################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV FbaI FseI FspI HindIII KpnI MluI MroI NcoI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SapI ScaI SexAI SfiI SfuI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 473 GTCGAC GT^[AC][GT]AC AccII 93 CGCG CG^CG AflIII 2215 ACGTGT A^C[GA][CT]GT ApaI 179 GGGCCC GGGCC^C AseI 1168 ATTAAT AT^TAAT AsnI 1168 ATTAAT AT^TAAT Asp700I 2257 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1500 GAGCAC G[AT]GC[AT]^C BalI 1292 TGGCCA TGG^CCA BamHI 152 GGATCC G^GATCC BbeI 49 GGCGCC GGCGC^C BciVI 2219 GTATCC GTATCC(6/5) BsaBI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 297 GAGGAG GAGGAG(10/8) BsiEI 95 CGGCCG CG[GA][CT]^CG BsiHKAI 1500 GAGCAC G[AT]GC[AT]^C BsmI 1946 GCATTC GAATGC(1/-1) BspDI 2259 ATCGAT AT^CGAT BsrBI 217 GAGCGG CCGCTC(-3/-3) BssSI 172 CACGAG CACGAG(-5/-1) BstUI 93 CGCG CG^CG CfoI 50 GCGC GCG^C ClaI 2259 ATCGAT AT^CGAT DraIII 1811 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 92 CCGCGG C^C[GA][CT]GG EagI 95 CGGCCG C^GGCCG EclXI 95 CGGCCG C^GGCCG Eco52I 95 CGGCCG C^GGCCG EcoNI 1690 CCTCTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2227 GAATTC G^AATTC EcoT14I 62 CCAAGG C^C[AT][AT]GG EcoT22I 1340 ATGCAT ATGCA^T HaeII 49 GGCGCC [GA]GCGC^[CT] HhaI 50 GCGC GCG^C HinP1I 50 GCGC G^CGC HpaI 669 GTTAAC GTT^AAC KasI 49 GGCGCC G^GCGCC KspI 92 CCGCGG CCGC^GG MamI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 794 CAATTG C^AATTG MluNI 1292 TGGCCA TGG^CCA MscI 1292 TGGCCA TGG^CCA MunI 794 CAATTG C^AATTG MvnI 93 CGCG CG^CG NarI 49 GGCGCC GG^CGCC NdeI 1885 CATATG CA^TATG NsiI 1340 ATGCAT ATGCA^T PflMI 2097 CCACTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 15 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 940 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1168 ATTAAT AT^TAAT PvuII 719 CAGCTG CAG^CTG SacII 92 CCGCGG CCGC^GG SalI 473 GTCGAC G^TCGAC SbfI 249 CCTGCAGG CCTGCA^GG SfoI 49 GGCGCC GGC^GCC SgrAI 51 CGCCGGCG C[GA]^CCGG[CT]G SmlI 994 CTCAAG C^T[CT][GA]AG Sse8387I 249 CCTGCAGG CCTGCA^GG StuI 1281 AGGCCT AGG^CCT StyI 62 CCAAGG C^C[AT][AT]GG Van91I 2097 CCACTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2257 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 22 GGCGTC G[GA]^CG[CT]C 49 GGCGCC AfaI 138 GTAC GT^AC 1604 GTAC AlwNI 626 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 674 CAGATTCTG BanI 49 GGCGCC G^G[CT][GA]CC 58 GGTGCC BsaHI 22 GGCGTC G[GA]^CG[CT]C 49 GGCGCC BsrFI 52 GCCGGC [GA]^CCGG[CT] 97 GCCGGC BstAPI 159 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 649 GCAATGATTGC Cfr10I 52 GCCGGC [GA]^CCGG[CT] 97 GCCGGC DraII 15 GGGTCCC [GA]G^G[ACGT]CC[CT] 1686 AGGCCCT EaeI 95 CGGCCG [CT]^GGCC[GA] 1292 TGGCCA EcoO109I 15 GGGTCCC [GA]G^G[ACGT]CC[CT] 1686 AGGCCCT Hin1I 22 GGCGTC G[GA]^CG[CT]C 49 GGCGCC HincII 473 GTCGAC GT[CT]^[GA]AC 669 GTTAAC HindII 473 GTCGAC GT[CT]^[GA]AC 669 GTTAAC MaeII 2190 ACGT A^CGT 2215 ACGT NaeI 52 GCCGGC GCC^GGC 97 GCCGGC NgoMIV 52 GCCGGC G^CCGGC 97 GCCGGC RsaI 138 GTAC GT^AC 1604 GTAC SmaI 45 CCCGGG CCC^GGG 147 CCCGGG XmaCI 45 CCCGGG C^CCGGG 147 CCCGGG XmaI 45 CCCGGG C^CCGGG 147 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RcaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII