(Circular) (Six-base) MAPSORT of: pFN21AE6368.seq Check: 1914 from: 1 to: 5788 pFN21AE6368 5788 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2055 2055 Size: 5788 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 2352 3109 2352 Size: 757 5031 AclI AA'CG_TT Cuts at: 1563 2771 3915 4288 1563 Size: 1208 1144 373 3063 Fragments arranged by size: 3063 1208 1144 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3003 3851 5235 5503 1632 Size: 650 721 848 1384 268 1917 Fragments arranged by size: 1917 1384 848 721 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 2355 4619 2355 Size: 2264 3524 AhdI GACnn_n'nnGTC Cuts at: 2408 4517 2408 Size: 2109 3679 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2149 2286 3016 3097 3323 3475 5584 1493 Size: 656 137 730 81 226 152 2109 1697 Fragments arranged by size: 2109 1697 730 656 226 152 137 81 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2239 3113 1870 Size: 73 296 874 4545 Fragments arranged by size: 4545 874 296 73 BamHI G'GATC_C Cuts at: 2243 3118 3485 2243 Size: 875 367 4546 Fragments arranged by size: 4546 875 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2180 2352 2705 3109 Size: 325 217 627 249 144 172 353 404 Cuts at: 3109 4564 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 404 353 325 249 217 172 144 BanII G_rGCy'C Cuts at: 1391 2761 3107 1391 Size: 1370 346 4072 Fragments arranged by size: 4072 1370 346 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 928 Size: 971 124 4693 Fragments arranged by size: 4693 971 124 BbvCI CC'TCA_GC Cuts at: 2104 2104 Size: 5788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2039 3849 5005 1790 Size: 249 1810 1156 2573 Fragments arranged by size: 2573 1810 1156 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2317 3519 4477 Size: 416 53 83 399 24 900 1202 958 Cuts at: 4477 442 Size: 1753 Fragments arranged by size: 1753 1202 958 900 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 2715 4448 1239 Size: 1328 148 1733 2579 Fragments arranged by size: 2579 1733 1328 148 Bpu10I CC'TnA_GC Cuts at: 2104 2104 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2353 2616 2840 3913 4778 5076 2353 Size: 263 224 1073 865 298 3065 Fragments arranged by size: 3065 1073 865 298 263 224 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2631 2655 4451 882 Size: 617 1132 24 1796 2219 Fragments arranged by size: 2219 1796 1132 617 24 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2355 4221 4619 4893 5040 5451 2355 Size: 1866 398 274 147 411 2692 Fragments arranged by size: 2692 1866 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 2864 2894 1178 Size: 30 165 30 1011 30 420 30 4072 Fragments arranged by size: 4072 1011 420 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2619 1186 Size: 946 487 4355 Fragments arranged by size: 4355 946 487 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2761 3849 3934 5005 2761 Size: 1088 85 1071 3544 Fragments arranged by size: 3544 1088 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 BsmBI CGTCTCn'nnnn_ Cuts at: 2976 2976 Size: 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2761 3107 3849 3934 5005 1391 Size: 399 249 722 346 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 722 399 346 249 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 2839 3677 5407 2839 Size: 838 1730 3220 Fragments arranged by size: 3220 1730 838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 3143 844 Size: 1211 1088 3489 Fragments arranged by size: 3489 1211 1088 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2219 2505 4283 4457 65 Size: 1183 971 286 1778 174 1396 Fragments arranged by size: 1778 1396 1183 971 286 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2022 2355 4432 4619 1700 Size: 110 212 333 2077 187 2869 Fragments arranged by size: 2869 2077 333 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 2517 3848 4860 2517 Size: 1331 1012 3445 Fragments arranged by size: 3445 1331 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2278 794 Size: 1484 4304 BstBI TT'CG_AA Cuts at: 2808 4592 2808 Size: 1784 4004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2720 2720 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2243 3118 3485 3870 3887 5339 5425 5437 Size: 271 875 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 875 385 367 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3304 Fragments arranged by size: 3304 993 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2391 3304 4098 4118 2391 Size: 913 794 20 4061 Fragments arranged by size: 4061 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2111 3235 Size: 54 1425 50 275 18 231 50 1124 Cuts at: 3235 4124 5753 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1124 889 275 231 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 2601 2935 3718 1353 Size: 813 435 334 783 3423 Fragments arranged by size: 3423 813 783 435 334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2028 4375 4749 4895 387 Size: 1064 138 439 2347 374 146 1280 Fragments arranged by size: 2347 1280 1064 439 374 146 138 EcoICRI GAG'CTC Cuts at: 2759 2759 Size: 5788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2567 2715 3003 3851 4448 5235 Size: 393 650 285 148 288 848 597 787 Cuts at: 5235 5503 1239 Size: 268 1524 Fragments arranged by size: 1524 848 787 650 597 393 288 285 268 148 EcoRI G'AATT_C Cuts at: 2149 2286 3097 3475 2149 Size: 137 811 378 4462 Fragments arranged by size: 4462 811 378 137 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2394 2426 2743 2775 2956 Size: 32 135 32 822 32 317 32 181 Cuts at: 2956 2988 4429 4461 4503 4535 1373 Size: 32 1441 32 42 32 2626 Fragments arranged by size: 2626 1441 822 317 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 2356 3113 2356 Size: 757 5031 MfeI C'AATT_G Cuts at: 2823 3387 2823 Size: 564 5224 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 3051 4902 5086 1305 Size: 1182 564 1851 184 2007 Fragments arranged by size: 2007 1851 1182 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2113 10 Size: 54 1425 343 281 3685 Fragments arranged by size: 3685 1425 343 281 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 4105 4264 518 Size: 648 2580 359 159 2042 Fragments arranged by size: 2580 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2587 3204 3881 5029 5274 1606 Size: 981 617 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 677 617 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3304 Fragments arranged by size: 3304 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 2073 1348 Size: 725 5063 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2587 2587 Size: 5788 SacI G_AGCT'C Cuts at: 2761 2761 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SapI GCTCTTCn'nnn_ Cuts at: 2166 2166 Size: 5788 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 3136 4271 4952 5143 826 Size: 219 2091 1135 681 191 1471 Fragments arranged by size: 2091 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 2022 1700 Size: 322 5466 SmaI CCC'GGG Cuts at: 1872 2241 3115 1872 Size: 369 874 4545 Fragments arranged by size: 4545 874 369 SmlI C'TyrA_G Cuts at: 820 1017 1943 2332 2631 2855 3892 4793 5055 Size: 197 926 389 299 224 1037 901 262 Cuts at: 5055 820 Size: 1553 Fragments arranged by size: 1553 1037 926 901 389 299 262 224 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 2997 3227 513 Size: 552 939 993 230 3074 Fragments arranged by size: 3074 993 939 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2703 3815 3832 3985 4170 4615 325 Size: 1255 1123 1112 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1123 1112 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2371 2403 2414 2446 5748 5780 2371 Size: 32 11 32 3302 32 2379 Fragments arranged by size: 3302 2379 32 32 32 11 Tth111I GACn'n_nGTC Cuts at: 1316 2309 1316 Size: 993 4795 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2239 3113 1870 Size: 369 874 4545 Fragments arranged by size: 4545 874 369 XmnI GAAnn'nnTTC Cuts at: 2814 3078 3917 2814 Size: 264 839 4685 Fragments arranged by size: 4685 839 264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI