(Linear) (Six-base) MAPSORT of: KIEE2474.seq Check: 1287 from: 1 to: 3999 KIEE2474.orf With 182 enzymes: SgfI * June 25, 2013 10:30 .. AccI GT'mk_AC Cuts at: 0 2296 3999 Size: 2296 1703 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1555 2158 2577 3444 3503 3999 Size: 1555 603 419 867 59 496 Fragments arranged by size: 1555 867 603 496 419 59 AfeI AGC'GCT Cuts at: 0 3249 3999 Size: 3249 750 AflIII A'CryG_T Cuts at: 0 1454 3999 Size: 1454 2545 AhdI GACnn_n'nnGTC Cuts at: 0 3268 3999 Size: 3268 731 AleI CACnn'nnGTG Cuts at: 0 2422 2434 3665 3999 Size: 2422 12 1231 334 Fragments arranged by size: 2422 1231 334 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 175 263 3999 Size: 175 88 3736 Fragments arranged by size: 3736 175 88 AlwNI CAG_nnn'CTG Cuts at: 0 1353 1578 2903 3999 Size: 1353 225 1325 1096 Fragments arranged by size: 1353 1325 1096 225 ApaI G_GGCC'C Cuts at: 0 1842 2753 3999 Size: 1842 911 1246 Fragments arranged by size: 1842 1246 911 ApaLI G'TGCA_C Cuts at: 0 1524 3999 Size: 1524 2475 ApoI r'AATT_y Cuts at: 0 861 1943 3999 Size: 861 1082 2056 Fragments arranged by size: 2056 1082 861 AvaI C'yCGr_G Cuts at: 0 394 1835 3646 3845 3999 Size: 394 1441 1811 199 154 Fragments arranged by size: 1811 1441 394 199 154 BamHI G'GATC_C Cuts at: 0 612 848 3999 Size: 612 236 3151 Fragments arranged by size: 3151 612 236 BanI G'GyrC_C Cuts at: 0 1132 1689 3999 Size: 1132 557 2310 Fragments arranged by size: 2310 1132 557 BanII G_rGCy'C Cuts at: 0 401 629 1198 1842 2288 2753 3060 3162 Size: 401 228 569 644 446 465 307 102 Cuts at: 3162 3852 3999 Size: 690 147 Fragments arranged by size: 690 644 569 465 446 401 307 228 147 102 BbsI GAAGACnn'nnnn_ Cuts at: 0 1604 2320 2563 3350 3999 Size: 1604 716 243 787 649 Fragments arranged by size: 1604 787 716 649 243 BbvCI CC'TCA_GC Cuts at: 0 649 1537 3999 Size: 649 888 2462 Fragments arranged by size: 2462 888 649 BciVI GTATCCnnnnn_n' Cuts at: 0 701 3999 Size: 701 3298 BfrBI ATG'CAT Cuts at: 0 2477 3766 3999 Size: 2477 1289 233 Fragments arranged by size: 2477 1289 233 BglI GCCn_nnn'nGGC Cuts at: 0 220 1105 3999 Size: 220 885 2894 Fragments arranged by size: 2894 885 220 BlpI GC'TnA_GC Cuts at: 0 749 950 1192 3851 3999 Size: 749 201 242 2659 148 Fragments arranged by size: 2659 749 242 201 148 Bme1580I G_kGCm'C Cuts at: 0 1528 1694 1842 2692 2753 3218 3999 Size: 1528 166 148 850 61 465 781 Fragments arranged by size: 1528 850 781 465 166 148 61 BmgBI CAC'GTC Cuts at: 0 704 1455 3999 Size: 704 751 2544 Fragments arranged by size: 2544 751 704 BmrI ACTGGGnnnn_n' Cuts at: 0 327 2570 2702 3323 3357 3401 3999 Size: 327 2243 132 621 34 44 598 Fragments arranged by size: 2243 621 598 327 132 44 34 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1269 3453 3999 Size: 1269 2184 546 Fragments arranged by size: 2184 1269 546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 1012 2254 3537 3999 Size: 454 558 1242 1283 462 Fragments arranged by size: 1283 1242 558 462 454 Bpu10I CC'TnA_GC Cuts at: 0 649 854 913 1537 2132 3999 Size: 649 205 59 624 595 1867 Fragments arranged by size: 1867 649 624 595 205 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 727 1384 2599 2899 3999 Size: 709 18 657 1215 300 1100 Fragments arranged by size: 1215 1100 709 657 300 18 BsaI GGTCTCn'nnnn_ Cuts at: 0 2401 3999 Size: 2401 1598 BsaBI GATnn'nnATC Cuts at: 0 3187 3999 Size: 3187 812 BsaHI Gr'CG_yC Cuts at: 0 761 1036 3999 Size: 761 275 2963 Fragments arranged by size: 2963 761 275 BsaWI w'CCGG_w Cuts at: 0 383 479 1520 2357 3999 Size: 383 96 1041 837 1642 Fragments arranged by size: 1642 1041 837 383 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 452 482 874 904 1231 1261 2704 2734 Size: 452 30 392 30 327 30 1443 30 Cuts at: 2734 3999 Size: 1265 Fragments arranged by size: 1443 1265 452 392 327 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 424 527 610 656 1216 1453 1489 1723 Size: 424 103 83 46 560 237 36 234 Cuts at: 1723 2402 2833 3643 3999 Size: 679 431 810 356 Fragments arranged by size: 810 679 560 431 424 356 237 234 103 83 46 36 BseYI C'CCAG_C Cuts at: 0 840 1012 1578 1662 2903 3999 Size: 840 172 566 84 1241 1096 Fragments arranged by size: 1241 1096 840 566 172 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 330 432 1481 2229 3643 3860 3999 Size: 322 8 102 1049 748 1414 217 139 Fragments arranged by size: 1414 1049 748 322 217 139 102 8 BsiEI CG_ry'CG Cuts at: 0 2463 3999 Size: 2463 1536 BsiHKAI G_wGCw'C Cuts at: 0 1528 2225 2288 2419 3162 3852 3999 Size: 1528 697 63 131 743 690 147 Fragments arranged by size: 1528 743 697 690 147 131 63 BsmI GAATG_Cn' Cuts at: 0 426 1258 2917 3999 Size: 426 832 1659 1082 Fragments arranged by size: 1659 1082 832 426 Bsp1286I G_dGCh'C Cuts at: 0 401 629 1198 1528 1694 1842 2225 2288 Size: 401 228 569 330 166 148 383 63 Cuts at: 2288 2419 2692 2753 3060 3162 3218 3852 3999 Size: 131 273 61 307 102 56 634 147 Fragments arranged by size: 634 569 401 383 330 307 273 228 166 148 147 131 102 63 61 56 BspEI T'CCGG_A Cuts at: 0 383 479 3999 Size: 383 96 3520 Fragments arranged by size: 3520 383 96 BspHI T'CATG_A Cuts at: 0 362 2552 3999 Size: 362 2190 1447 Fragments arranged by size: 2190 1447 362 BsrDI GCAATG_nn' Cuts at: 0 1289 2249 2604 2765 3927 3999 Size: 1289 960 355 161 1162 72 Fragments arranged by size: 1289 1162 960 355 161 72 BsrFI r'CCGG_y Cuts at: 0 1724 2458 3999 Size: 1724 734 1541 Fragments arranged by size: 1724 1541 734 BsrGI T'GTAC_A Cuts at: 0 371 2840 3071 3999 Size: 371 2469 231 928 Fragments arranged by size: 2469 928 371 231 BstAPI GCAn_nnn'nTGC Cuts at: 0 300 3926 3999 Size: 300 3626 73 Fragments arranged by size: 3626 300 73 BstEII G'GTnAC_C Cuts at: 0 1299 1824 3999 Size: 1299 525 2175 Fragments arranged by size: 2175 1299 525 BstXI CCAn_nnnn'nTGG Cuts at: 0 2436 3610 3999 Size: 2436 1174 389 Fragments arranged by size: 2436 1174 389 BstYI r'GATC_y Cuts at: 0 60 612 848 1223 2616 3686 3914 3999 Size: 60 552 236 375 1393 1070 228 85 Fragments arranged by size: 1393 1070 552 375 236 228 85 60 BtgI C'CryG_G Cuts at: 0 324 404 2429 3099 3230 3666 3999 Size: 324 80 2025 670 131 436 333 Fragments arranged by size: 2025 670 436 333 324 131 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2487 3999 Size: 2487 1512 BtsI GCAGTG_nn' Cuts at: 0 257 376 1058 2241 2441 2801 3999 Size: 257 119 682 1183 200 360 1198 Fragments arranged by size: 1198 1183 682 360 257 200 119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2997 3032 3999 Size: 2997 35 967 Fragments arranged by size: 2997 967 35 DraIII CAC_nnn'GTG Cuts at: 0 3950 3999 Size: 3950 49 DrdI GACnn_nn'nnGTC Cuts at: 0 562 1122 3999 Size: 562 560 2877 Fragments arranged by size: 2877 562 560 EaeI y'GGCC_r Cuts at: 0 1097 1376 2214 2460 2531 3149 3999 Size: 1097 279 838 246 71 618 850 Fragments arranged by size: 1097 850 838 618 279 246 71 EagI C'GGCC_G Cuts at: 0 2460 3999 Size: 2460 1539 EarI CTCTTCn'nnn_ Cuts at: 0 1684 2371 2938 3622 3799 3886 3999 Size: 1684 687 567 684 177 87 113 Fragments arranged by size: 1684 687 684 567 177 113 87 EcoICRI GAG'CTC Cuts at: 0 2286 3160 3850 3999 Size: 2286 874 690 149 Fragments arranged by size: 2286 874 690 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 1012 1555 2158 2254 2577 3444 3503 Size: 454 558 543 603 96 323 867 59 Cuts at: 3503 3537 3999 Size: 34 462 Fragments arranged by size: 867 603 558 543 462 454 323 96 59 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 489 1233 2161 3999 Size: 489 744 928 1838 Fragments arranged by size: 1838 928 744 489 EcoO109I rG'GnC_Cy Cuts at: 0 987 2154 2749 2750 2853 3606 3999 Size: 987 1167 595 1 103 753 393 Fragments arranged by size: 1167 987 753 595 393 103 1 EcoRI G'AATT_C Cuts at: 0 861 3999 Size: 861 3138 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2565 3897 3999 Size: 2565 1332 102 Fragments arranged by size: 2565 1332 102 FseI GG_CCGG'CC Cuts at: 0 1728 3999 Size: 1728 2271 HaeII r_GCGC'y Cuts at: 0 3251 3999 Size: 3251 748 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 279 311 452 484 874 906 1037 1069 Size: 279 32 141 32 390 32 131 32 Cuts at: 1069 3254 3286 3535 3567 3999 Size: 2185 32 249 32 432 Fragments arranged by size: 2185 432 390 279 249 141 131 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2955 3999 Size: 2955 1044 HindIII A'AGCT_T Cuts at: 0 952 2875 3024 3999 Size: 952 1923 149 975 Fragments arranged by size: 1923 975 952 149 MfeI C'AATT_G Cuts at: 0 2093 3999 Size: 2093 1906 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1023 1100 2747 2959 3759 3999 Size: 640 383 77 1647 212 800 240 Fragments arranged by size: 1647 800 640 383 240 212 77 MscI TGG'CCA Cuts at: 0 1099 1378 2216 2533 3151 3999 Size: 1099 279 838 317 618 848 Fragments arranged by size: 1099 848 838 618 317 279 MslI CAynn'nnrTG Cuts at: 0 157 361 367 799 2422 2434 3665 3999 Size: 157 204 6 432 1623 12 1231 334 Fragments arranged by size: 1623 1231 432 334 204 157 12 6 MspA1I CmG'CkG Cuts at: 0 446 678 999 1747 1912 3358 3999 Size: 446 232 321 748 165 1446 641 Fragments arranged by size: 1446 748 641 446 321 232 165 NaeI GCC'GGC Cuts at: 0 1726 3999 Size: 1726 2273 NcoI C'CATG_G Cuts at: 0 404 3999 Size: 404 3595 NgoMIV G'CCGG_C Cuts at: 0 1724 3999 Size: 1724 2275 NsiI A_TGCA'T Cuts at: 0 2479 3768 3999 Size: 2479 1289 231 Fragments arranged by size: 2479 1289 231 NspI r_CATG'y Cuts at: 0 1755 2481 2502 3999 Size: 1755 726 21 1497 Fragments arranged by size: 1755 1497 726 21 PasI CC'CwG_GG Cuts at: 0 2754 3999 Size: 2754 1245 PflMI CCAn_nnn'nTGG Cuts at: 0 1430 2903 3999 Size: 1430 1473 1096 Fragments arranged by size: 1473 1430 1096 PfoI T'CCnGG_A Cuts at: 0 989 1273 3999 Size: 989 284 2726 Fragments arranged by size: 2726 989 284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 790 822 3999 Size: 790 32 3177 Fragments arranged by size: 3177 790 32 PpuMI rG'GwC_Cy Cuts at: 0 987 2154 2853 3999 Size: 987 1167 699 1146 Fragments arranged by size: 1167 1146 987 699 PspOMI G'GGCC_C Cuts at: 0 1838 2749 3999 Size: 1838 911 1250 Fragments arranged by size: 1838 1250 911 PspXI vC'TCGA_Gb Cuts at: 0 3845 3999 Size: 3845 154 PstI C_TGCA'G Cuts at: 0 185 1451 2817 3999 Size: 185 1266 1366 1182 Fragments arranged by size: 1366 1266 1182 185 PvuII CAG'CTG Cuts at: 0 678 999 3358 3999 Size: 678 321 2359 641 Fragments arranged by size: 2359 678 641 321 SacI G_AGCT'C Cuts at: 0 2288 3162 3852 3999 Size: 2288 874 690 147 Fragments arranged by size: 2288 874 690 147 SapI GCTCTTCn'nnn_ Cuts at: 0 1684 3999 Size: 1684 2315 SfcI C'TryA_G Cuts at: 0 181 456 1176 1230 1447 2813 3420 3999 Size: 181 275 720 54 217 1366 607 579 Fragments arranged by size: 1366 720 607 579 275 217 181 54 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1105 3999 Size: 1105 2894 SmaI CCC'GGG Cuts at: 0 1837 3999 Size: 1837 2162 SmlI C'TyrA_G Cuts at: 0 724 742 1399 2578 2878 3845 3999 Size: 724 18 657 1179 300 967 154 Fragments arranged by size: 1179 967 724 657 300 154 18 SphI G_CATG'C Cuts at: 0 2481 3999 Size: 2481 1518 StuI AGG'CCT Cuts at: 0 715 1270 1759 3125 3999 Size: 715 555 489 1366 874 Fragments arranged by size: 1366 874 715 555 489 StyI C'CwwG_G Cuts at: 0 404 429 1295 1694 2049 3120 3609 3999 Size: 404 25 866 399 355 1071 489 390 Fragments arranged by size: 1071 866 489 404 399 390 355 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2626 3423 3999 Size: 2626 797 576 Fragments arranged by size: 2626 797 576 TatI w'GTAC_w Cuts at: 0 371 1775 2840 3071 3999 Size: 371 1404 1065 231 928 Fragments arranged by size: 1404 1065 928 371 231 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1507 1539 1588 1620 3999 Size: 1507 32 49 32 2379 Fragments arranged by size: 2379 1507 49 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3349 3999 Size: 3349 650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 216 771 3999 Size: 216 555 3228 Fragments arranged by size: 3228 555 216 XhoI C'TCGA_G Cuts at: 0 3845 3999 Size: 3845 154 XmaI C'CCGG_G Cuts at: 0 1835 3999 Size: 1835 2164 XmnI GAAnn'nnTTC Cuts at: 0 424 3448 3999 Size: 424 3024 551 Fragments arranged by size: 3024 551 424 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BglII BmtI BsaAI BsiWI BsmBI BspMI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI EciI EcoRV FspI FspAI HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI PacI PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI