Restriction map for
pFN21AE1817
(Circular) (Six-base) MAPSORT of: pFN21AE1817.seq Check: 7919 from: 1 to: 5701
pFN21AE1817 5701 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2619 3764 5148 5416 1632
Size: 488 499 1145 1384 268 1917
Fragments arranged by size:
1917 1384 1145 499 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 2206 4600 5577 1203
Size: 1003 2394 977 1327
Fragments arranged by size:
2394 1327 1003 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 2567 3444 5016 2567
Size: 877 1572 3252
Fragments arranged by size:
3252 1572 877
ApaI G_GGCC'C
Cuts at: 2751 2885 2751
Size: 134 5567
ApaLI G'TGCA_C
Cuts at: 2015 3758 4914 2015
Size: 1743 1156 2802
Fragments arranged by size:
2802 1743 1156
ApoI r'AATT_y
Cuts at: 1493 2612 2856 3010 3236 3388 5497 1493
Size: 1119 244 154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 244 226 154 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 3026 1870
Size: 73 134 949 4545
Fragments arranged by size:
4545 949 134 73
BamHI G'GATC_C
Cuts at: 2660 3031 3398 2660
Size: 371 367 4963
Fragments arranged by size:
4963 371 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 4477 618
Size: 325 217 627 1235 1455 1842
Fragments arranged by size:
1842 1455 1235 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2078 2319 2751 2885 3020 1391
Size: 687 241 432 134 135 4072
Fragments arranged by size:
4072 687 432 241 135 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BbvCI CC'TCA_GC
Cuts at: 2801 2801
Size: 5701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 2787 1548
Size: 1239 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4312 136
Size: 107 122 71 631 605 2640 1525
Fragments arranged by size:
2640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2468 2751 2885 3762 4918 1790
Size: 229 449 283 134 877 1156 2573
Fragments arranged by size:
2573 1156 877 449 283 229 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2677 2915 3432
Size: 416 53 83 399 24 1260 238 517
Cuts at: 3432 4390 442
Size: 958 1753
Fragments arranged by size:
1753 1260 958 517 416 399 238 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2445 2517 2649 4361 1239
Size: 912 294 72 132 1712 2579
Fragments arranged by size:
2579 1712 912 294 132 72
Bpu10I CC'TnA_GC
Cuts at: 2801 2801
Size: 5701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3826 4691 4989 2213
Size: 1613 865 298 2925
Fragments arranged by size:
2925 1613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3492 493
Size: 711 849 1439 2702
Fragments arranged by size:
2702 1439 849 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2038 4134 4532 4806 4953 5364 2038
Size: 2096 398 274 147 411 2375
Fragments arranged by size:
2375 2096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2418 2448 1178
Size: 30 165 30 1015 30 4431
Fragments arranged by size:
4431 1015 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 2745 1186
Size: 1123 436 4142
Fragments arranged by size:
4142 1123 436
BseYI C'CCAG_C
Cuts at: 2218 4904 5400 2218
Size: 2686 496 2519
Fragments arranged by size:
2686 2519 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 3762 3847 4918 2019
Size: 1743 85 1071 2802
Fragments arranged by size:
2802 1743 1071 85
BsmI GAATG_Cn'
Cuts at: 2845 3212 3305 2845
Size: 367 93 5241
Fragments arranged by size:
5241 367 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2078 2319 2468 2751 2885 3020
Size: 399 229 59 241 149 283 134 135
Cuts at: 3020 3762 3847 4918 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 241 229 149
135 134 85 59
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2899 4345 4532 1700
Size: 110 1089 1446 187 2869
Fragments arranged by size:
2869 1446 1089 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2860 794
Size: 2066 3635
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2376 2376
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 2660 3031 3398 3783 3800 5252 5338 5350
Size: 688 371 367 385 17 1452 86 12
Cuts at: 5350 5696 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 688 385 371 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 2874 513
Size: 552 1197 612 3340
Fragments arranged by size:
3340 1197 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2109 3148
Size: 54 1425 50 275 18 234 45 1039
Cuts at: 3148 4037 5666 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1039 889 275 234 54 50
45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3631 1353
Size: 2278 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 2673 4288 4662 4808 387
Size: 1064 138 555 529 1615 374 146 1280
Fragments arranged by size:
1615 1280 1064 555 529 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2151 2445 2517 2619 2649 3764
Size: 393 488 31 294 72 102 30 1115
Cuts at: 3764 4361 5148 5416 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1115 787 597 488 393 294 268
102 72 31 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2351 2099
Size: 252 5449
EcoO109I rG'GnC_Cy
Cuts at: 2222 2748 2881 2882 2222
Size: 526 133 1 5041
Fragments arranged by size:
5041 526 133 1
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2118 4848 1678
Size: 440 2730 2531
Fragments arranged by size:
2730 2531 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2697 2724 2729 2756 4342
Size: 32 135 32 1125 27 5 27 1586
Cuts at: 4342 4374 4416 4448 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1586 1125 135 42 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2683 3045 3291 669
Size: 988 281 745 362 246 3079
Fragments arranged by size:
3079 988 745 362 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 2552 748
Size: 1786 18 3897
Fragments arranged by size:
3897 1786 18
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2503 4815 4999 1305
Size: 1198 2312 184 2007
Fragments arranged by size:
2312 2007 1198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 10
Size: 54 1425 343 234 3645
Fragments arranged by size:
3645 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2489 3659 4018 4177 518
Size: 648 1095 228 1170 359 159 2042
Fragments arranged by size:
2042 1170 1095 648 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 2395 2471 2519 2564 3117 3794 4942 5187
Size: 789 76 48 45 553 677 1148 245
Cuts at: 5187 1606
Size: 2120
Fragments arranged by size:
2120 1148 789 677 553 245 76 48
45
NcoI C'CATG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 3952
Fragments arranged by size:
3952 1197 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2210 3059 4604 1132
Size: 97 981 849 1545 2229
Fragments arranged by size:
2229 1545 981 849 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 2206 4600 2206
Size: 2394 3307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PfoI T'CCnGG_A
Cuts at: 2167 2934 2985 2167
Size: 767 51 4883
Fragments arranged by size:
4883 767 51
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2747 2881 2747
Size: 134 5567
PstI C_TGCA'G
Cuts at: 830 2362 3053 830
Size: 1532 691 3478
Fragments arranged by size:
3478 1532 691
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
PvuII CAG'CTG
Cuts at: 2395 2471 2519 2564 2395
Size: 76 48 45 5532
Fragments arranged by size:
5532 76 48 45
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2358 3049 4184 4865 5056 826
Size: 219 1313 691 1135 681 191 1471
Fragments arranged by size:
1471 1313 1135 691 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 2079 3028 1872
Size: 207 949 4545
Fragments arranged by size:
4545 949 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3805 4706 4968 820
Size: 197 926 249 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 1517
Size: 858 4843
StyI C'CwwG_G
Cuts at: 513 1065 2262 3140 513
Size: 552 1197 878 3074
Fragments arranged by size:
3074 1197 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2942 3728 3745 3898 4083 4528 325
Size: 1255 1362 786 17 153 185 445 1498
Fragments arranged by size:
1498 1362 1255 786 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2820 2949 3947
Size: 274 80 33 51 1443 843 129 998
Cuts at: 3947 5659 96
Size: 1712 138
Fragments arranged by size:
1712 1443 998 843 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 5661 5693 2418
Size: 32 3211 32 2426
Fragments arranged by size:
3211 2426 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 2644 3037 2644
Size: 393 5308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2182
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 2077 3026 1870
Size: 207 949 4545
Fragments arranged by size:
4545 949 207
XmnI GAAnn'nnTTC
Cuts at: 2843 3830 2843
Size: 987 4714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI