################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 204 GACGTC GACGT^C AocI 704 CCTTAGG CC^T[ACGT]AGG Aor51HI 637 AGCGCT AGC^GCT Asp700I 320 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 555 GGATCC G^GATCC BanI 163 GGTGCC G^G[CT][GA]CC BbsI 862 GAAGAC GAAGAC(2/6) BciVI 786 GGATAC GTATCC(6/5) BpuAI 862 GAAGAC GAAGAC(2/6) BseRI 37 GAGGAG GAGGAG(10/8) BstXI 437 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 704 CCTTAGG CC^T[ACGT]AGG CfoI 638 GCGC GCG^C Cfr13I 498 GGCCC G^G[ACGT]CC Eco47III 637 AGCGCT AGC^GCT Eco81I 704 CCTTAGG CC^T[ACGT]AGG FauI 500 CCCGC CCCGC(4/6) HaeII 637 AGCGCT [GA]GCGC^[CT] HgaI 7 GCGTC GACGC(5/10) HhaI 638 GCGC GCG^C HinP1I 638 GCGC G^CGC HinfI 232 GATTC G^A[ACGT]TC HphI 802 GGTGA GGTGA(8/7) MaeII 205 ACGT A^CGT MboII 862 GAAGA GAAGA(8/7) NspI 866 ACATGC [GA]CATG^[CT] PflFI 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 106 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 818 GAGCTC GAGCT^C Sau96I 498 GGCCC G^G[ACGT]CC SexAI 80 ACCTGGT A^CC[AT]GGT TaqI 458 TCGA T^CGA TfiI 232 GATTC G^A[AT]TC Tth111I 79 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 458 TCGA T^CGA Van91I 106 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 542 CCATTGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 320 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 286 GAATTT [GA]^AATT[CT] 381 AAATTT AcyI 6 GGCGTC G[GA]^CG[CT]C 204 GACGTC ApoI 286 GAATTT [GA]^AATT[CT] 381 AAATTT AspHI 667 GTGCTC G[AT]GC[AT]^C 818 GAGCTC BanII 63 GGGCTC G[GA]GC[CT]^C 818 GAGCTC BsaHI 6 GGCGTC G[GA]^CG[CT]C 204 GACGTC BsiHKAI 667 GTGCTC G[AT]GC[AT]^C 818 GAGCTC BstF5I 123 CATCC GGATG(2/0) 718 GGATG Cac8I 452 GCCAGC GC[ACGT]^[ACGT]GC 499 GCCCGC FokI 123 CATCC GGATG(9/13) 718 GGATG Hin1I 6 GGCGTC G[GA]^CG[CT]C 204 GACGTC MaeIII 732 GTCAC ^GT[ACGT]AC 803 GTGAC MseI 262 TTAA T^TAA 616 TTAA MwoI 640 GCTGCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 649 GCTGGGGAGGC NcoI 126 CCATGG C^CATGG 209 CCATGG PstI 641 CTGCAG CTGCA^G 911 CTGCAG SfaNI 307 GATGC GCATC(5/9) 646 GATGC Tru9I 262 TTAA T^TAA 616 TTAA Tsp45I 732 GTCAC ^GT[GC]AC 803 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwI AlwNI BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstNI BstYI DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI HaeIII ItaI MboI MflI MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI SfcI StyI TseI Tsp509I TspRI XhoII