################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AclI 593 AACGTT AA^CGTT AfaI 423 GTAC GT^AC AlwI 820 GATCC GGATC(4/5) Asp700I 427 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 653 CCCGGG C^[CT]CG[GA]G BalI 557 TGGCCA TGG^CCA BbsI 726 GTCTTC GAAGAC(2/6) BfaI 662 CTAG C^TAG BpmI 456 CTGGAG CTGGAG(16/14) BpuAI 726 GTCTTC GAAGAC(2/6) BseRI 547 GAGGAG GAGGAG(10/8) BsiYI 495 CCAGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 495 CCAGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 25 GAGAC GTCTC(1/5) BsoBI 653 CCCGGG C^[CT]CG[GA]G BsrDI 561 CATTGC GCAATG(2/0) BstF5I 263 GGATG GGATG(2/0) BstNI 356 CCAGG CC^[AT]GG DdeI 779 CTCAG C^T[ACGT]AG DraI 223 TTTAAA TTT^AAA DraIII 686 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 436 CCATGG C^C[GA][CT]GG EaeI 557 TGGCCA [CT]^GGCC[GA] EcoRII 356 CCAGG ^CC[AT]GG EcoT14I 436 CCATGG C^C[AT][AT]GG FokI 263 GGATG GGATG(9/13) HaeIII 558 GGCC GG^CC HapII 654 CCGG C^CGG HindIII 830 AAGCTT A^AGCTT HinfI 784 GACTC G^A[ACGT]TC HpaII 654 CCGG C^CGG MaeI 662 CTAG C^TAG MaeII 594 ACGT A^CGT MluNI 557 TGGCCA TGG^CCA MscI 557 TGGCCA TGG^CCA MslI 604 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 646 CAGCTG C[AC]G^C[GT]G MspI 654 CCGG C^CGG MvaI 356 CCAGG CC^[AT]GG MwoI 81 GCCTAAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 436 CCATGG C^CATGG NlaIII 437 CATG CATG^ PflFI 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 784 GACTC GAGTC(4/5) Psp1406I 593 AACGTT AA^CGTT PvuII 646 CAGCTG CAG^CTG RsaI 423 GTAC GT^AC SfcI 516 CTATAG C^T[GA][CT]AG SmaI 653 CCCGGG CCC^GGG SmlI 827 CTCAAG C^T[CT][GA]AG StyI 436 CCATGG C^C[AT][AT]GG Tth111I 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 653 CCCGGG C^CCGGG XmaI 653 CCCGGG C^CCGGG XmnI 427 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvI 272 GCTGC GCAGC(8/12) 648 GCTGC BcnI 653 CCCGG CC^[GC]GG 654 CCGGG BsmFI 491 GGGAC GGGAC(10/14) 657 GGGAC Cac8I 129 GCAAGC GC[ACGT]^[ACGT]GC 533 GCAGGC DpnII 71 GATC ^GATC 820 GATC DraII 180 AGGACCT [GA]G^G[ACGT]CC[CT] 657 GGGACCT EcoO109I 180 AGGACCT [GA]G^G[ACGT]CC[CT] 657 GGGACCT Fnu4HI 272 GCTGC GC^[ACGT]GC 648 GCTGC HgaI 105 GCGTC GACGC(5/10) 201 GCGTC HphI 762 GGTGA GGTGA(8/7) 817 GGTGA ItaI 272 GCTGC GC^[ACGT]GC 648 GCTGC MaeIII 40 GTCAC ^GT[ACGT]AC 763 GTGAC MboI 71 GATC ^GATC 820 GATC NciI 653 CCCGG CC^[GC]GG 654 CCGGG NdeII 71 GATC ^GATC 820 GATC NlaIV 491 GGGACC GG[ACGT]^[ACGT]CC 657 GGGACC PpuMI 180 AGGACCT [GA]G^G[AT]CC[CT] 657 GGGACCT Sau3AI 71 GATC ^GATC 820 GATC TseI 272 GCTGC G^C[AT]GC 648 GCTGC Tsp45I 40 GTCAC ^GT[GC]AC 763 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AcsI AluI ApoI AvaII BsaJI BsrI BssKI Cfr13I MboII MnlI MseI Sau96I ScrFI Tru9I Tsp509I TspRI