Restriction map for
pFN21AB5794
(Linear) (Six-base) MAPSORT of: KIBB5794.seq Check: 4877 from: 1 to: 2340
KIBB5794.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 474 2340
Size: 474 1866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 653 977 2340
Size: 15 638 324 1363
Fragments arranged by size:
1363 638 324 15
AflIII A'CryG_T
Cuts at: 0 2215 2340
Size: 2215 125
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1829 1861 2340
Size: 1829 32 479
Fragments arranged by size:
1829 479 32
AlwNI CAG_nnn'CTG
Cuts at: 0 631 679 2340
Size: 631 48 1661
Fragments arranged by size:
1661 631 48
ApaI G_GGCC'C
Cuts at: 0 183 2340
Size: 183 2157
ApoI r'AATT_y
Cuts at: 0 276 703 1470 2065 2227 2340
Size: 276 427 767 595 162 113
Fragments arranged by size:
767 595 427 276 162 113
AseI AT'TA_AT
Cuts at: 0 1169 2340
Size: 1169 1171
AvaI C'yCGr_G
Cuts at: 0 45 70 128 147 1878 2340
Size: 45 25 58 19 1731 462
Fragments arranged by size:
1731 462 58 45 25 19
BamHI G'GATC_C
Cuts at: 0 152 2340
Size: 152 2188
BanI G'GyrC_C
Cuts at: 0 49 58 2340
Size: 49 9 2282
Fragments arranged by size:
2282 49 9
BanII G_rGCy'C
Cuts at: 0 71 135 183 204 1404 1542 2340
Size: 71 64 48 21 1200 138 798
Fragments arranged by size:
1200 798 138 71 64 48 21
BbeI G_GCGC'C
Cuts at: 0 53 2340
Size: 53 2287
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 944 1357 2057 2340
Size: 18 926 413 700 283
Fragments arranged by size:
926 700 413 283 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 2230 2340
Size: 2230 110
BfrBI ATG'CAT
Cuts at: 0 1342 2340
Size: 1342 998
Bme1580I G_kGCm'C
Cuts at: 0 183 2340
Size: 183 2157
BmgBI CAC'GTC
Cuts at: 0 2216 2340
Size: 2216 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 129 198 936 1270 1849 2340
Size: 129 69 738 334 579 491
Fragments arranged by size:
738 579 491 334 129 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1523 2340
Size: 1523 817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 1790 2330 2340
Size: 1131 659 540 10
Fragments arranged by size:
1131 659 540 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 2340
Size: 979 1361
BsaBI GATnn'nnATC
Cuts at: 0 2035 2340
Size: 2035 305
BsaHI Gr'CG_yC
Cuts at: 0 23 50 2340
Size: 23 27 2290
Fragments arranged by size:
2290 27 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1188 1218 2340
Size: 1188 30 1122
Fragments arranged by size:
1188 1122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 312 2340
Size: 312 2028
BseYI C'CCAG_C
Cuts at: 0 1138 1488 2340
Size: 1138 350 852
Fragments arranged by size:
1138 852 350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 454 737 929 1754 2340
Size: 142 312 283 192 825 586
Fragments arranged by size:
825 586 312 283 192 142
BsiEI CG_ry'CG
Cuts at: 0 98 2340
Size: 98 2242
BsiHKAI G_wGCw'C
Cuts at: 0 1504 2340
Size: 1504 836
BsmI GAATG_Cn'
Cuts at: 0 1946 2340
Size: 1946 394
Bsp1286I G_dGCh'C
Cuts at: 0 71 135 183 204 1404 1504 1542 2340
Size: 71 64 48 21 1200 100 38 798
Fragments arranged by size:
1200 798 100 71 64 48 38 21
BspHI T'CATG_A
Cuts at: 0 2046 2175 2265 2340
Size: 2046 129 90 75
Fragments arranged by size:
2046 129 90 75
BsrBI CCG'CTC
Cuts at: 0 219 2340
Size: 219 2121
BsrDI GCAATG_nn'
Cuts at: 0 656 811 1105 1584 2340
Size: 656 155 294 479 756
Fragments arranged by size:
756 656 479 294 155
BsrFI r'CCGG_y
Cuts at: 0 52 97 2340
Size: 52 45 2243
Fragments arranged by size:
2243 52 45
BssSI C'ACGA_G
Cuts at: 0 172 2340
Size: 172 2168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 165 655 2340
Size: 165 490 1685
Fragments arranged by size:
1685 490 165
BstYI r'GATC_y
Cuts at: 0 152 288 1860 2340
Size: 152 136 1572 480
Fragments arranged by size:
1572 480 152 136
BtgI C'CryG_G
Cuts at: 0 92 2340
Size: 92 2248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 929 2340
Size: 929 1411
BtsI GCAGTG_nn'
Cuts at: 0 1594 1878 2039 2340
Size: 1594 284 161 301
Fragments arranged by size:
1594 301 284 161
ClaI AT'CG_AT
Cuts at: 0 2260 2340
Size: 2260 80
DraIII CAC_nnn'GTG
Cuts at: 0 1816 2340
Size: 1816 524
EaeI y'GGCC_r
Cuts at: 0 95 1292 2340
Size: 95 1197 1048
Fragments arranged by size:
1197 1048 95
EagI C'GGCC_G
Cuts at: 0 95 2340
Size: 95 2245
EarI CTCTTCn'nnn_
Cuts at: 0 759 1033 1992 2030 2340
Size: 759 274 959 38 310
Fragments arranged by size:
959 759 310 274 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 238 2340
Size: 97 141 2102
Fragments arranged by size:
2102 141 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 653 977 1131 1790 2330 2340
Size: 15 638 324 154 659 540 10
Fragments arranged by size:
659 638 540 324 154 15 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1694 2340
Size: 1694 646
EcoO109I rG'GnC_Cy
Cuts at: 0 16 1687 2340
Size: 16 1671 653
Fragments arranged by size:
1671 653 16
EcoRI G'AATT_C
Cuts at: 0 2227 2340
Size: 2227 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1087 2340
Size: 1087 1253
HaeII r_GCGC'y
Cuts at: 0 53 2340
Size: 53 2287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 520 552 667 699 883 915
Size: 467 32 21 32 115 32 184 32
Cuts at: 915 1188 1220 2340
Size: 273 32 1120
Fragments arranged by size:
1120 467 273 184 115 32 32 32
32 32 21
HincII GTy'rAC
Cuts at: 0 475 671 2340
Size: 475 196 1669
Fragments arranged by size:
1669 475 196
HpaI GTT'AAC
Cuts at: 0 671 2340
Size: 671 1669
KasI G'GCGC_C
Cuts at: 0 49 2340
Size: 49 2291
MfeI C'AATT_G
Cuts at: 0 794 2340
Size: 794 1546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 2340
Size: 293 2047
MscI TGG'CCA
Cuts at: 0 1294 2340
Size: 1294 1046
MslI CAynn'nnrTG
Cuts at: 0 1388 1706 2289 2340
Size: 1388 318 583 51
Fragments arranged by size:
1388 583 318 51
MspA1I CmG'CkG
Cuts at: 0 94 721 824 2340
Size: 94 627 103 1516
Fragments arranged by size:
1516 627 103 94
NaeI GCC'GGC
Cuts at: 0 54 99 2340
Size: 54 45 2241
Fragments arranged by size:
2241 54 45
NarI GG'CG_CC
Cuts at: 0 50 2340
Size: 50 2290
NdeI CA'TA_TG
Cuts at: 0 1886 2340
Size: 1886 454
NgoMIV G'CCGG_C
Cuts at: 0 52 97 2340
Size: 52 45 2243
Fragments arranged by size:
2243 52 45
NsiI A_TGCA'T
Cuts at: 0 1344 2340
Size: 1344 996
PflMI CCAn_nnn'nTGG
Cuts at: 0 2103 2340
Size: 2103 237
PpuMI rG'GwC_Cy
Cuts at: 0 16 2340
Size: 16 2324
PshAI GACnn'nnGTC
Cuts at: 0 944 2340
Size: 944 1396
PspOMI G'GGCC_C
Cuts at: 0 179 2340
Size: 179 2161
PstI C_TGCA'G
Cuts at: 0 170 233 254 726 2340
Size: 170 63 21 472 1614
Fragments arranged by size:
1614 472 170 63 21
PvuII CAG'CTG
Cuts at: 0 721 2340
Size: 721 1619
SacII CC_GC'GG
Cuts at: 0 95 2340
Size: 95 2245
SalI G'TCGA_C
Cuts at: 0 473 2340
Size: 473 1867
SanDI GG'GwC_CC
Cuts at: 0 16 2340
Size: 16 2324
SbfI CC_TGCA'GG
Cuts at: 0 254 2340
Size: 254 2086
SfcI C'TryA_G
Cuts at: 0 166 229 250 496 722 2340
Size: 166 63 21 246 226 1618
Fragments arranged by size:
1618 246 226 166 63 21
SfoI GGC'GCC
Cuts at: 0 51 2340
Size: 51 2289
SgrAI Cr'CCGG_yG
Cuts at: 0 52 2340
Size: 52 2288
SmaI CCC'GGG
Cuts at: 0 47 149 2340
Size: 47 102 2191
Fragments arranged by size:
2191 102 47
SmlI C'TyrA_G
Cuts at: 0 994 2340
Size: 994 1346
StuI AGG'CCT
Cuts at: 0 1283 2340
Size: 1283 1057
StyI C'CwwG_G
Cuts at: 0 62 2340
Size: 62 2278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2297 2340
Size: 2297 43
TatI w'GTAC_w
Cuts at: 0 137 2340
Size: 137 2203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 928 960 2340
Size: 928 32 1380
Fragments arranged by size:
1380 928 32
XbaI T'CTAG_A
Cuts at: 0 525 901 2202 2340
Size: 525 376 1301 138
Fragments arranged by size:
1301 525 376 138
XmaI C'CCGG_G
Cuts at: 0 45 147 2340
Size: 45 102 2193
Fragments arranged by size:
2193 102 45
XmnI GAAnn'nnTTC
Cuts at: 0 2261 2340
Size: 2261 79
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstYI BtgI
BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI PflMI PpuMI PshAI PspOMI
PstI PvuII SacII SalI SanDI SbfI SfcI SfoI
SgrAI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AsiSI AvrII BaeI BbvCI
BcgI BclI BglI BglII BlpI BmtI Bpu10I BsaI
BsaAI BsaWI BsiWI BsmBI BspEI BspMI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I CspCI DraI DrdI
EcoICRI EcoRV FseI FspI FspAI HindIII KpnI MluI
NcoI NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SapI ScaI SexAI SfiI SgfI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XcmI XhoI
ZraI