Restriction map for
pFN21AE4046
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# NO CUT ENZYMES ( 176 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DpnII DraI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI Fnu4HI FseI FspI
HaeII HapII HhaI HinP1I HindIII
HpaII ItaI KasI KpnI Ksp632I
KspI MamI MboI MflI MluI
MluNI MroI MscI MspA1I MspI
MvnI NaeI NarI NciI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TseI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 446 GACGTC GACGT^C
AcsI 632 GAATTC [GA]^AATT[CT]
AflIII 174 ACACGT A^C[GA][CT]GT
ApoI 632 GAATTC [GA]^AATT[CT]
AseI 511 ATTAAT AT^TAAT
AsnI 511 ATTAAT AT^TAAT
Asp700I 114 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 355 GGACC G^G[AT]CC
BfaI 596 CTAG C^TAG
BpmI 500 CTGGAG CTGGAG(16/14)
BsaI 38 GGTCTC GGTCTC(1/5)
BsiYI 74 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 74 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 656 GGGAC GGGAC(10/14)
BsmI 786 GAATGC GAATGC(1/-1)
BspMI 35 GCAGGT ACCTGC(4/8)
Cac8I 330 GCTGGC GC[ACGT]^[ACGT]GC
DraII 679 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 266 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 154 GACTATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 62 CCATGG C^C[GA][CT]GG
EcoO109I 679 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 632 GAATTC G^AATTC
EcoT14I 62 CCATGG C^C[AT][AT]GG
FauI 566 GCGGG CCCGC(4/6)
HgaI 313 GACGC GACGC(5/10)
HincII 199 GTTAAC GT[CT]^[GA]AC
HindII 199 GTTAAC GT[CT]^[GA]AC
HpaI 199 GTTAAC GTT^AAC
MaeI 596 CTAG C^TAG
MaeIII 163 GTCAC ^GT[ACGT]AC
MfeI 143 CAATTG C^AATTG
MunI 143 CAATTG C^AATTG
NcoI 62 CCATGG C^CATGG
PshBI 511 ATTAAT AT^TAAT
SmlI 684 CTCAAG C^T[CT][GA]AG
SspI 376 AATATT AAT^ATT
StyI 62 CCATGG C^C[AT][AT]GG
TfiI 119 GATTC G^A[AT]TC
Tsp45I 163 GTCAC ^GT[GC]AC
XmnI 114 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 313 GACGCC G[GA]^CG[CT]C
446 GACGTC
AluI 88 AGCT AG^CT
350 AGCT
BsaHI 313 GACGCC G[GA]^CG[CT]C
446 GACGTC
BsaJI 62 CCATGG C^C[ACGT][ACGT]GG
676 CCTGGG
BsmAI 182 GAGAC GTCTC(1/5)
39 GTCTC
BsrI 279 ACTGG ACTGG(1/-1)
499 ACTGG
BstBI 242 TTCGAA TT^CGAA
635 TTCGAA
BstF5I 223 CATCC GGATG(2/0)
690 GGATG
Cfr13I 355 GGACC G^G[ACGT]CC
680 GGGCC
FokI 223 CATCC GGATG(9/13)
690 GGATG
HaeIII 6 GGCC GG^CC
681 GGCC
Hin1I 313 GACGCC G[GA]^CG[CT]C
446 GACGTC
HphI 602 TCACC GGTGA(8/7)
732 TCACC
MaeII 176 ACGT A^CGT
447 ACGT
MseI 200 TTAA T^TAA
512 TTAA
MslI 57 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
421 CATCGCTGTG
NspI 11 ACATGC [GA]CATG^[CT]
659 ACATGC
NspV 242 TTCGAA TT^CGAA
635 TTCGAA
Sau96I 355 GGACC G^G[ACGT]CC
680 GGGCC
SfuI 242 TTCGAA TT^CGAA
635 TTCGAA
TaqI 243 TCGA T^CGA
636 TCGA
Tru9I 200 TTAA T^TAA
512 TTAA
TspRI 591 CAGTG CA[GC]TG[ACGT][ACGT]^
759 CAGTG
TthHB8I 243 TCGA T^CGA
636 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI AfaI BssKI BstNI EcoRII
HinfI MboII MnlI MvaI MwoI
NlaIII NlaIV PleI RsaI ScrFI
SfaNI Tsp509I