(Circular) (Six-base) MAPSORT of: pFN21AE3129.seq Check: 4494 from: 1 to: 5671 pFN21AE3129 5671 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2213 3481 278 Size: 53 83 186 936 677 1268 2468 Fragments arranged by size: 2468 1268 936 677 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 3734 5118 5386 1632 Size: 1107 995 1384 268 1917 Fragments arranged by size: 1917 1384 1107 995 268 AfeI AGC'GCT Cuts at: 2644 2644 Size: 5671 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2552 Size: 32 5639 AlwNI CAG_nnn'CTG Cuts at: 2327 2543 2654 2750 3414 4986 2327 Size: 216 111 96 664 1572 3012 Fragments arranged by size: 3012 1572 664 216 111 96 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2291 2386 2980 3206 3358 5467 1493 Size: 798 95 594 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 594 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 BamHI G'GATC_C Cuts at: 2560 3001 3368 2560 Size: 441 367 4863 Fragments arranged by size: 4863 441 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 2992 4447 618 Size: 325 217 627 381 824 1455 1842 Fragments arranged by size: 1842 1455 824 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2827 2990 1391 Size: 681 755 163 4072 Fragments arranged by size: 4072 755 681 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2874 928 Size: 971 975 3725 Fragments arranged by size: 3725 975 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2785 3563 4773 2785 Size: 778 1210 3683 Fragments arranged by size: 3683 1210 778 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4282 136 Size: 107 122 71 631 605 337 2273 1525 Fragments arranged by size: 2273 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 3732 4888 1790 Size: 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 3402 4360 Size: 416 53 83 399 24 1143 842 958 Cuts at: 4360 442 Size: 1753 Fragments arranged by size: 1753 1143 958 842 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 2400 2457 4331 1239 Size: 900 261 57 1874 2579 Fragments arranged by size: 2579 1874 900 261 57 Bpu10I CC'TnA_GC Cuts at: 2751 2751 Size: 5671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2210 3478 3860 Size: 53 83 186 936 479 198 1268 382 Cuts at: 3860 4495 275 Size: 635 1451 Fragments arranged by size: 1451 1268 936 635 479 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 1186 Size: 871 4800 BseYI C'CCAG_C Cuts at: 2654 4874 5370 2654 Size: 2220 496 2955 Fragments arranged by size: 2955 2220 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2676 2827 3732 3817 4888 2676 Size: 151 905 85 1071 3459 Fragments arranged by size: 3459 1071 905 151 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2676 2827 2990 3732 3817 4888 Size: 399 282 604 151 163 742 85 1071 Cuts at: 4888 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 604 399 282 163 151 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 2254 2547 2605 2892 4166 4340 Size: 1183 963 43 293 58 287 1274 174 Cuts at: 4340 65 Size: 1396 Fragments arranged by size: 1396 1274 1183 963 293 287 174 58 43 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2449 2449 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2407 2560 2882 3001 3368 3753 3770 5222 Size: 435 153 322 119 367 385 17 1452 Cuts at: 5222 5308 5320 5666 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 435 385 367 346 322 153 119 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2710 2710 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 2004 2131 2214 2796 513 Size: 552 939 127 83 582 3388 Fragments arranged by size: 3388 939 582 552 127 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2039 3187 3981 4001 2039 Size: 1148 794 20 3709 Fragments arranged by size: 3709 1148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3118 4007 5636 Size: 54 1425 50 275 18 1288 889 1629 Cuts at: 5636 8 Size: 43 Fragments arranged by size: 1629 1425 1288 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2825 2825 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2400 2457 2739 3734 4331 5118 Size: 393 507 261 57 282 995 597 787 Cuts at: 5118 5386 1239 Size: 268 1524 Fragments arranged by size: 1524 995 787 597 507 393 282 268 261 57 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2646 4818 1678 Size: 968 2172 2531 Fragments arranged by size: 2531 2172 968 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 2430 2462 4312 Size: 32 135 32 540 32 286 32 1850 Cuts at: 4312 4344 4386 4418 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1850 540 286 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3849 Fragments arranged by size: 3849 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 3988 4147 518 Size: 648 2463 359 159 2042 Fragments arranged by size: 2463 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3087 3764 4912 5157 1606 Size: 1481 677 1148 245 2120 Fragments arranged by size: 2120 1481 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2131 2214 513 Size: 552 939 127 83 3970 Fragments arranged by size: 3970 939 552 127 83 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2875 3029 4574 1132 Size: 97 1646 154 1545 2229 Fragments arranged by size: 2229 1646 1545 154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 2117 1108 Size: 1009 4662 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 2650 2920 3023 830 Size: 1820 270 103 3478 Fragments arranged by size: 3478 1820 270 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 SacI G_AGCT'C Cuts at: 2827 2827 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SexAI A'CCwGG_T Cuts at: 2085 2085 Size: 5671 SfcI C'TryA_G Cuts at: 826 1045 2286 2319 2646 2916 3019 4154 4835 Size: 219 1241 33 327 270 103 1135 681 Cuts at: 4835 5026 826 Size: 191 1471 Fragments arranged by size: 1471 1241 1135 681 327 270 219 191 103 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3775 4676 4938 820 Size: 197 926 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2004 2131 2214 2862 3110 513 Size: 552 939 127 83 648 248 3074 Fragments arranged by size: 3074 939 648 552 248 127 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 3698 3715 3868 4053 4498 325 Size: 1255 516 1602 17 153 185 445 1498 Fragments arranged by size: 1602 1498 1255 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2715 2747 5631 5663 2715 Size: 32 2884 32 2723 Fragments arranged by size: 2884 2723 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2087 1316 Size: 771 4900 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 2554 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 2329 3800 2329 Size: 1471 4200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2211 3479 276 Size: 53 83 186 936 677 1268 2468 Fragments arranged by size: 2468 1268 936 677 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI