################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AflIII 201 ACATGT A^C[GA][CT]GT Alw44I 10 GTGCAC G^TGCAC AlwNI 557 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 10 GTGCAC G^TGCAC Asp700I 834 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 10 GTGCAC G[AT]GC[AT]^C AvaI 72 CCCGGG C^[CT]CG[GA]G AvaII 31 GGACC G^G[AT]CC BalI 59 TGGCCA TGG^CCA BamHI 655 GGATCC G^GATCC BbvCI 795 GCTGAGG CCTCAGC(-5/-2) BclI 782 TGATCA T^GATCA BsaAI 46 TACGTG [CT]AC^GT[GA] BsaWI 33 ACCGGA [AT]^CCGG[AT] BsiHKAI 10 GTGCAC G[AT]GC[AT]^C BsmI 834 GAATGC GAATGC(1/-1) BsoBI 72 CCCGGG C^[CT]CG[GA]G BspLU11I 201 ACATGT A^CATGT BsrFI 894 GCCGGT [GA]^CCGG[CT] BstAPI 849 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 364 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 655 GGATCC [GA]^GATC[CT] CfoI 110 GCGC GCG^C Cfr10I 894 GCCGGT [GA]^CCGG[CT] EcoT14I 257 CCATGG C^C[AT][AT]GG FbaI 782 TGATCA T^GATCA HaeII 109 AGCGCC [GA]GCGC^[CT] HhaI 110 GCGC GCG^C HinP1I 110 GCGC G^CGC HincII 676 GTCAAC GT[CT]^[GA]AC HindII 676 GTCAAC GT[CT]^[GA]AC MflI 655 GGATCC [GA]^GATC[CT] MluNI 59 TGGCCA TGG^CCA MscI 59 TGGCCA TGG^CCA NcoI 257 CCATGG C^CATGG NspI 201 ACATGT [GA]CATG^[CT] PstI 353 CTGCAG CTGCA^G SfaNI 193 GCATC GCATC(5/9) SfcI 353 CTGCAG C^T[GA][CT]AG SmaI 72 CCCGGG CCC^GGG SmlI 187 CTTGAG C^T[CT][GA]AG StuI 368 AGGCCT AGG^CCT StyI 257 CCATGG C^C[AT][AT]GG TaqI 699 TCGA T^CGA TthHB8I 699 TCGA T^CGA XbaI 639 TCTAGA T^CTAGA XcmI 170 CCAGCAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 655 GGATCC [GA]^GATC[CT] XmaCI 72 CCCGGG C^CCGGG XmaI 72 CCCGGG C^CCGGG XmnI 834 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 607 AAATTC [GA]^AATT[CT] 851 AAATTC AlwI 656 GATCC GGATC(4/5) 655 GGATC ApaI 742 GGGCCC GGGCC^C 876 GGGCCC ApoI 607 AAATTC [GA]^AATT[CT] 851 AAATTC BfaI 640 CTAG C^TAG 803 CTAG BseRI 313 CTCCTC GAGGAG(10/8) 725 GAGGAG BsmAI 138 GAGAC GTCTC(1/5) 422 GAGAC BstF5I 360 CATCC GGATG(2/0) 485 GGATG DsaI 257 CCATGG C^C[GA][CT]GG 869 CCACGG EaeI 59 TGGCCA [CT]^GGCC[GA] 104 CGGCCA EcoNI 90 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 342 CCTGACCCAGG FokI 360 CATCC GGATG(9/13) 485 GGATG HindIII 529 AAGCTT A^AGCTT 547 AAGCTT HinfI 566 GATTC G^A[ACGT]TC 908 GAATC MaeI 640 CTAG C^TAG 803 CTAG MslI 252 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 480 CACCCGGATG TfiI 566 GATTC G^A[AT]TC 908 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I Tsp509I TspRI