(Circular) (Six-base) MAPSORT of: pFN21AE2474.seq Check: 4242 from: 1 to: 8704 pFN21AE2474 8704 bp With 182 enzymes: SgfI * June 25, 2013 12:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6514 278 Size: 53 83 186 936 4978 2468 Fragments arranged by size: 4978 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4301 6047 1937 Size: 2364 1746 4594 Fragments arranged by size: 4594 2364 1746 Acc65I G'GTAC_C Cuts at: 6025 6025 Size: 8704 AclI AA'CG_TT Cuts at: 1563 6831 7204 1563 Size: 5268 373 3063 Fragments arranged by size: 5268 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4163 4582 5449 5508 6767 8151 8419 Size: 1928 603 419 867 59 1259 1384 268 Cuts at: 8419 1632 Size: 1917 Fragments arranged by size: 1928 1917 1384 1259 867 603 419 268 59 AfeI AGC'GCT Cuts at: 5254 5254 Size: 8704 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8507 AflIII A'CryG_T Cuts at: 1203 3459 7603 8580 1203 Size: 2256 4144 977 1327 Fragments arranged by size: 4144 2256 1327 977 AgeI A'CCGG_T Cuts at: 7535 7535 Size: 8704 AhdI GACnn_n'nnGTC Cuts at: 5273 7433 5273 Size: 2160 6544 AleI CACnn'nnGTG Cuts at: 1166 4427 4439 5670 1166 Size: 3261 12 1231 4200 Fragments arranged by size: 4200 3261 1231 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2180 2268 1184 Size: 996 88 7620 Fragments arranged by size: 7620 996 88 AlwNI CAG_nnn'CTG Cuts at: 3358 3583 4908 6447 8019 3358 Size: 225 1325 1539 1572 4043 Fragments arranged by size: 4043 1572 1539 1325 225 ApaI G_GGCC'C Cuts at: 3847 4758 3847 Size: 911 7793 ApaLI G'TGCA_C Cuts at: 3529 6761 7917 3529 Size: 3232 1156 4316 Fragments arranged by size: 4316 3232 1156 ApoI r'AATT_y Cuts at: 1493 2866 3948 6013 6239 6391 8500 1493 Size: 1373 1082 2065 226 152 2109 1697 Fragments arranged by size: 2109 2065 1697 1373 1082 226 152 AseI AT'TA_AT Cuts at: 160 1032 7258 160 Size: 872 6226 1606 Fragments arranged by size: 6226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8704 AvaI C'yCGr_G Cuts at: 1870 1943 2399 3840 5651 5850 6029 1870 Size: 73 456 1441 1811 199 179 4545 Fragments arranged by size: 4545 1811 1441 456 199 179 73 BamHI G'GATC_C Cuts at: 2617 2853 6034 6401 2617 Size: 236 3181 367 4920 Fragments arranged by size: 4920 3181 367 236 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3137 3694 6025 7480 618 Size: 325 217 627 1350 557 2331 1455 1842 Fragments arranged by size: 2331 1842 1455 1350 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 2406 2634 3203 3847 4293 4758 5065 5167 Size: 1015 228 569 644 446 465 307 102 Cuts at: 5167 5857 6023 1391 Size: 690 166 4072 Fragments arranged by size: 4072 1015 690 644 569 465 446 307 228 166 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3609 4325 4568 5355 928 Size: 971 1710 716 243 787 4277 Fragments arranged by size: 4277 1710 971 787 716 243 BbvCI CC'TCA_GC Cuts at: 2654 3542 2654 Size: 888 7816 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6895 6929 1903 Size: 34 4958 34 3678 Fragments arranged by size: 4958 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2706 6596 7806 2706 Size: 3890 1210 3604 Fragments arranged by size: 3890 3604 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8704 BfrBI ATG'CAT Cuts at: 4482 5771 4482 Size: 1289 7415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2225 3110 7315 Size: 107 122 71 631 605 553 885 4205 Cuts at: 7315 136 Size: 1525 Fragments arranged by size: 4205 1525 885 631 605 553 122 107 71 BglII A'GATC_T Cuts at: 8699 8699 Size: 8704 BlpI GC'TnA_GC Cuts at: 2754 2955 3197 5856 6121 2754 Size: 201 242 2659 265 5337 Fragments arranged by size: 5337 2659 265 242 201 Bme1580I G_kGCm'C Cuts at: 1790 3533 3699 3847 4697 4758 5223 6765 7921 Size: 1743 166 148 850 61 465 1542 1156 Cuts at: 7921 1790 Size: 2573 Fragments arranged by size: 2573 1743 1542 1156 850 465 166 148 61 BmgBI CAC'GTC Cuts at: 1318 2709 3460 1318 Size: 1391 751 6562 Fragments arranged by size: 6562 1391 751 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2332 4575 4707 Size: 416 53 83 399 24 915 2243 132 Cuts at: 4707 5328 5362 5406 6435 7393 442 Size: 621 34 44 1029 958 1753 Fragments arranged by size: 2243 1753 1029 958 915 621 416 399 132 83 53 44 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8704 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3274 5458 3274 Size: 2184 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 3017 4259 5542 7364 1239 Size: 1220 558 1242 1283 1822 2579 Fragments arranged by size: 2579 1822 1283 1242 1220 558 Bpu10I CC'TnA_GC Cuts at: 2654 2859 2918 3542 4137 2654 Size: 205 59 624 595 7221 Fragments arranged by size: 7221 624 595 205 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 2732 3389 4604 4904 6829 7694 7992 2714 Size: 18 657 1215 300 1925 865 298 3426 Fragments arranged by size: 3426 1925 1215 865 657 300 298 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 7367 882 Size: 617 2907 2961 2219 Fragments arranged by size: 2961 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6495 493 Size: 711 5291 2702 Fragments arranged by size: 5291 2702 711 BsaBI GATnn'nnATC Cuts at: 5192 8698 5192 Size: 3506 5198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2766 3041 6511 6893 Size: 53 83 186 936 1233 275 3470 382 Cuts at: 6893 7528 275 Size: 635 1451 Fragments arranged by size: 3470 1451 1233 936 635 382 275 186 83 53 BsaWI w'CCGG_w Cuts at: 2388 2484 3525 4362 7137 7535 7809 7956 8367 Size: 96 1041 837 2775 398 274 147 411 Cuts at: 8367 2388 Size: 2725 Fragments arranged by size: 2775 2725 1041 837 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2457 2487 2879 2909 3236 Size: 30 165 30 1054 30 392 30 327 Cuts at: 3236 3266 4709 4739 1178 Size: 30 1443 30 5143 Fragments arranged by size: 5143 1443 1054 392 327 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 2532 2615 2661 3221 3458 3494 3728 Size: 1243 103 83 46 560 237 36 234 Cuts at: 3728 4407 4838 5648 1186 Size: 679 431 810 4242 Fragments arranged by size: 4242 1243 810 679 560 431 237 234 103 83 46 36 BseYI C'CCAG_C Cuts at: 2845 3017 3583 3667 4908 7907 8403 2845 Size: 172 566 84 1241 2999 496 3146 Fragments arranged by size: 3146 2999 1241 566 496 172 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2335 2437 3486 4234 5648 5865 Size: 573 591 8 102 1049 748 1414 217 Cuts at: 5865 1163 Size: 4002 Fragments arranged by size: 4002 1414 1049 748 591 573 217 102 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4468 6154 6915 7943 1540 Size: 275 186 2467 1686 761 1028 2301 Fragments arranged by size: 2467 2301 1686 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3533 4230 4293 4424 5167 5857 6765 6850 7921 Size: 697 63 131 743 690 908 85 1071 Cuts at: 7921 3533 Size: 4316 Fragments arranged by size: 4316 1071 908 743 697 690 131 85 63 BsmI GAATG_Cn' Cuts at: 2431 3263 4922 6215 6308 2431 Size: 832 1659 1293 93 4827 Fragments arranged by size: 4827 1659 1293 832 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2406 2634 3203 3533 3699 3847 4230 Size: 399 616 228 569 330 166 148 383 Cuts at: 4230 4293 4424 4697 4758 5065 5167 5223 5857 Size: 63 131 273 61 307 102 56 634 Cuts at: 5857 6023 6765 6850 7921 1391 Size: 166 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 616 569 399 383 330 307 273 228 166 166 148 131 102 85 63 61 56 BspEI T'CCGG_A Cuts at: 2388 2484 8367 2388 Size: 96 5883 2725 Fragments arranged by size: 5883 2725 96 BspHI T'CATG_A Cuts at: 2367 4557 6593 8323 2367 Size: 2190 2036 1730 2748 Fragments arranged by size: 2748 2190 2036 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6059 844 Size: 5215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6591 1394 Size: 429 4768 3507 Fragments arranged by size: 4768 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 4254 4609 4770 5932 7199 7373 Size: 1183 2046 960 355 161 1162 1267 174 Cuts at: 7373 65 Size: 1396 Fragments arranged by size: 2046 1396 1267 1183 1162 960 355 174 161 BsrFI r'CCGG_y Cuts at: 1700 1810 3729 4463 7348 7535 1700 Size: 110 1919 734 2885 187 2869 Fragments arranged by size: 2885 2869 1919 734 187 110 BsrGI T'GTAC_A Cuts at: 96 2376 4845 5076 96 Size: 2280 2469 231 3724 Fragments arranged by size: 3724 2469 2280 231 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8704 BssSI C'ACGA_G Cuts at: 6764 7776 6764 Size: 1012 7692 BstAPI GCAn_nnn'nTGC Cuts at: 794 2305 5931 794 Size: 1511 3626 3567 Fragments arranged by size: 3626 3567 1511 BstBI TT'CG_AA Cuts at: 7508 7508 Size: 8704 BstEII G'GTnAC_C Cuts at: 1184 3304 3829 1184 Size: 2120 525 6059 Fragments arranged by size: 6059 2120 525 BstXI CCAn_nnnn'nTGG Cuts at: 4441 5615 4441 Size: 1174 7530 BstYI r'GATC_y Cuts at: 1972 2065 2617 2853 3228 4621 5691 5919 6034 Size: 93 552 236 375 1393 1070 228 115 Cuts at: 6034 6401 6786 6803 8255 8341 8353 8699 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1393 1070 552 385 375 367 346 236 228 115 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2329 2409 4434 5104 5235 5671 513 Size: 552 1264 80 2025 670 131 436 3546 Fragments arranged by size: 3546 2025 1264 670 552 436 131 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4492 487 Size: 683 56 3266 4699 Fragments arranged by size: 4699 3266 683 56 BtsI GCAGTG_nn' Cuts at: 2262 2381 3063 4246 4446 4806 6220 7014 7034 Size: 119 682 1183 200 360 1414 794 20 Cuts at: 7034 2262 Size: 3932 Fragments arranged by size: 3932 1414 1183 794 682 360 200 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5002 5037 529 Size: 35 4438 35 4196 Fragments arranged by size: 4438 4196 35 35 DraI TTT'AAA Cuts at: 6008 6008 Size: 8704 DraIII CAC_nnn'GTG Cuts at: 5955 5955 Size: 8704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2567 3127 7711 809 Size: 507 1251 560 4584 1802 Fragments arranged by size: 4584 1802 1251 560 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3102 3381 4219 Size: 54 1425 50 275 18 1272 279 838 Cuts at: 4219 4465 4536 5154 6151 7040 8669 8 Size: 246 71 618 997 889 1629 43 Fragments arranged by size: 1629 1425 1272 997 889 838 618 279 275 246 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4465 6151 1537 Size: 275 2653 1686 4090 Fragments arranged by size: 4090 2653 1686 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3689 4376 4943 5627 5804 5891 6634 1353 Size: 2336 687 567 684 177 87 743 3423 Fragments arranged by size: 3423 2336 743 687 684 567 177 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7291 7665 7811 387 Size: 1064 138 5702 374 146 1280 Fragments arranged by size: 5702 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4291 5165 5855 4291 Size: 874 690 7140 Fragments arranged by size: 7140 874 690 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 3017 3560 4163 4259 4582 5449 Size: 393 827 558 543 603 96 323 867 Cuts at: 5449 5508 5542 6767 7364 8151 8419 1239 Size: 59 34 1225 597 787 268 1524 Fragments arranged by size: 1524 1225 867 827 787 603 597 558 543 393 323 268 96 59 34 EcoNI CCTnn'n_nnAGG Cuts at: 2494 3238 4166 2494 Size: 744 928 7032 Fragments arranged by size: 7032 928 744 EcoO109I rG'GnC_Cy Cuts at: 2992 4159 4754 4755 4858 5611 2992 Size: 1167 595 1 103 753 6085 Fragments arranged by size: 6085 1167 753 595 103 1 EcoRI G'AATT_C Cuts at: 2866 6013 6391 2866 Size: 3147 378 5179 Fragments arranged by size: 5179 3147 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4570 5902 4570 Size: 1332 7372 FseI GG_CCGG'CC Cuts at: 3733 3733 Size: 8704 FspI TGC'GCA Cuts at: 7210 7210 Size: 8704 HaeII r_GCGC'y Cuts at: 1678 5256 7851 1678 Size: 3578 2595 2531 Fragments arranged by size: 3578 2595 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 2457 2489 2879 Size: 32 135 32 712 32 141 32 390 Cuts at: 2879 2911 3042 3074 5259 5291 5540 5572 7345 Size: 32 131 32 2185 32 249 32 1773 Cuts at: 7345 7377 7419 7451 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2185 1773 712 390 249 141 135 131 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4960 6048 6294 669 Size: 988 281 3022 1088 246 3079 Fragments arranged by size: 3079 3022 1088 988 281 246 HindIII A'AGCT_T Cuts at: 748 2957 4880 5029 748 Size: 2209 1923 149 4423 Fragments arranged by size: 4423 2209 1923 149 HpaI GTT'AAC Cuts at: 6294 6294 Size: 8704 KpnI G_GTAC'C Cuts at: 6029 6029 Size: 8704 MfeI C'AATT_G Cuts at: 4098 6303 4098 Size: 2205 6499 MluI A'CGCG_T Cuts at: 8580 8580 Size: 8704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2645 3028 3105 4752 4964 5764 7818 8002 Size: 1340 383 77 1647 212 800 2054 184 Cuts at: 8002 1305 Size: 2007 Fragments arranged by size: 2054 2007 1647 1340 800 383 212 184 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 3104 3383 4221 4538 5156 Size: 54 1425 343 1272 279 838 317 618 Cuts at: 5156 10 Size: 3558 Fragments arranged by size: 3558 1425 1272 838 618 343 317 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2366 2372 2804 4427 4439 5670 Size: 648 996 204 6 432 1623 12 1231 Cuts at: 5670 6662 7021 7180 518 Size: 992 359 159 2042 Fragments arranged by size: 2042 1623 1231 996 992 648 432 359 204 159 12 6 MspA1I CmG'CkG Cuts at: 1606 2451 2683 3004 3752 3917 5363 6120 6797 Size: 845 232 321 748 165 1446 757 677 Cuts at: 6797 7945 8190 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1446 1148 845 757 748 677 321 245 232 165 NaeI GCC'GGC Cuts at: 3731 3731 Size: 8704 NcoI C'CATG_G Cuts at: 513 1065 2409 513 Size: 552 1344 6808 Fragments arranged by size: 6808 1344 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8704 NgoMIV G'CCGG_C Cuts at: 3729 3729 Size: 8704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8704 NotI GC'GGCC_GC Cuts at: 6151 6151 Size: 8704 NsiI A_TGCA'T Cuts at: 4484 5773 4484 Size: 1289 7415 NspI r_CATG'y Cuts at: 1132 1229 3760 4486 4507 6062 7607 1132 Size: 97 2531 726 21 1555 1545 2229 Fragments arranged by size: 2531 2229 1555 1545 726 97 21 PasI CC'CwG_GG Cuts at: 1348 4759 1348 Size: 3411 5293 PciI A'CATG_T Cuts at: 7603 7603 Size: 8704 PflMI CCAn_nnn'nTGG Cuts at: 1108 3435 4908 1108 Size: 2327 1473 4904 Fragments arranged by size: 4904 2327 1473 PfoI T'CCnGG_A Cuts at: 2994 3278 2994 Size: 284 8420 PmeI GTTT'AAAC Cuts at: 6008 6008 Size: 8704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2795 2827 6773 6805 8286 8318 2795 Size: 32 3946 32 1481 32 3181 Fragments arranged by size: 3946 3181 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2992 4159 4858 2992 Size: 1167 699 6838 Fragments arranged by size: 6838 1167 699 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8704 PsiI TTA'TAA Cuts at: 6274 6274 Size: 8704 PspOMI G'GGCC_C Cuts at: 3843 4754 3843 Size: 911 7793 PspXI vC'TCGA_Gb Cuts at: 5850 5850 Size: 8704 PstI C_TGCA'G Cuts at: 830 2190 3456 4822 6056 830 Size: 1360 1266 1366 1234 3478 Fragments arranged by size: 3478 1366 1360 1266 1234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8704 PvuII CAG'CTG Cuts at: 2683 3004 5363 2683 Size: 321 2359 6024 Fragments arranged by size: 6024 2359 321 SacI G_AGCT'C Cuts at: 4293 5167 5857 4293 Size: 874 690 7140 Fragments arranged by size: 7140 874 690 SalI G'TCGA_C Cuts at: 1936 6046 1936 Size: 4110 4594 SapI GCTCTTCn'nnn_ Cuts at: 3689 3689 Size: 8704 SbfI CC_TGCA'GG Cuts at: 6056 6056 Size: 8704 ScaI AGT'ACT Cuts at: 6952 8664 6952 Size: 1712 6992 SfcI C'TryA_G Cuts at: 826 1045 2186 2461 3181 3235 3452 4818 5425 Size: 219 1141 275 720 54 217 1366 607 Cuts at: 5425 6052 7187 7868 8059 826 Size: 627 1135 681 191 1471 Fragments arranged by size: 1471 1366 1141 1135 720 681 627 607 275 219 217 191 54 SfiI GGCCn_nnn'nGGCC Cuts at: 3110 3110 Size: 8704 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8704 SmaI CCC'GGG Cuts at: 1872 3842 6031 1872 Size: 1970 2189 4545 Fragments arranged by size: 4545 2189 1970 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 2747 3404 4583 4883 5850 Size: 197 926 786 18 657 1179 300 967 Cuts at: 5850 6808 7709 7971 820 Size: 958 901 262 1553 Fragments arranged by size: 1553 1179 967 958 926 901 786 657 300 262 197 18 SnaBI TAC'GTA Cuts at: 493 6495 493 Size: 6002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8704 SphI G_CATG'C Cuts at: 1132 4486 6062 1132 Size: 3354 1576 3774 Fragments arranged by size: 3774 3354 1576 SspI AAT'ATT Cuts at: 5 52 6628 5 Size: 47 6576 2081 Fragments arranged by size: 6576 2081 47 StuI AGG'CCT Cuts at: 1517 2720 3275 3764 5130 1517 Size: 1203 555 489 1366 5091 Fragments arranged by size: 5091 1366 1203 555 489 StyI C'CwwG_G Cuts at: 513 1065 2409 2434 3300 3699 4054 5125 5614 Size: 552 1344 25 866 399 355 1071 489 Cuts at: 5614 6143 513 Size: 529 3074 Fragments arranged by size: 3074 1344 1071 866 552 529 489 399 355 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4631 5428 6731 6748 6901 7086 7531 Size: 1255 3051 797 1303 17 153 185 445 Cuts at: 7531 325 Size: 1498 Fragments arranged by size: 3051 1498 1303 1255 797 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 3780 4845 Size: 274 80 33 51 1443 399 1404 1065 Cuts at: 4845 5076 6950 8662 96 Size: 231 1874 1712 138 Fragments arranged by size: 1874 1712 1443 1404 1065 399 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3512 3544 3593 3625 8664 8696 3512 Size: 32 49 32 5039 32 3520 Fragments arranged by size: 5039 3520 49 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5354 1316 Size: 4038 4666 XbaI T'CTAG_A Cuts at: 6040 6040 Size: 8704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2776 2221 Size: 555 8149 XhoI C'TCGA_G Cuts at: 1943 5850 1943 Size: 3907 4797 XmaI C'CCGG_G Cuts at: 1870 3840 6029 1870 Size: 1970 2189 4545 Fragments arranged by size: 4545 2189 1970 XmnI GAAnn'nnTTC Cuts at: 2429 5453 6833 2429 Size: 3024 1380 4300 Fragments arranged by size: 4300 3024 1380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6512 276 Size: 53 83 186 936 4978 2468 Fragments arranged by size: 4978 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI