################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 8 CGCG CG^CG AcsI 229 AAATTC [GA]^AATT[CT] AcyI 322 GACGCC G[GA]^CG[CT]C AocI 95 CCTTAGG CC^T[ACGT]AGG ApoI 229 AAATTC [GA]^AATT[CT] Asp700I 295 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 168 GAGCAC G[AT]GC[AT]^C BamHI 392 GGATCC G^GATCC BfaI 304 CTAG C^TAG BglI 268 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 322 GACGCC G[GA]^CG[CT]C BsiHKAI 168 GAGCAC G[AT]GC[AT]^C BsmAI 129 GAGAC GTCTC(1/5) BsmFI 411 GGGAC GGGAC(10/14) BsrI 381 ACTGG ACTGG(1/-1) BssSI 236 CTCGTG CACGAG(-5/-1) BstF5I 372 GGATG GGATG(2/0) BstUI 8 CGCG CG^CG Bsu36I 95 CCTTAGG CC^T[ACGT]AGG DdeI 96 CTTAG C^T[ACGT]AG Eco81I 95 CCTTAGG CC^T[ACGT]AGG FokI 372 GGATG GGATG(9/13) HaeII 100 GGCGCT [GA]GCGC^[CT] HgaI 322 GACGC GACGC(5/10) Hin1I 322 GACGCC G[GA]^CG[CT]C HindIII 157 AAGCTT A^AGCTT HinfI 413 GACTC G^A[ACGT]TC HphI 335 TCACC GGTGA(8/7) MaeI 304 CTAG C^TAG MaeIII 355 GTTAC ^GT[ACGT]AC MfeI 252 CAATTG C^AATTG MseI 227 TTAA T^TAA MunI 252 CAATTG C^AATTG MvnI 8 CGCG CG^CG NlaIII 343 CATG CATG^ NspI 342 ACATGC [GA]CATG^[CT] PleI 413 GACTC GAGTC(4/5) SapI 165 GAAGAGC GCTCTTC(1/4) SfiI 267 GGCCCTATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 403 CTTGAG C^T[CT][GA]AG Tru9I 227 TTAA T^TAA XmnI 295 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 158 AGCT AG^CT 191 AGCT ApaI 3 GGGCCC GGGCC^C 87 GGGCCC BstNI 207 CCTGG CC^[AT]GG 264 CCAGG BstYI 149 AGATCC [GA]^GATC[CT] 392 GGATCC DpnII 150 GATC ^GATC 393 GATC EarI 361 CTCTTC CTCTTC(1/4) 165 GAAGAG EcoNI 279 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 402 CCTTGAGGAGG EcoRII 207 CCTGG ^CC[AT]GG 264 CCAGG FauI 6 CCCGC CCCGC(4/6) 84 GCGGG Ksp632I 361 CTCTTC CTCTTC(1/4) 165 GAAGAG MboI 150 GATC ^GATC 393 GATC MboII 165 GAAGA GAAGA(8/7) 362 TCTTC MflI 149 AGATCC [GA]^GATC[CT] 392 GGATCC MvaI 207 CCTGG CC^[AT]GG 264 CCAGG NdeII 150 GATC ^GATC 393 GATC Sau3AI 150 GATC ^GATC 393 GATC Tsp509I 230 AATT ^AATT 253 AATT XhoII 149 AGATCC [GA]^GATC[CT] 392 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AlwI AvaI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BssKI Cac8I CfoI Cfr13I DraII EcoO109I Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MwoI NciI NlaIV Sau96I ScrFI TseI