Restriction map for
pFN21AB5794
(Circular) (Six-base) MAPSORT of: pFN21AB5794.seq Check: 3337 from: 1 to: 7045
pFN21AB5794 7045 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2479 4388 1937
Size: 542 1909 4594
Fragments arranged by size:
4594 1909 542
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 2658 2982 5108 6492 6760 1632
Size: 388 638 324 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 638 388 324 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 4220 5944 6921 1203
Size: 3017 1724 977 1327
Fragments arranged by size:
3017 1724 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3834 3866 3834
Size: 32 7013
AlwNI CAG_nnn'CTG
Cuts at: 2636 2684 4788 6360 2636
Size: 48 2104 1572 3321
Fragments arranged by size:
3321 2104 1572 48
ApaI G_GGCC'C
Cuts at: 2188 2188
Size: 7045
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2281 2708 3475 4070 4232 4354 4580 4732
Size: 788 427 767 595 162 122 226 152
Cuts at: 4732 6841 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 788 767 595 427 226 162
152 122
AseI AT'TA_AT
Cuts at: 160 1032 3174 5599 160
Size: 872 2142 2425 1606
Fragments arranged by size:
2425 2142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2075 2133 2152 3883 4370 1870
Size: 73 107 25 58 19 1731 487 4545
Fragments arranged by size:
4545 1731 487 107 73 58 25 19
BamHI G'GATC_C
Cuts at: 2157 4375 4742 2157
Size: 2218 367 4460
Fragments arranged by size:
4460 2218 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2063 4366 5821 618
Size: 325 217 627 267 9 2303 1455 1842
Fragments arranged by size:
2303 1842 1455 627 325 267 217 9
BanII G_rGCy'C
Cuts at: 1391 2076 2140 2188 2209 3409 3547 4364 1391
Size: 685 64 48 21 1200 138 817 4072
Fragments arranged by size:
4072 1200 817 685 138 64 48 21
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 2949 3362 4062 928
Size: 971 124 926 413 700 3911
Fragments arranged by size:
3911 971 926 700 413 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 4937 6147 4235
Size: 702 1210 5133
Fragments arranged by size:
5133 1210 702
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7045
BfrBI ATG'CAT
Cuts at: 3347 3347
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5656 136
Size: 107 122 71 631 605 3984 1525
Fragments arranged by size:
3984 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7040 7040
Size: 7045
BlpI GC'TnA_GC
Cuts at: 4462 4462
Size: 7045
Bme1580I G_kGCm'C
Cuts at: 1790 2188 5106 6262 1790
Size: 398 2918 1156 2573
Fragments arranged by size:
2918 2573 1156 398
BmgBI CAC'GTC
Cuts at: 1318 4221 1318
Size: 2903 4142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2134 2203 2941
Size: 416 53 83 399 24 717 69 738
Cuts at: 2941 3275 3854 4776 5734 442
Size: 334 579 922 958 1753
Fragments arranged by size:
1753 958 922 738 717 579 416 399
334 83 69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3528 3528
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3136 3795 4335 5705 1239
Size: 1897 659 540 1370 2579
Fragments arranged by size:
2579 1897 1370 659 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2984 5170 6035 6333 2984
Size: 2186 865 298 3696
Fragments arranged by size:
3696 2186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5708 882
Size: 617 4209 2219
Fragments arranged by size:
4209 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4836 493
Size: 711 3632 2702
Fragments arranged by size:
3632 2702 711
BsaBI GATnn'nnATC
Cuts at: 4040 7039 4040
Size: 2999 4046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2055 4852 5234
Size: 53 83 186 936 495 27 2797 382
Cuts at: 5234 5869 275
Size: 635 1451
Fragments arranged by size:
2797 1451 936 635 495 382 186 83
53 27
BsaWI w'CCGG_w
Cuts at: 5478 5876 6150 6297 6708 5478
Size: 398 274 147 411 5815
Fragments arranged by size:
5815 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3193 3223 1178
Size: 30 165 30 1790 30 5000
Fragments arranged by size:
5000 1790 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2317 1186
Size: 1131 5914
BseYI C'CCAG_C
Cuts at: 3143 3493 6248 6744 3143
Size: 350 2755 496 3444
Fragments arranged by size:
3444 2755 496 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2459 2742 2934 3759 1163
Size: 573 411 312 283 192 825 4449
Fragments arranged by size:
4449 825 573 411 312 283 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 4495 5256 6284 1540
Size: 275 186 102 2392 761 1028 2301
Fragments arranged by size:
2392 2301 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 3509 5106 5191 6262 3509
Size: 1597 85 1071 4292
Fragments arranged by size:
4292 1597 1071 85
BsmI GAATG_Cn'
Cuts at: 3951 4556 4649 3951
Size: 605 93 6347
Fragments arranged by size:
6347 605 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2140 2188 2209 3409 3509 3547
Size: 399 286 64 48 21 1200 100 38
Cuts at: 3547 4364 5106 5191 6262 1391
Size: 817 742 85 1071 2174
Fragments arranged by size:
2174 1200 1071 817 742 399 286 100
85 64 48 38 21
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 4051 4180 4270 4934 6664 4051
Size: 129 90 664 1730 4432
Fragments arranged by size:
4432 1730 664 129 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4400 844
Size: 3556 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 4932 1394
Size: 429 401 2708 3507
Fragments arranged by size:
3507 2708 429 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 2816 3110 3589 5540 5714 65
Size: 1183 1413 155 294 479 1951 174 1396
Fragments arranged by size:
1951 1413 1396 1183 479 294 174 155
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2102 5689 5876 1700
Size: 110 247 45 3587 187 2869
Fragments arranged by size:
3587 2869 247 187 110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 2177 5105 6117 2177
Size: 2928 1012 3105
Fragments arranged by size:
3105 2928 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 2660 794
Size: 1376 490 5179
Fragments arranged by size:
5179 1376 490
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstYI r'GATC_y
Cuts at: 1972 2157 2293 3865 4375 4742 5127 5144 6596
Size: 185 136 1572 510 367 385 17 1452
Cuts at: 6596 6682 6694 7040 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1572 1452 510 385 367 346 185
136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 513
Size: 552 939 93 5461
Fragments arranged by size:
5461 939 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2934 487
Size: 683 56 1708 4598
Fragments arranged by size:
4598 1708 683 56
BtsI GCAGTG_nn'
Cuts at: 3599 3883 4044 4561 5355 5375 3599
Size: 284 161 517 794 20 5269
Fragments arranged by size:
5269 794 517 284 161 20
ClaI AT'CG_AT
Cuts at: 1554 4265 1554
Size: 2711 4334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7010
DraI TTT'AAA
Cuts at: 4349 4349
Size: 7045
DraIII CAC_nnn'GTG
Cuts at: 3821 3821
Size: 7045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3297 4492
Size: 54 1425 50 275 18 270 1197 1195
Cuts at: 4492 5381 7010 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1197 1195 889 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 4492 1537
Size: 275 288 2392 4090
Fragments arranged by size:
4090 2392 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2764 3038 3997 4035 4975 1353
Size: 1411 274 959 38 940 3423
Fragments arranged by size:
3423 1411 959 940 274 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 2243 5632 6006 6152 387
Size: 1064 138 513 141 3389 374 146 1280
Fragments arranged by size:
3389 1280 1064 513 374 146 141 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2658 2982 3136 3795 4335 5108
Size: 393 388 638 324 154 659 540 773
Cuts at: 5108 5705 6492 6760 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 773 659 638 597 540 393
388 324 268 154
EcoNI CCTnn'n_nnAGG
Cuts at: 3699 3699
Size: 7045
EcoO109I rG'GnC_Cy
Cuts at: 2021 3692 2021
Size: 1671 5374
EcoRI G'AATT_C
Cuts at: 4232 4354 4732 4232
Size: 122 378 6545
Fragments arranged by size:
6545 378 122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3092 3092
Size: 7045
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 2058 6192 1678
Size: 380 4134 2531
Fragments arranged by size:
4134 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2472 2504 2525 2557 2672
Size: 32 135 32 900 32 21 32 115
Cuts at: 2672 2704 2888 2920 3193 3225 5686 5718 5760
Size: 32 184 32 273 32 2461 32 42
Cuts at: 5760 5792 1373
Size: 32 2626
Fragments arranged by size:
2626 2461 900 273 184 135 115 42
32 32 32 32 32 32 32 32
32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2480 2676 4389 4635 669
Size: 988 281 542 196 1713 246 3079
Fragments arranged by size:
3079 1713 988 542 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7045
HpaI GTT'AAC
Cuts at: 2676 4635 2676
Size: 1959 5086
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 2799 4644 2799
Size: 1845 5200
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 6159 6343 1305
Size: 993 3861 184 2007
Fragments arranged by size:
3861 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3299 10
Size: 54 1425 343 1467 3756
Fragments arranged by size:
3756 1467 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3393 3711 4294 5003 5362 5521 518
Size: 648 2227 318 583 709 359 159 2042
Fragments arranged by size:
2227 2042 709 648 583 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2726 2829 4461 5138 6286 6531 1606
Size: 493 627 103 1632 677 1148 245 2120
Fragments arranged by size:
2120 1632 1148 677 627 493 245 103
NaeI GCC'GGC
Cuts at: 2059 2104 2059
Size: 45 7000
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5554
Fragments arranged by size:
5554 939 552
NdeI CA'TA_TG
Cuts at: 387 3891 387
Size: 3504 3541
NgoMIV G'CCGG_C
Cuts at: 2057 2102 2057
Size: 45 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NsiI A_TGCA'T
Cuts at: 3349 3349
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 4403 5948 1132
Size: 97 3174 1545 2229
Fragments arranged by size:
3174 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7045
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4108 1108
Size: 3000 4045
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2021
Size: 7045
PshAI GACnn'nnGTC
Cuts at: 1613 2949 1613
Size: 1336 5709
PsiI TTA'TAA
Cuts at: 4615 4615
Size: 7045
PspOMI G'GGCC_C
Cuts at: 2184 2184
Size: 7045
PstI C_TGCA'G
Cuts at: 830 2175 2238 2259 2731 4397 830
Size: 1345 63 21 472 1666 3478
Fragments arranged by size:
3478 1666 1345 472 63 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 2726 2726
Size: 7045
SacII CC_GC'GG
Cuts at: 2100 2100
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 2478 4387 1936
Size: 542 1909 4594
Fragments arranged by size:
4594 1909 542
SanDI GG'GwC_CC
Cuts at: 2021 2021
Size: 7045
SbfI CC_TGCA'GG
Cuts at: 2259 4397 2259
Size: 2138 4907
ScaI AGT'ACT
Cuts at: 5293 7005 5293
Size: 1712 5333
SfcI C'TryA_G
Cuts at: 826 1045 2171 2234 2255 2501 2727 4393 5528
Size: 219 1126 63 21 246 226 1666 1135
Cuts at: 5528 6209 6400 826
Size: 681 191 1471
Fragments arranged by size:
1666 1471 1135 1126 681 246 226 219
191 63 21
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 2057 1700
Size: 357 6688
SmaI CCC'GGG
Cuts at: 1872 2052 2154 4372 1872
Size: 180 102 2218 4545
Fragments arranged by size:
4545 2218 180 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2999 5149 6050 6312 820
Size: 197 926 1056 2150 901 262 1553
Fragments arranged by size:
2150 1553 1056 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 3288 1517
Size: 1771 5274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 4484 513
Size: 552 939 63 2417 3074
Fragments arranged by size:
3074 2417 939 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4302 5072 5089 5242 5427 5872 325
Size: 1255 2722 770 17 153 185 445 1498
Fragments arranged by size:
2722 1498 1255 770 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 5291 7003
Size: 274 80 33 51 1443 165 3149 1712
Cuts at: 7003 96
Size: 138
Fragments arranged by size:
3149 1712 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 7005 7037 2933
Size: 32 4040 32 2941
Fragments arranged by size:
4040 2941 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 2530 2906 4207 4381 2530
Size: 376 1301 174 5194
Fragments arranged by size:
5194 1301 376 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 2050 2152 4370 1870
Size: 180 102 2218 4545
Fragments arranged by size:
4545 2218 180 102
XmnI GAAnn'nnTTC
Cuts at: 4266 5174 4266
Size: 908 6137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
NruI PacI PfoI PmlI PspXI PsrI RsrII SacI
SapI SexAI SfiI SrfI SwaI XcmI