################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AocI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsiWI BsmBI BspDI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eco81I EcoRV FbaI FspI HpaI KasI KpnI KspI MluI MseI NarI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tru9I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2295 GTCTAC GT^[AC][GT]AC AccII 3736 CGCG CG^CG AflIII 1454 ACGTGT A^C[GA][CT]GT AhdI 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1524 GTGCAC G^TGCAC Aor51HI 3247 AGCGCT AGC^GCT ApaLI 1524 GTGCAC G^TGCAC AspEI 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 707 GGATAC GTATCC(6/5) BsaBI 3183 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2407 GAGACC GGTCTC(1/5) BsiEI 2460 CGGCCG CG[GA][CT]^CG BstUI 3736 CGCG CG^CG DraIII 3945 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2460 CGGCCG C^GGCCG Eam1105I 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2460 CGGCCG C^GGCCG Eco47III 3247 AGCGCT AGC^GCT Eco52I 2460 CGGCCG C^GGCCG EcoRI 861 GAATTC G^AATTC FseI 1723 GGCCGGCC GGCCGG^CC HaeII 3247 AGCGCT [GA]GCGC^[CT] HincII 2953 GTTGAC GT[CT]^[GA]AC HindII 2953 GTTGAC GT[CT]^[GA]AC MamI 3183 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2093 CAATTG C^AATTG MunI 2093 CAATTG C^AATTG MvnI 3736 CGCG CG^CG NaeI 1724 GCCGGC GCC^GGC NcoI 404 CCATGG C^CATGG NgoMIV 1724 GCCGGC G^CCGGC PaeR7I 3845 CTCGAG C^TCGAG PflFI 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2238 GACTC GAGTC(4/5) SapI 1677 GCTCTTC GCTCTTC(1/4) SfiI 1098 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1835 CCCGGG CCC^GGG SphI 2477 GCATGC GCATG^C Tth111I 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3845 CTCGAG C^TCGAG XmaCI 1835 CCCGGG C^CCGGG XmaI 1835 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 383 TCCGGA T^CCGGA 479 TCCGGA AcsI 861 GAATTC [GA]^AATT[CT] 1943 GAATTT AcyI 760 GACGCC G[GA]^CG[CT]C 1035 GGCGTC ApaI 1838 GGGCCC GGGCC^C 2749 GGGCCC ApoI 861 GAATTC [GA]^AATT[CT] 1943 GAATTT Asp700I 420 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3444 GAACCCCTTC BamHI 612 GGATCC G^GATCC 848 GGATCC BanI 1132 GGCACC G^G[CT][GA]CC 1689 GGTGCC BbvCI 1536 CCTCAGC CCTCAGC(-5/-2) 648 GCTGAGG BglI 214 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1099 GCCAGTGGGGC BsaHI 760 GACGCC G[GA]^CG[CT]C 1035 GGCGTC BseAI 383 TCCGGA T^CCGGA 479 TCCGGA BspEI 383 TCCGGA T^CCGGA 479 TCCGGA BspHI 362 TCATGA T^CATGA 2552 TCATGA BsrFI 1724 GCCGGC [GA]^CCGG[CT] 2458 ACCGGC BstAPI 294 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3920 GCAATGCCTGC BstEII 1299 GGTAACC G^GT[ACGT]ACC 1824 GGTCACC BstPI 1299 GGTAACC G^GT[ACGT]ACC 1824 GGTCACC BstXI 2429 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3603 CCAGGGCCTTGG CfoI 1002 GCGC GCG^C 3248 GCGC Cfr10I 1724 GCCGGC [GA]^CCGG[CT] 2458 ACCGGC DrdI 556 GACCACTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1116 GACACTTGTGTC EcoO65I 1299 GGTAACC G^GT[ACGT]ACC 1824 GGTCACC EcoT22I 2475 ATGCAT ATGCA^T 3764 ATGCAT FauI 143 GCGGG CCCGC(4/6) 338 GCGGG HgaI 760 GACGC GACGC(5/10) 1036 GCGTC HhaI 1002 GCGC GCG^C 3248 GCGC Hin1I 760 GACGCC G[GA]^CG[CT]C 1035 GGCGTC HinP1I 1002 GCGC G^CGC 3248 GCGC MroI 383 TCCGGA T^CCGGA 479 TCCGGA NsiI 2475 ATGCAT ATGCA^T 3764 ATGCAT PflMI 1424 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2897 CCAGATGCTGG RcaI 362 TCATGA T^CATGA 2552 TCATGA Van91I 1424 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2897 CCAGATGCTGG XcmI 209 CCAATGCCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 764 CCAAGCTGGTCGTGG XmnI 420 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3444 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII