(Linear) (Six-base) MAPSORT of: KIEE4075.seq Check: 5558 from: 1 to: 1074 KIEE4075.orf With 182 enzymes: SgfI * March 22, 2012 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 442 1074 Size: 442 632 AatII G_ACGT'C Cuts at: 0 161 1074 Size: 161 913 AccI GT'mk_AC Cuts at: 0 275 1074 Size: 275 799 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 197 1074 Size: 197 877 AhdI GACnn_n'nnGTC Cuts at: 0 271 1074 Size: 271 803 AleI CACnn'nnGTG Cuts at: 0 205 1074 Size: 205 869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 553 1074 Size: 553 521 AlwNI CAG_nnn'CTG Cuts at: 0 702 1074 Size: 702 372 AvaI C'yCGr_G Cuts at: 0 191 707 1074 Size: 191 516 367 Fragments arranged by size: 516 367 191 BanI G'GyrC_C Cuts at: 0 426 457 1074 Size: 426 31 617 Fragments arranged by size: 617 426 31 BanII G_rGCy'C Cuts at: 0 199 521 1074 Size: 199 322 553 Fragments arranged by size: 553 322 199 BbsI GAAGACnn'nnnn_ Cuts at: 0 153 203 444 1074 Size: 153 50 241 630 Fragments arranged by size: 630 241 153 50 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 435 469 1074 Size: 435 34 605 Fragments arranged by size: 605 435 34 BclI T'GATC_A Cuts at: 0 572 857 1074 Size: 572 285 217 Fragments arranged by size: 572 285 217 BglI GCCn_nnn'nGGC Cuts at: 0 1068 1074 Size: 1068 6 BlpI GC'TnA_GC Cuts at: 0 359 520 1074 Size: 359 161 554 Fragments arranged by size: 554 359 161 Bme1580I G_kGCm'C Cuts at: 0 431 462 1074 Size: 431 31 612 Fragments arranged by size: 612 431 31 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 114 795 1074 Size: 114 681 279 Fragments arranged by size: 681 279 114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 109 691 790 823 1074 Size: 79 30 582 99 33 251 Fragments arranged by size: 582 251 99 79 33 30 Bpu10I CC'TnA_GC Cuts at: 0 415 1074 Size: 415 659 BsaAI yAC'GTr Cuts at: 0 954 1074 Size: 954 120 BsaHI Gr'CG_yC Cuts at: 0 158 1074 Size: 158 916 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 577 607 1074 Size: 577 30 467 Fragments arranged by size: 577 467 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 394 601 688 733 768 1024 1045 1074 Size: 394 207 87 45 35 256 21 29 Fragments arranged by size: 394 256 207 87 45 35 29 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 261 331 1074 Size: 261 70 743 Fragments arranged by size: 743 261 70 BsiEI CG_ry'CG Cuts at: 0 904 1063 1074 Size: 904 159 11 Fragments arranged by size: 904 159 11 BsiHKAI G_wGCw'C Cuts at: 0 521 953 1074 Size: 521 432 121 Fragments arranged by size: 521 432 121 Bsp1286I G_dGCh'C Cuts at: 0 199 431 462 521 953 1074 Size: 199 232 31 59 432 121 Fragments arranged by size: 432 232 199 121 59 31 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 302 442 1074 Size: 302 140 632 Fragments arranged by size: 632 302 140 BsrBI CCG'CTC Cuts at: 0 1059 1074 Size: 1059 15 BsrDI GCAATG_nn' Cuts at: 0 558 1074 Size: 558 516 BstXI CCAn_nnnn'nTGG Cuts at: 0 87 108 1074 Size: 87 21 966 Fragments arranged by size: 966 87 21 BtgI C'CryG_G Cuts at: 0 738 809 1074 Size: 738 71 265 Fragments arranged by size: 738 265 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 516 539 550 1074 Size: 516 23 11 524 Fragments arranged by size: 524 516 23 11 BtsI GCAGTG_nn' Cuts at: 0 484 575 671 1074 Size: 484 91 96 403 Fragments arranged by size: 484 403 96 91 EaeI y'GGCC_r Cuts at: 0 901 956 1060 1074 Size: 901 55 104 14 Fragments arranged by size: 901 104 55 14 EagI C'GGCC_G Cuts at: 0 901 1060 1074 Size: 901 159 14 Fragments arranged by size: 901 159 14 EarI CTCTTCn'nnn_ Cuts at: 0 386 505 809 1001 1049 1074 Size: 386 119 304 192 48 25 Fragments arranged by size: 386 304 192 119 48 25 EcoICRI GAG'CTC Cuts at: 0 519 1074 Size: 519 555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 109 197 691 790 823 1074 Size: 79 30 88 494 99 33 251 Fragments arranged by size: 494 251 99 88 79 33 30 FspI TGC'GCA Cuts at: 0 663 1074 Size: 663 411 HaeII r_GCGC'y Cuts at: 0 703 832 995 1074 Size: 703 129 163 79 Fragments arranged by size: 703 163 129 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 257 289 1074 Size: 257 32 785 Fragments arranged by size: 785 257 32 MslI CAynn'nnrTG Cuts at: 0 205 535 580 1074 Size: 205 330 45 494 Fragments arranged by size: 494 330 205 45 MspA1I CmG'CkG Cuts at: 0 27 230 345 740 1074 Size: 27 203 115 395 334 Fragments arranged by size: 395 334 203 115 27 NcoI C'CATG_G Cuts at: 0 809 1074 Size: 809 265 NruI TCG'CGA Cuts at: 0 535 1074 Size: 535 539 PflMI CCAn_nnn'nTGG Cuts at: 0 810 1074 Size: 810 264 PfoI T'CCnGG_A Cuts at: 0 257 1074 Size: 257 817 PmlI CAC'GTG Cuts at: 0 954 1074 Size: 954 120 PstI C_TGCA'G Cuts at: 0 287 698 731 1074 Size: 287 411 33 343 Fragments arranged by size: 411 343 287 33 SacI G_AGCT'C Cuts at: 0 521 1074 Size: 521 553 SacII CC_GC'GG Cuts at: 0 741 1074 Size: 741 333 SapI GCTCTTCn'nnn_ Cuts at: 0 809 1049 1074 Size: 809 240 25 Fragments arranged by size: 809 240 25 SfcI C'TryA_G Cuts at: 0 283 694 727 1074 Size: 283 411 33 347 Fragments arranged by size: 411 347 283 33 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1068 1074 Size: 1068 6 SmaI CCC'GGG Cuts at: 0 193 709 1074 Size: 193 516 365 Fragments arranged by size: 516 365 193 SspI AAT'ATT Cuts at: 0 405 1074 Size: 405 669 StuI AGG'CCT Cuts at: 0 169 775 1074 Size: 169 606 299 Fragments arranged by size: 606 299 169 StyI C'CwwG_G Cuts at: 0 353 809 848 1074 Size: 353 456 39 226 Fragments arranged by size: 456 353 226 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 577 609 1074 Size: 577 32 465 Fragments arranged by size: 577 465 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 807 882 1074 Size: 807 75 192 Fragments arranged by size: 807 192 75 XmaI C'CCGG_G Cuts at: 0 191 707 1074 Size: 191 516 367 Fragments arranged by size: 516 367 191 ZraI GAC'GTC Cuts at: 0 159 1074 Size: 159 915 Enzymes that do cut: AarI AatII AccI AcuI AhdI AleI AlfI AlwNI AvaI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI FspI HaeII Hin4I MslI MspA1I NcoI NruI PflMI PfoI PmlI PstI SacI SacII SapI SfcI SfiI SmaI SspI StuI StyI TstI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SwaI TaqII TatI Tth111I XbaI XhoI XmnI