 Size: 103 1526 PflMI CCAn_nnn'nTGG Cuts at: 0 318 1629 Size: 318 1311 PfoI T'CCnGG_A Cuts at: 0 815 1629 Size: 815 814 PmlI CAC'GTG Cuts at: 0 507 1629 Size: 507 1122 PpuMI rG'GwC_Cy Cuts at: 0 1362 1629 Size: 1362 267 PspOMI G'GGCC_C Cuts at: 0 156 1629 Size: 156 1473 PspXI vC'TCGA_Gb Cuts at: 0 496 1061 1629 Size: 496 565 568 Fragments arranged by size: 568 565 496 PstI C_TGCA'G Cuts at: 0 743 1019 1629 Size: 743 276 610 Fragments arranged by size: 743 610 276 PvuII CAG'CTG Cuts at: 0 272 1629 Size: 272 1357 RsrII CG'GwC_CG Cuts at: 0 143 1629 Size: 143 1486 SacI G_AGCT'C Cuts at: 0 1583 1629 Size: 1583 46 ScaI AGT'ACT Cuts at: 0 745 1629 Size: 745 884 SexAI A'CCwGG_T Cuts at: 0 1039 1629 Size: 1039 590 SfcI C'TryA_G Cuts at: 0 639 739 1015 1629 Size: 639 100 276 614 Fragments arranged by size: 639 614 276 100 SfoI GGC'GCC Cuts at: 0 564 987 1629 Size: 564 423 642 Fragments arranged by size: 642 564 423 SmaI CCC'GGG Cuts at: 0 155 855 1629 Size: 155 700 774 Fragments arranged by size: 774 700 155 SmlI C'TyrA_G Cuts at: 0 496 828 1061 1629 Size: 496 332 233 568 Fragments arranged by size: 568 496 332 233 StyI C'CwwG_G Cuts at: 0 28 1244 1574 1613 1629 Size: 28 1216 330 39 16 Fragments arranged by size: 1216 330 39 28 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1105 1629 Size: 1105 524 TatI w'GTAC_w Cuts at: 0 743 1629 Size: 743 886 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1029 1061 1629 Size: 1029 32 568 Fragments arranged by size: 1029 568 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 99 1251 1629 Size: 99 1152 378 Fragments arranged by size: 1152 378 99 XhoI C'TCGA_G Cuts at: 0 496 1061 1629 Size: 496 565 568 Fragments arranged by size: 568 565 496 XmaI C'CCGG_G Cuts at: 0 153 853 1629 Size: 153 700 776 Fragments arranged by size: 776 700 153 XmnI GAAnn'nnTTC Cuts at: 0 845 1629 Size: 845 784 Enzymes that do cut: AcuI AhdI AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII SacI ScaI SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BclI BfrBI BglI BglII BmtI BplI BsaI BsaBI BseYI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EcoNI EcoRV FalI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PsrI PvuI SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI ZraI