##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
TseI        Tsp45I      TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AclI           76  AACGTT               AA^CGTT
AcsI          244  GAATTT               [GA]^AATT[CT]
AfaI           60  GTAC                 GT^AC
AlwI          171  GGATC                GGATC(4/5)
AlwNI          13  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          244  GAATTT               [GA]^AATT[CT]
AseI          106  ATTAAT               AT^TAAT
AsnI          106  ATTAAT               AT^TAAT
BfaI          250  CTAG                 C^TAG
BglII         138  AGATCT               A^GATCT
BsaI           64  GGTCTC               GGTCTC(1/5)
BsmAI          65  GTCTC                GTCTC(1/5)
BspMI          33  GCAGGT               ACCTGC(4/8)
BsrI          144  CCAGT                ACTGG(1/-1)
BssKI         168  CCTGG                ^CC[ACGT]GG
BssSI         158  CTCGTG               CACGAG(-5/-1)
BstNI         168  CCTGG                CC^[AT]GG
BstYI         138  AGATCT               [GA]^GATC[CT]
Cac8I         213  GCTTGC               GC[ACGT]^[ACGT]GC
DdeI           56  CTGAG                C^T[ACGT]AG
DraI          264  TTTAAA               TTT^AAA
EcoRII        168  CCTGG                ^CC[AT]GG
MaeI          250  CTAG                 C^TAG
MaeII          77  ACGT                 A^CGT
MflI          138  AGATCT               [GA]^GATC[CT]
MvaI          168  CCTGG                CC^[AT]GG
MwoI          208  GCTGTGCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV           3  GGAGCC               GG[ACGT]^[ACGT]CC
PshBI         106  ATTAAT               AT^TAAT
Psp1406I       76  AACGTT               AA^CGTT
RsaI           60  GTAC                 GT^AC
ScrFI         168  CCTGG                CC^[ACGT]GG
TaqI          291  TCGA                 T^CGA
Tsp509I       245  AATT                 ^AATT
TthHB8I       291  TCGA                 T^CGA
XhoII         138  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BpmI          142  CTCCAG               CTGGAG(16/14)
               19  CTGGAG              
BsmFI          24  GGGAC                GGGAC(10/14)
              201  GGGAC               
MboII         102  GAAGA                GAAGA(8/7)
              286  GAAGA               
MnlI           49  CCTC                 CCTC(7/6)
               22  GAGG                
MseI          107  TTAA                 T^TAA
              265  TTAA                
SfaNI          15  GATGC                GCATC(5/9)
              253  GCATC               
Tru9I         107  TTAA                 T^TAA
              265  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

DpnII       MboI        NdeII       Sau3AI