(Linear) (Six-base) MAPSORT of: KIEE9249.seq  Check: 6261  from: 1  to: 420



KIEE9249.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     183     420 
   Size:        145      38     237

  Fragments arranged by size: 

                 237     145      38
 
ApaI G_GGCC'C

Cuts at:      0       7      91     420 
   Size:          7      84     329

  Fragments arranged by size: 

                 329      84       7
 
ApoI r'AATT_y

Cuts at:      0     229     420 
   Size:        229     191
 
AvaI C'yCGr_G

Cuts at:      0      28      36      51     420 
   Size:         28       8      15     369

  Fragments arranged by size: 

                 369      28      15       8
 
BamHI G'GATC_C

Cuts at:      0     392     420 
   Size:        392      28
 
BanII G_rGCy'C

Cuts at:      0       7      58      91     420 
   Size:          7      51      33     329

  Fragments arranged by size: 

                 329      51      33       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     274     420 
   Size:        274     146
 
Bme1580I G_kGCm'C

Cuts at:      0       7      91     420 
   Size:          7      84     329

  Fragments arranged by size: 

                 329      84       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     376     420 
   Size:        376      44
 
BsaHI Gr'CG_yC

Cuts at:      0     323     420 
   Size:        323      97
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     120     150     420 
   Size:        120      30     270

  Fragments arranged by size: 

                 270     120      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      54     322     420 
   Size:         54     268      98

  Fragments arranged by size: 

                 268      98      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     420 
   Size:        172     248
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      58      91     172     420 
   Size:          7      51      33      81     248

  Fragments arranged by size: 

                 248      81      51      33       7
 
BssSI C'ACGA_G

Cuts at:      0     236     420 
   Size:        236     184
 
BstYI r'GATC_y

Cuts at:      0     149     392     420 
   Size:        149     243      28

  Fragments arranged by size: 

                 243     149      28
 
Bsu36I CC'TnA_GG

Cuts at:      0      96     420 
   Size:         96     324
 
EarI CTCTTCn'nnn_

Cuts at:      0     160     367     420 
   Size:        160     207      53

  Fragments arranged by size: 

                 207     160      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     141     420 
   Size:        141     279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     183     420 
   Size:        145      38     237

  Fragments arranged by size: 

                 237     145      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     283     406     420 
   Size:        283     123      14

  Fragments arranged by size: 

                 283     123      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33      87      88     267     399     420 
   Size:         33      54       1     179     132      21

  Fragments arranged by size: 

                 179     132      54      33      21       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     213     420 
   Size:        213     207
 
HaeII r_GCGC'y

Cuts at:      0     104     420 
   Size:        104     316
 
HindIII A'AGCT_T

Cuts at:      0     157     420 
   Size:        157     263
 
MfeI C'AATT_G

Cuts at:      0     252     420 
   Size:        252     168
 
NspI r_CATG'y

Cuts at:      0     346     420 
   Size:        346      74
 
PspOMI G'GGCC_C

Cuts at:      0       3      87     420 
   Size:          3      84     333

  Fragments arranged by size: 

                 333      84       3
 
SapI GCTCTTCn'nnn_

Cuts at:      0     160     420 
   Size:        160     260
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     274     420 
   Size:        274     146
 
SmlI C'TyrA_G

Cuts at:      0     403     420 
   Size:        403      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     299     420 
   Size:        299     121
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AvaI    BamHI    BanII     BglI Bme1580I
     BplI    BsaHI    BsaXI    BseRI  BsiHKAI Bsp1286I    BssSI    BstYI
   Bsu36I     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
  HindIII     MfeI     NspI   PspOMI     SapI     SfiI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI