(Circular) (Six-base) MAPSORT of: pFN21AE6775.seq Check: 7301 from: 1 to: 6610 pFN21AE6775 6610 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2899 3893 2899 Size: 994 5616 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4420 278 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2069 3643 3953 1937 Size: 132 1574 310 4594 Fragments arranged by size: 4594 1574 310 132 Acc65I G'GTAC_C Cuts at: 2844 3931 2844 Size: 1087 5523 AclI AA'CG_TT Cuts at: 1563 4737 5110 1563 Size: 3174 373 3063 Fragments arranged by size: 3174 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 3059 3067 3198 4673 6057 6325 1632 Size: 980 447 8 131 1475 1384 268 1917 Fragments arranged by size: 1917 1475 1384 980 447 268 131 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 5509 6486 1203 Size: 4306 977 1327 Fragments arranged by size: 4306 1327 977 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 5339 5339 Size: 6610 AleI CACnn'nnGTG Cuts at: 1166 2057 3614 1166 Size: 891 1557 4162 Fragments arranged by size: 4162 1557 891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6610 AlwNI CAG_nnn'CTG Cuts at: 2519 2626 4353 5925 2519 Size: 107 1727 1572 3204 Fragments arranged by size: 3204 1727 1572 107 ApaLI G'TGCA_C Cuts at: 4667 5823 4667 Size: 1156 5454 ApoI r'AATT_y Cuts at: 1493 3919 4145 4297 6406 1493 Size: 2426 226 152 2109 1697 Fragments arranged by size: 2426 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2639 3183 3935 1870 Size: 73 104 592 544 752 4545 Fragments arranged by size: 4545 752 592 544 104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2836 2869 2836 Size: 33 6577 BamHI G'GATC_C Cuts at: 2743 3940 4307 2743 Size: 1197 367 5046 Fragments arranged by size: 5046 1197 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2844 3931 5386 618 Size: 325 217 627 1057 1087 1455 1842 Fragments arranged by size: 1842 1455 1087 1057 627 325 217 BanII G_rGCy'C Cuts at: 1391 2136 3066 3396 3446 3929 1391 Size: 745 930 330 50 483 4072 Fragments arranged by size: 4072 930 745 483 330 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2788 3150 928 Size: 971 889 362 4388 Fragments arranged by size: 4388 971 889 362 BbvCI CC'TCA_GC Cuts at: 2026 2026 Size: 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4801 4835 1903 Size: 34 2864 34 3678 Fragments arranged by size: 3678 2864 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2979 4502 5712 2979 Size: 1523 1210 3877 Fragments arranged by size: 3877 1523 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2937 3051 3556 Size: 107 122 71 631 605 1265 114 505 Cuts at: 3556 5221 136 Size: 1665 1525 Fragments arranged by size: 1665 1525 1265 631 605 505 122 114 107 71 BglII A'GATC_T Cuts at: 2277 6605 2277 Size: 4328 2282 BlpI GC'TnA_GC Cuts at: 4027 4027 Size: 6610 Bme1580I G_kGCm'C Cuts at: 1790 2054 4671 5827 1790 Size: 264 2617 1156 2573 Fragments arranged by size: 2617 2573 1156 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 3689 4341 Size: 416 53 83 399 24 2243 29 652 Cuts at: 4341 5299 442 Size: 958 1753 Fragments arranged by size: 2243 1753 958 652 416 399 83 53 29 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6610 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2501 2501 Size: 6610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 3430 3878 5270 1239 Size: 1184 1007 448 1392 2579 Fragments arranged by size: 2579 1392 1184 1007 448 Bpu10I CC'TnA_GC Cuts at: 2026 2832 2991 2026 Size: 806 159 5645 Fragments arranged by size: 5645 806 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4735 5600 5898 4735 Size: 865 298 5447 Fragments arranged by size: 5447 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3893 5273 882 Size: 617 2394 1380 2219 Fragments arranged by size: 2394 2219 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 4401 493 Size: 711 3197 2702 Fragments arranged by size: 3197 2702 711 BsaBI GATnn'nnATC Cuts at: 2465 3416 6604 2465 Size: 951 3188 2471 Fragments arranged by size: 3188 2471 951 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4417 4799 5434 275 Size: 53 83 186 936 2884 382 635 1451 Fragments arranged by size: 2884 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2212 5043 5441 5715 5862 6273 2212 Size: 2831 398 274 147 411 2549 Fragments arranged by size: 2831 2549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6385 Fragments arranged by size: 6385 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6610 BseYI C'CCAG_C Cuts at: 2154 2712 3319 3389 3817 5813 6309 2154 Size: 558 607 70 428 1996 496 2455 Fragments arranged by size: 2455 1996 607 558 496 428 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2328 2858 1163 Size: 573 592 530 4915 Fragments arranged by size: 4915 592 573 530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2722 4060 4821 5849 1540 Size: 275 186 721 1338 761 1028 2301 Fragments arranged by size: 2301 1338 1028 761 721 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2487 2880 3601 4671 4756 5827 2024 Size: 463 393 721 1070 85 1071 2807 Fragments arranged by size: 2807 1071 1070 721 463 393 85 BsmI GAATG_Cn' Cuts at: 2979 3663 4121 4214 2979 Size: 684 458 93 5375 Fragments arranged by size: 5375 684 458 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2054 2136 2487 2880 3066 3396 Size: 399 234 30 82 351 393 186 330 Cuts at: 3396 3446 3601 3929 4671 4756 5827 1391 Size: 50 155 328 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 351 330 328 234 186 155 85 82 50 30 BspEI T'CCGG_A Cuts at: 6273 6273 Size: 6610 BspHI T'CATG_A Cuts at: 2799 4499 6229 2799 Size: 1700 1730 3180 Fragments arranged by size: 3180 1730 1700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 2899 3893 3965 844 Size: 1506 549 994 72 3489 Fragments arranged by size: 3489 1506 994 549 72 BsrBI CCG'CTC Cuts at: 1394 1823 4497 1394 Size: 429 2674 3507 Fragments arranged by size: 3507 2674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 5105 5279 65 Size: 1183 1821 2036 174 1396 Fragments arranged by size: 2036 1821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2160 3666 5254 5441 1700 Size: 110 350 1506 1588 187 2869 Fragments arranged by size: 2869 1588 1506 350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6610 BssSI C'ACGA_G Cuts at: 4670 5682 4670 Size: 1012 5598 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6610 BstBI TT'CG_AA Cuts at: 5414 5414 Size: 6610 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6610 BstXI CCAn_nnnn'nTGG Cuts at: 3850 3850 Size: 6610 BstYI r'GATC_y Cuts at: 1972 2277 2743 3031 3940 4307 4692 4709 6161 Size: 305 466 288 909 367 385 17 1452 Cuts at: 6161 6247 6259 6605 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 909 466 385 367 346 305 288 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 2703 3843 513 Size: 552 1185 453 1140 3280 Fragments arranged by size: 3280 1185 1140 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5871 Fragments arranged by size: 5871 683 56 BtsI GCAGTG_nn' Cuts at: 2520 2898 3748 4126 4920 4940 2520 Size: 378 850 378 794 20 4190 Fragments arranged by size: 4190 850 794 378 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3601 3636 529 Size: 35 3037 35 3503 Fragments arranged by size: 3503 3037 35 35 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3011 5617 809 Size: 507 1695 2606 1802 Fragments arranged by size: 2606 1802 1695 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2253 2719 Size: 54 1425 50 275 18 402 21 466 Cuts at: 2719 3763 4057 4946 6575 8 Size: 1044 294 889 1629 43 Fragments arranged by size: 1629 1425 1044 889 466 402 294 275 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2719 4057 1537 Size: 275 907 1338 4090 Fragments arranged by size: 4090 1338 907 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3071 3086 3332 4540 1353 Size: 1718 15 246 1208 3423 Fragments arranged by size: 3423 1718 1208 246 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2204 5197 5571 5717 387 Size: 1064 138 615 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 615 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2612 3059 3067 3198 3430 3878 Size: 393 791 189 447 8 131 232 448 Cuts at: 3878 4673 5270 6057 6325 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 795 791 787 597 448 447 393 268 232 189 131 8 EcoO109I rG'GnC_Cy Cuts at: 2292 3043 3266 2292 Size: 751 223 5636 Fragments arranged by size: 5636 751 223 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FspI TGC'GCA Cuts at: 5116 5116 Size: 6610 HaeII r_GCGC'y Cuts at: 1678 5757 1678 Size: 4079 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3625 3657 5251 5283 5325 Size: 32 135 32 2053 32 1594 32 42 Cuts at: 5325 5357 1373 Size: 32 2626 Fragments arranged by size: 2626 2053 1594 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3954 4200 669 Size: 988 281 2016 246 3079 Fragments arranged by size: 3079 2016 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6610 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KpnI G_GTAC'C Cuts at: 2848 3935 2848 Size: 1087 5523 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5724 5908 1305 Size: 4419 184 2007 Fragments arranged by size: 4419 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2255 10 Size: 54 1425 343 402 21 4365 Fragments arranged by size: 4365 1425 402 343 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3524 3614 4568 4927 5086 518 Size: 648 891 1467 90 954 359 159 2042 Fragments arranged by size: 2042 1467 954 891 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2647 2842 3087 3316 3565 4026 4703 5851 Size: 1041 195 245 229 249 461 677 1148 Cuts at: 5851 6096 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1041 677 461 249 245 245 229 195 NaeI GCC'GGC Cuts at: 2162 2162 Size: 6610 NcoI C'CATG_G Cuts at: 513 1065 3843 513 Size: 552 2778 3280 Fragments arranged by size: 3280 2778 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6610 NgoMIV G'CCGG_C Cuts at: 2160 2160 Size: 6610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6610 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 2068 3454 3968 5513 1132 Size: 97 839 1386 514 1545 2229 Fragments arranged by size: 2229 1545 1386 839 514 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 5407 PciI A'CATG_T Cuts at: 5509 5509 Size: 6610 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 1108 Size: 1518 5092 PfoI T'CCnGG_A Cuts at: 3894 3894 Size: 6610 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4679 4711 6192 6224 4679 Size: 32 1481 32 5065 Fragments arranged by size: 5065 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2292 3266 2292 Size: 974 5636 PshAI GACnn'nnGTC Cuts at: 1613 2627 1613 Size: 1014 5596 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PstI C_TGCA'G Cuts at: 830 3279 3564 3755 3962 830 Size: 2449 285 191 207 3478 Fragments arranged by size: 3478 2449 285 207 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2647 2647 Size: 6610 RsrII CG'GwC_CG Cuts at: 2191 2191 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SanDI GG'GwC_CC Cuts at: 2292 2292 Size: 6610 SapI GCTCTTCn'nnn_ Cuts at: 3071 3071 Size: 6610 SbfI CC_TGCA'GG Cuts at: 3962 3962 Size: 6610 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SfcI C'TryA_G Cuts at: 826 1045 3275 3560 3751 3958 5093 5774 5965 Size: 219 2230 285 191 207 1135 681 191 Cuts at: 5965 826 Size: 1471 Fragments arranged by size: 2230 1471 1135 681 285 219 207 191 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3051 3051 Size: 6610 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 3185 3937 1872 Size: 1313 752 4545 Fragments arranged by size: 4545 1313 752 SmlI C'TyrA_G Cuts at: 820 1017 1943 4714 5615 5877 820 Size: 197 926 2771 901 262 1553 Fragments arranged by size: 2771 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4401 493 Size: 3908 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 3454 3968 1132 Size: 2322 514 3774 Fragments arranged by size: 3774 2322 514 SrfI GCCC'GGGC Cuts at: 3185 3185 Size: 6610 SspI AAT'ATT Cuts at: 5 52 2300 4534 5 Size: 47 2248 2234 2081 Fragments arranged by size: 2248 2234 2081 47 StuI AGG'CCT Cuts at: 1517 3054 3207 1517 Size: 1537 153 4920 Fragments arranged by size: 4920 1537 153 StyI C'CwwG_G Cuts at: 513 1065 2673 2778 3158 3334 3843 4049 513 Size: 552 1608 105 380 176 509 206 3074 Fragments arranged by size: 3074 1608 552 509 380 206 176 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2573 4637 4654 4807 4992 5437 Size: 1255 672 321 2064 17 153 185 445 Cuts at: 5437 325 Size: 1498 Fragments arranged by size: 2064 1498 1255 672 445 321 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3018 4856 6568 Size: 274 80 33 51 1443 1041 1838 1712 Cuts at: 6568 96 Size: 138 Fragments arranged by size: 1838 1712 1443 1041 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6570 6602 6570 Size: 32 6578 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6610 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2147 3619 3779 2147 Size: 1472 160 4978 Fragments arranged by size: 4978 1472 160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 3183 3935 1870 Size: 1313 752 4545 Fragments arranged by size: 4545 1313 752 XmnI GAAnn'nnTTC Cuts at: 4739 4739 Size: 6610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4418 276 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII SexAI SfoI SwaI