(Linear) (Six-base) MAPSORT of: KIEE6448.seq Check: 4110 from: 1 to: 1623 KIEE6448.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1435 1623 Size: 1435 188 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 514 815 1623 Size: 83 431 301 808 Fragments arranged by size: 808 431 301 83 AhdI GACnn_n'nnGTC Cuts at: 0 261 1623 Size: 261 1362 AleI CACnn'nnGTG Cuts at: 0 469 1148 1316 1623 Size: 469 679 168 307 Fragments arranged by size: 679 469 307 168 AlwNI CAG_nnn'CTG Cuts at: 0 129 1623 Size: 129 1494 ApaI G_GGCC'C Cuts at: 0 93 425 692 1055 1063 1533 1573 1623 Size: 93 332 267 363 8 470 40 50 Fragments arranged by size: 470 363 332 267 93 50 40 8 AvaI C'yCGr_G Cuts at: 0 85 483 1565 1623 Size: 85 398 1082 58 Fragments arranged by size: 1082 398 85 58 BanI G'GyrC_C Cuts at: 0 780 844 1039 1214 1623 Size: 780 64 195 175 409 Fragments arranged by size: 780 409 195 175 64 BanII G_rGCy'C Cuts at: 0 93 425 692 1055 1063 1533 1564 1573 Size: 93 332 267 363 8 470 31 9 Cuts at: 1573 1623 Size: 50 Fragments arranged by size: 470 363 332 267 93 50 31 9 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 71 1144 1623 Size: 71 1073 479 Fragments arranged by size: 1073 479 71 BbvCI CC'TCA_GC Cuts at: 0 558 1623 Size: 558 1065 BciVI GTATCCnnnnn_n' Cuts at: 0 51 1623 Size: 51 1572 BglI GCCn_nnn'nGGC Cuts at: 0 438 1623 Size: 438 1185 BlpI GC'TnA_GC Cuts at: 0 250 1623 Size: 250 1373 Bme1580I G_kGCm'C Cuts at: 0 93 282 425 692 785 1055 1063 1255 Size: 93 189 143 267 93 270 8 192 Cuts at: 1255 1533 1573 1623 Size: 278 40 50 Fragments arranged by size: 278 270 267 192 189 143 93 93 50 40 8 BmrI ACTGGGnnnn_n' Cuts at: 0 749 1058 1458 1623 Size: 749 309 400 165 Fragments arranged by size: 749 400 309 165 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 219 1623 Size: 219 1404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 988 1052 1623 Size: 151 837 64 571 Fragments arranged by size: 837 571 151 64 Bpu10I CC'TnA_GC Cuts at: 0 558 1623 Size: 558 1065 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1276 1623 Size: 1276 347 BsaI GGTCTCn'nnnn_ Cuts at: 0 1513 1623 Size: 1513 110 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 169 319 689 985 1037 1623 Size: 154 15 150 370 296 52 586 Fragments arranged by size: 586 370 296 154 150 52 15 BseYI C'CCAG_C Cuts at: 0 351 563 990 1526 1623 Size: 351 212 427 536 97 Fragments arranged by size: 536 427 351 212 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 1623 Size: 447 1176 BsiEI CG_ry'CG Cuts at: 0 276 1623 Size: 276 1347 BsiHKAI G_wGCw'C Cuts at: 0 1564 1623 Size: 1564 59 Bsp1286I G_dGCh'C Cuts at: 0 93 282 425 692 785 1055 1063 1255 Size: 93 189 143 267 93 270 8 192 Cuts at: 1255 1533 1564 1573 1623 Size: 278 31 9 50 Fragments arranged by size: 278 270 267 192 189 143 93 93 50 31 9 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 952 1435 1623 Size: 952 483 188 Fragments arranged by size: 952 483 188 BstAPI GCAn_nnn'nTGC Cuts at: 0 286 1623 Size: 286 1337 BstXI CCAn_nnnn'nTGG Cuts at: 0 367 1122 1623 Size: 367 755 501 Fragments arranged by size: 755 501 367 BstYI r'GATC_y Cuts at: 0 126 1623 Size: 126 1497 Bsu36I CC'TnA_GG Cuts at: 0 661 1623 Size: 661 962 BtgI C'CryG_G Cuts at: 0 436 1439 1485 1623 Size: 436 1003 46 138 Fragments arranged by size: 1003 436 138 46 BtsI GCAGTG_nn' Cuts at: 0 627 916 1623 Size: 627 289 707 Fragments arranged by size: 707 627 289 DraIII CAC_nnn'GTG Cuts at: 0 286 841 1147 1623 Size: 286 555 306 476 Fragments arranged by size: 555 476 306 286 EaeI y'GGCC_r Cuts at: 0 273 540 1283 1482 1623 Size: 273 267 743 199 141 Fragments arranged by size: 743 273 267 199 141 EagI C'GGCC_G Cuts at: 0 273 1623 Size: 273 1350 EarI CTCTTCn'nnn_ Cuts at: 0 140 146 1623 Size: 140 6 1477 Fragments arranged by size: 1477 140 6 EcoICRI GAG'CTC Cuts at: 0 1562 1623 Size: 1562 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 151 514 815 988 1052 1623 Size: 83 68 363 301 173 64 571 Fragments arranged by size: 571 363 301 173 83 68 64 EcoO109I rG'GnC_Cy Cuts at: 0 82 89 90 421 422 688 689 891 Size: 82 7 1 331 1 266 1 202 Cuts at: 891 1051 1052 1059 1060 1501 1530 1569 1623 Size: 160 1 7 1 441 29 39 54 Fragments arranged by size: 441 331 266 202 160 82 54 39 29 7 7 1 1 1 1 1 HaeII r_GCGC'y Cuts at: 0 1014 1467 1623 Size: 1014 453 156 Fragments arranged by size: 1014 453 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 654 686 1623 Size: 247 32 375 32 937 Fragments arranged by size: 937 375 247 32 32 HincII GTy'rAC Cuts at: 0 573 1623 Size: 573 1050 MscI TGG'CCA Cuts at: 0 1285 1484 1623 Size: 1285 199 139 Fragments arranged by size: 1285 199 139 MslI CAynn'nnrTG Cuts at: 0 469 1148 1316 1623 Size: 469 679 168 307 Fragments arranged by size: 679 469 307 168 MspA1I CmG'CkG Cuts at: 0 363 518 536 545 620 725 873 1166 Size: 363 155 18 9 75 105 148 293 Cuts at: 1166 1175 1355 1418 1623 Size: 9 180 63 205 Fragments arranged by size: 363 293 205 180 155 148 105 75 63 18 9 9 NspI r_CATG'y Cuts at: 0 507 1020 1389 1623 Size: 507 513 369 234 Fragments arranged by size: 513 507 369 234 PasI CC'CwG_GG Cuts at: 0 1056 1581 1623 Size: 1056 525 42 Fragments arranged by size: 1056 525 42 PflMI CCAn_nnn'nTGG Cuts at: 0 129 186 841 865 1623 Size: 129 57 655 24 758 Fragments arranged by size: 758 655 129 57 24 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 514 546 1623 Size: 514 32 1077 Fragments arranged by size: 1077 514 32 PpuMI rG'GwC_Cy Cuts at: 0 82 1623 Size: 82 1541 PspOMI G'GGCC_C Cuts at: 0 89 421 688 1051 1059 1529 1569 1623 Size: 89 332 267 363 8 470 40 54 Fragments arranged by size: 470 363 332 267 89 54 40 8 PspXI vC'TCGA_Gb Cuts at: 0 483 1623 Size: 483 1140 PstI C_TGCA'G Cuts at: 0 646 1623 Size: 646 977 PvuII CAG'CTG Cuts at: 0 363 873 1355 1623 Size: 363 510 482 268 Fragments arranged by size: 510 482 363 268 SacI G_AGCT'C Cuts at: 0 1564 1623 Size: 1564 59 SexAI A'CCwGG_T Cuts at: 0 837 1623 Size: 837 786 SfcI C'TryA_G Cuts at: 0 642 1083 1350 1623 Size: 642 441 267 273 Fragments arranged by size: 642 441 273 267 SmaI CCC'GGG Cuts at: 0 87 1623 Size: 87 1536 SmlI C'TyrA_G Cuts at: 0 483 1291 1623 Size: 483 808 332 Fragments arranged by size: 808 483 332 SphI G_CATG'C Cuts at: 0 1020 1623 Size: 1020 603 StuI AGG'CCT Cuts at: 0 231 1623 Size: 231 1392 StyI C'CwwG_G Cuts at: 0 937 1623 Size: 937 686 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 610 1623 Size: 610 1013 TatI w'GTAC_w Cuts at: 0 446 730 1623 Size: 446 284 893 Fragments arranged by size: 893 446 284 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1095 1127 1623 Size: 1095 32 496 Fragments arranged by size: 1095 496 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1477 1623 Size: 1477 146 XhoI C'TCGA_G Cuts at: 0 483 1623 Size: 483 1140 XmaI C'CCGG_G Cuts at: 0 85 1623 Size: 85 1538 XmnI GAAnn'nnTTC Cuts at: 0 529 1159 1411 1623 Size: 529 630 252 212 Fragments arranged by size: 630 529 252 212 Enzymes that do cut: AarI AcuI AhdI AleI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NspI PasI PflMI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI