################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 1222 CGCG CG^CG AhdI 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 124 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 660 CCTGAGG CC^T[ACGT]AGG AspEI 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1560 GAGCTC G[AT]GC[AT]^C BbvCI 557 CCTCAGC CCTCAGC(-5/-2) BciVI 57 GGATAC GTATCC(6/5) BfaI 118 CTAG C^TAG BglI 432 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 249 GCTGAGC GC^T[ACGT]AGC Bpu1102I 249 GCTGAGC GC^T[ACGT]AGC BsaI 1507 GGTCTC GGTCTC(1/5) BsgI 462 CTGCAC GTGCAG(16/14) BsiEI 273 CGGCCG CG[GA][CT]^CG BsiHKAI 1560 GAGCTC G[AT]GC[AT]^C BstAPI 280 GCACTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1222 CGCG CG^CG BstYI 126 GGATCT [GA]^GATC[CT] Bsu36I 660 CCTGAGG CC^T[ACGT]AGG CelII 249 GCTGAGC GC^T[ACGT]AGC EagI 273 CGGCCG C^GGCCG Eam1105I 256 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 273 CGGCCG C^GGCCG Eco52I 273 CGGCCG C^GGCCG Eco81I 660 CCTGAGG CC^T[ACGT]AGG EcoT14I 937 CCTTGG C^C[AT][AT]GG HincII 571 GTCAAC GT[CT]^[GA]AC HindII 571 GTCAAC GT[CT]^[GA]AC MaeI 118 CTAG C^TAG MaeII 1380 ACGT A^CGT MflI 126 GGATCT [GA]^GATC[CT] MvnI 1222 CGCG CG^CG PaeR7I 483 CTCGAG C^TCGAG PpuMI 81 AGGACCC [GA]G^G[AT]CC[CT] PstI 642 CTGCAG CTGCA^G SacI 1560 GAGCTC GAGCT^C SexAI 837 ACCAGGT A^CC[AT]GGT SmaI 85 CCCGGG CCC^GGG SphI 1016 GCATGC GCATG^C StuI 229 AGGCCT AGG^CCT StyI 937 CCTTGG C^C[AT][AT]GG XcmI 1470 CCAGAAGGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 483 CTCGAG C^TCGAG XhoII 126 GGATCT [GA]^GATC[CT] XmaCI 85 CCCGGG C^CCGGG XmaI 85 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 126 GGATC GGATC(4/5) 907 GGATC BalI 1283 TGGCCA TGG^CCA 1482 TGGCCA BbsI 64 GAAGAC GAAGAC(2/6) 1137 GAAGAC BpuAI 64 GAAGAC GAAGAC(2/6) 1137 GAAGAC BspMI 943 ACCTGC ACCTGC(4/8) 1426 ACCTGC BstF5I 1308 CATCC GGATG(2/0) 1619 CATCC BstXI 360 CCAGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1115 CCAACCACCTGG DpnII 127 GATC ^GATC 908 GATC EarI 145 GAAGAG CTCTTC(1/4) 151 GAAGAG FokI 1308 CATCC GGATG(9/13) 1619 CATCC HaeII 1010 GGCGCT [GA]GCGC^[CT] 1463 AGCGCC HgaI 1223 GCGTC GACGC(5/10) 1296 GCGTC Ksp632I 145 GAAGAG CTCTTC(1/4) 151 GAAGAG MboI 127 GATC ^GATC 908 GATC MluNI 1283 TGGCCA TGG^CCA 1482 TGGCCA MscI 1283 TGGCCA TGG^CCA 1482 TGGCCA NdeII 127 GATC ^GATC 908 GATC Sau3AI 127 GATC ^GATC 908 GATC SmlI 483 CTCGAG C^T[CT][GA]AG 1291 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI ApaI Asp700I AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII DraIII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I Van91I XmnI