(Circular) (Six-base) MAPSORT of: pFN21AE6448.seq Check: 4914 from: 1 to: 6328 pFN21AE6448 6328 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3440 3440 Size: 6328 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2519 2820 4391 5775 6043 1632 Size: 456 431 301 1571 1384 268 1917 Fragments arranged by size: 1917 1571 1384 456 431 301 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 2266 5057 2266 Size: 2791 3537 AleI CACnn'nnGTG Cuts at: 1166 2474 3153 3321 1166 Size: 1308 679 168 4173 Fragments arranged by size: 4173 1308 679 168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6328 AlwNI CAG_nnn'CTG Cuts at: 2134 4071 5643 2134 Size: 1937 1572 2819 Fragments arranged by size: 2819 1937 1572 ApaI G_GGCC'C Cuts at: 2098 2430 2697 3060 3068 3538 3578 2098 Size: 332 267 363 8 470 40 4848 Fragments arranged by size: 4848 470 363 332 267 40 8 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 3637 3863 4015 6124 1493 Size: 2144 226 152 2109 1697 Fragments arranged by size: 2144 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2488 3570 3653 1870 Size: 73 147 398 1082 83 4545 Fragments arranged by size: 4545 1082 398 147 83 73 BamHI G'GATC_C Cuts at: 3658 4025 3658 Size: 367 5961 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 2849 3044 3219 3649 Size: 325 217 627 998 64 195 175 430 Cuts at: 3649 5104 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 998 627 430 325 217 195 175 64 BanII G_rGCy'C Cuts at: 1391 2098 2430 2697 3060 3068 3538 3569 3578 Size: 707 332 267 363 8 470 31 9 Cuts at: 3578 3647 1391 Size: 69 4072 Fragments arranged by size: 4072 707 470 363 332 267 69 31 9 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2076 3149 928 Size: 971 177 1073 4107 Fragments arranged by size: 4107 1073 971 177 BbvCI CC'TCA_GC Cuts at: 2563 2563 Size: 6328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4519 4553 1903 Size: 34 50 34 2498 34 3678 Fragments arranged by size: 3678 2498 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2056 4220 5430 2056 Size: 2164 1210 2954 Fragments arranged by size: 2954 2164 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2443 4939 136 Size: 107 122 71 631 605 771 2496 1525 Fragments arranged by size: 2496 1525 771 631 605 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 2255 3745 2255 Size: 1490 4838 Bme1580I G_kGCm'C Cuts at: 1790 2098 2287 2430 2697 2790 3060 3068 3260 Size: 308 189 143 267 93 270 8 192 Cuts at: 3260 3538 3578 4389 5545 1790 Size: 278 40 811 1156 2573 Fragments arranged by size: 2573 1156 811 308 278 270 267 192 189 143 93 40 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2754 3063 3463 Size: 416 53 83 399 24 1337 309 400 Cuts at: 3463 4059 5017 442 Size: 596 958 1753 Fragments arranged by size: 1753 1337 958 596 416 400 399 309 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2993 3057 4988 1239 Size: 917 837 64 1931 2579 Fragments arranged by size: 2579 1931 917 837 64 Bpu10I CC'TnA_GC Cuts at: 2563 2563 Size: 6328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3281 4453 5318 5616 3281 Size: 1172 865 298 3993 Fragments arranged by size: 3993 1172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3518 4991 882 Size: 617 2019 1473 2219 Fragments arranged by size: 2219 2019 1473 617 BsaAI yAC'GTr Cuts at: 493 1204 4119 493 Size: 711 2915 2702 Fragments arranged by size: 2915 2702 711 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4761 5159 5433 5580 5991 4761 Size: 398 274 147 411 5098 Fragments arranged by size: 5098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6103 Fragments arranged by size: 6103 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2174 2324 2694 2990 3042 1186 Size: 973 15 150 370 296 52 4472 Fragments arranged by size: 4472 973 370 296 150 52 15 BseYI C'CCAG_C Cuts at: 2356 2568 2995 3531 5531 6027 2356 Size: 212 427 536 2000 496 2657 Fragments arranged by size: 2657 2000 536 496 427 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2452 1163 Size: 573 716 5039 Fragments arranged by size: 5039 716 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2281 3778 4539 5567 1540 Size: 275 186 280 1497 761 1028 2301 Fragments arranged by size: 2301 1497 1028 761 280 275 186 BsiHKAI G_wGCw'C Cuts at: 3569 4389 4474 5545 3569 Size: 820 85 1071 4352 Fragments arranged by size: 4352 1071 820 85 BsmI GAATG_Cn' Cuts at: 3839 3932 3839 Size: 93 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2287 2430 2697 2790 3060 3068 Size: 399 308 189 143 267 93 270 8 Cuts at: 3068 3260 3538 3569 3578 3647 4389 4474 5545 Size: 192 278 31 9 69 742 85 1071 Cuts at: 5545 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 308 278 270 267 192 189 143 93 85 69 31 9 8 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 3440 3683 844 Size: 2113 483 243 3489 Fragments arranged by size: 3489 2113 483 243 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4823 4997 65 Size: 1183 3575 174 1396 Fragments arranged by size: 3575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4972 5159 1700 Size: 110 3162 187 2869 Fragments arranged by size: 3162 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 4388 5400 4388 Size: 1012 5316 BstAPI GCAn_nnn'nTGC Cuts at: 794 2291 794 Size: 1497 4831 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstXI CCAn_nnnn'nTGG Cuts at: 2372 3127 2372 Size: 755 5573 BstYI r'GATC_y Cuts at: 1972 2131 3658 4025 4410 4427 5879 5965 5977 Size: 159 1527 367 385 17 1452 86 12 Cuts at: 5977 6323 1972 Size: 346 1977 Fragments arranged by size: 1977 1527 1452 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2666 2666 Size: 6328 BtgI C'CryG_G Cuts at: 513 1065 2441 3444 3490 513 Size: 552 1376 1003 46 3351 Fragments arranged by size: 3351 1376 1003 552 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 2632 2921 3844 4638 4658 2632 Size: 289 923 794 20 4302 Fragments arranged by size: 4302 923 794 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DraIII CAC_nnn'GTG Cuts at: 2291 2846 3152 2291 Size: 555 306 5467 Fragments arranged by size: 5467 555 306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 2545 3288 Size: 54 1425 50 275 18 448 267 743 Cuts at: 3288 3487 3775 4664 6293 8 Size: 199 288 889 1629 43 Fragments arranged by size: 1629 1425 889 743 448 288 275 267 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2278 3775 1537 Size: 275 466 1497 4090 Fragments arranged by size: 4090 1497 466 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2151 4258 1353 Size: 792 6 2107 3423 Fragments arranged by size: 3423 2107 792 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4915 5289 5435 387 Size: 1064 138 3326 374 146 1280 Fragments arranged by size: 3326 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3567 3567 Size: 6328 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2156 2519 2820 2993 3057 4391 Size: 393 456 68 363 301 173 64 1334 Cuts at: 4391 4988 5775 6043 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1334 787 597 456 393 363 301 268 173 68 64 EcoO109I rG'GnC_Cy Cuts at: 2087 2094 2095 2426 2427 2693 2694 2896 3056 Size: 7 1 331 1 266 1 202 160 Cuts at: 3056 3057 3064 3065 3506 3535 3574 2087 Size: 1 7 1 441 29 39 4841 Fragments arranged by size: 4841 441 331 266 202 160 39 29 7 7 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 3637 4015 3637 Size: 378 5950 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 3019 3472 5475 1678 Size: 1341 453 2003 2531 Fragments arranged by size: 2531 2003 1341 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2659 2691 4969 Size: 32 135 32 680 32 375 32 2278 Cuts at: 4969 5001 5043 5075 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2278 680 375 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 3672 3918 669 Size: 988 281 640 1094 246 3079 Fragments arranged by size: 3079 1094 988 640 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5442 5626 1305 Size: 4137 184 2007 Fragments arranged by size: 4137 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3290 3489 10 Size: 54 1425 343 1458 199 2849 Fragments arranged by size: 2849 1458 1425 343 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 3153 3321 4286 4645 4804 518 Size: 648 1308 679 168 965 359 159 2042 Fragments arranged by size: 2042 1308 965 679 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2368 2523 2541 2550 2625 2730 2878 3171 Size: 762 155 18 9 75 105 148 293 Cuts at: 3171 3180 3360 3423 3744 4421 5569 5814 1606 Size: 9 180 63 321 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 321 293 245 180 155 148 105 75 63 18 9 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5776 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 2512 3025 3394 3686 5231 1132 Size: 97 1283 513 369 292 1545 2229 Fragments arranged by size: 2229 1545 1283 513 369 292 97 PasI CC'CwG_GG Cuts at: 1348 3061 3586 1348 Size: 1713 525 4090 Fragments arranged by size: 4090 1713 525 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 2191 2846 2870 1108 Size: 1026 57 655 24 4566 Fragments arranged by size: 4566 1026 655 57 24 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2519 2551 4397 4429 5910 5942 2519 Size: 32 1846 32 1481 32 2905 Fragments arranged by size: 2905 1846 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 2087 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PspOMI G'GGCC_C Cuts at: 2094 2426 2693 3056 3064 3534 3574 2094 Size: 332 267 363 8 470 40 4848 Fragments arranged by size: 4848 470 363 332 267 40 8 PspXI vC'TCGA_Gb Cuts at: 2488 2488 Size: 6328 PstI C_TGCA'G Cuts at: 830 2651 3680 830 Size: 1821 1029 3478 Fragments arranged by size: 3478 1821 1029 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 2368 2878 3360 2368 Size: 510 482 5336 Fragments arranged by size: 5336 510 482 SacI G_AGCT'C Cuts at: 3569 3569 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SexAI A'CCwGG_T Cuts at: 2842 2842 Size: 6328 SfcI C'TryA_G Cuts at: 826 1045 2647 3088 3355 3676 4811 5492 5683 Size: 219 1602 441 267 321 1135 681 191 Cuts at: 5683 826 Size: 1471 Fragments arranged by size: 1602 1471 1135 681 441 321 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 2092 3655 1872 Size: 220 1563 4545 Fragments arranged by size: 4545 1563 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 2488 3296 4432 5333 5595 820 Size: 197 926 545 808 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 808 545 262 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3025 3686 1132 Size: 1893 661 3774 Fragments arranged by size: 3774 1893 661 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 2236 1517 Size: 719 5609 StyI C'CwwG_G Cuts at: 513 1065 2942 3767 513 Size: 552 1877 825 3074 Fragments arranged by size: 3074 1877 825 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 4355 4372 4525 4710 5155 325 Size: 1255 1035 1740 17 153 185 445 1498 Fragments arranged by size: 1740 1498 1255 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 2735 4574 Size: 274 80 33 51 1443 474 284 1839 Cuts at: 4574 6286 96 Size: 1712 138 Fragments arranged by size: 1839 1712 1443 474 284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3100 3132 6288 6320 3100 Size: 32 3156 32 3108 Fragments arranged by size: 3156 3108 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3482 3482 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 2488 1943 Size: 545 5783 XmaI C'CCGG_G Cuts at: 1870 2090 3653 1870 Size: 220 1563 4545 Fragments arranged by size: 4545 1563 220 XmnI GAAnn'nnTTC Cuts at: 2534 3164 3416 4457 2534 Size: 630 252 1041 4405 Fragments arranged by size: 4405 1041 630 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI