##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BsrI        
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraIII      DrdI        
Eam1105I    EarI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   18 enzymes) #
###################################

AfaI           49  GTAC                 GT^AC
BcgI          347  GCATCATATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BspMI         281  ACCTGC               ACCTGC(4/8)
BsrBI         232  GAGCGG               CCGCTC(-3/-3)
DsaI          238  CCGCGG               C^C[GA][CT]GG
KspI          238  CCGCGG               CCGC^GG
NotI          234  GCGGCCGC             GC^GGCCGC
PpuMI         101  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         103  GACCCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          52  CAGCTG               CAG^CTG
RsaI           49  GTAC                 GT^AC
SacII         238  CCGCGG               CCGC^GG
SfaNI         347  GCATC                GCATC(5/9)
SmaI          124  CCCGGG               CCC^GGG
TaqI          356  TCGA                 T^CGA
TthHB8I       356  TCGA                 T^CGA
XmaCI         124  CCCGGG               C^CCGGG
XmaI          124  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI           53  AGCT                 AG^CT
              302  AGCT                
Aor51HI        21  AGCGCT               AGC^GCT
              179  AGCGCT              
ApaI          149  GGGCCC               GGGCC^C
              199  GGGCCC              
BpmI          117  CTGGAG               CTGGAG(16/14)
              298  CTGGAG              
BseRI         160  CTCCTC               GAGGAG(10/8)
               75  GAGGAG              
BsiYI          81  CCCCGAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              125  CCGGGGCACGG         
BslI           81  CCCCGAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              125  CCGGGGCACGG         
BssHII        155  GCGCGC               G^CGCGC
              330  GCGCGC              
Eco47III       21  AGCGCT               AGC^GCT
              179  AGCGCT              
HaeII          21  AGCGCT               [GA]GCGC^[CT]
              179  AGCGCT              
HgaI           14  GACGC                GACGC(5/10)
              312  GACGC               
HphI          272  TCACC                GGTGA(8/7)
              279  TCACC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiEI       
BsmFI       BsoBI       Bsp1286I    BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DraII       
EaeI        EagI        EclXI       Eco52I      EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       PleI        Sau96I      
ScrFI       TseI