################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI ClaI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SacI SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 839 GGTACC G^GTACC AccII 948 CGCG CG^CG Asp718I 839 GGTACC G^GTACC BamHI 738 GGATCC G^GATCC BanI 839 GGTACC G^G[CT][GA]CC BbvCI 20 CCTCAGC CCTCAGC(-5/-2) BciVI 963 GTATCC GTATCC(6/5) BfaI 1231 CTAG C^TAG BglII 272 AGATCT A^GATCT BsaI 1894 GAGACC GGTCTC(1/5) BsaWI 207 TCCGGT [AT]^CCGG[AT] BsiEI 714 CGGCCG CG[GA][CT]^CG BspHI 794 TCATGA T^CATGA BsrDI 1065 CATTGC GCAATG(2/0) BstUI 948 CGCG CG^CG BstXI 1838 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 185 CGGACCG CG^G[AT]CCG DrdI 1000 GACCAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 714 CGGCCG C^GGCCG EclXI 714 CGGCCG C^GGCCG Eco52I 714 CGGCCG C^GGCCG FauI 851 CCCGC CCCGC(4/6) KpnI 839 GGTACC GGTAC^C MaeI 1231 CTAG C^TAG MseI 1112 TTAA T^TAA MvnI 948 CGCG CG^CG NaeI 155 GCCGGC GCC^GGC NcoI 1838 CCATGG C^CATGG NgoMIV 155 GCCGGC G^CCGGC PflMI 615 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1319 GACTC GAGTC(4/5) PshAI 618 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 640 CAGCTG CAG^CTG RcaI 794 TCATGA T^CATGA RsrII 185 CGGACCG CG^G[AT]CCG SapI 1059 GCTCTTC GCTCTTC(1/4) SfiI 1039 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1178 CCCGGG CCC^GGG SphI 1445 GCATGC GCATG^C SspI 293 AATATT AAT^ATT Tru9I 1112 TTAA T^TAA Tsp509I 1114 AATT ^AATT Van91I 615 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1178 CCCGGG C^CCGGG XmaI 1178 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 63 GTCTAC GT^[AC][GT]AC 1637 GTCTAC AlwNI 509 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 616 CAGACACTG BalI 227 TGGCCA TGG^CCA 248 TGGCCA BbsI 1138 GAAGAC GAAGAC(2/6) 790 GTCTTC BcnI 1178 CCCGG CC^[GC]GG 1179 CCGGG BpuAI 1138 GAAGAC GAAGAC(2/6) 790 GTCTTC BsaBI 456 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1407 GATTGGCATC BsgI 338 CTGCAC GTGCAG(16/14) 832 GTGCAG BsmAI 1826 GAGAC GTCTC(1/5) 1894 GAGAC BsmI 1652 GAATGC GAATGC(1/-1) 974 GCATTC BsrFI 155 GCCGGC [GA]^CCGG[CT] 1661 GCCGGT Cfr10I 155 GCCGGC [GA]^CCGG[CT] 1661 GCCGGT MamI 456 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1407 GATTGGCATC MluNI 227 TGGCCA TGG^CCA 248 TGGCCA MscI 227 TGGCCA TGG^CCA 248 TGGCCA NciI 1178 CCCGG CC^[GC]GG 1179 CCGGG NspI 59 GCATGT [GA]CATG^[CT] 1445 GCATGC PpuMI 286 GGGACCC [GA]G^G[AT]CC[CT] 1260 GGGACCT StuI 1047 AGGCCT AGG^CCT 1200 AGGCCT TfiI 407 GATTC G^A[AT]TC 1146 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BglI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII