(Linear) (Six-base) MAPSORT of: KIEE1817.seq Check: 5332 from: 1 to: 996 KIEE1817.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 614 996 Size: 115 499 382 Fragments arranged by size: 499 382 115 AflIII A'CryG_T Cuts at: 0 201 996 Size: 201 795 AlwNI CAG_nnn'CTG Cuts at: 0 562 996 Size: 562 434 ApaI G_GGCC'C Cuts at: 0 746 880 996 Size: 746 134 116 Fragments arranged by size: 746 134 116 ApaLI G'TGCA_C Cuts at: 0 10 996 Size: 10 986 ApoI r'AATT_y Cuts at: 0 607 851 996 Size: 607 244 145 Fragments arranged by size: 607 244 145 AvaI C'yCGr_G Cuts at: 0 72 996 Size: 72 924 BamHI G'GATC_C Cuts at: 0 655 996 Size: 655 341 BanII G_rGCy'C Cuts at: 0 73 314 746 880 996 Size: 73 241 432 134 116 Fragments arranged by size: 432 241 134 116 73 BbvCI CC'TCA_GC Cuts at: 0 796 996 Size: 796 200 BclI T'GATC_A Cuts at: 0 782 996 Size: 782 214 Bme1580I G_kGCm'C Cuts at: 0 14 463 746 880 996 Size: 14 449 283 134 116 Fragments arranged by size: 449 283 134 116 14 BmrI ACTGGGnnnn_n' Cuts at: 0 672 910 996 Size: 672 238 86 Fragments arranged by size: 672 238 86 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 606 996 Size: 606 390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 440 512 644 996 Size: 146 294 72 132 352 Fragments arranged by size: 352 294 146 132 72 Bpu10I CC'TnA_GC Cuts at: 0 796 996 Size: 796 200 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 996 Size: 208 788 BsaAI yAC'GTr Cuts at: 0 48 996 Size: 48 948 BsaWI w'CCGG_w Cuts at: 0 33 996 Size: 33 963 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 413 443 996 Size: 413 30 553 Fragments arranged by size: 553 413 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 304 740 996 Size: 304 436 256 Fragments arranged by size: 436 304 256 BseYI C'CCAG_C Cuts at: 0 213 996 Size: 213 783 BsiHKAI G_wGCw'C Cuts at: 0 14 996 Size: 14 982 BsmI GAATG_Cn' Cuts at: 0 840 996 Size: 840 156 Bsp1286I G_dGCh'C Cuts at: 0 14 73 314 463 746 880 996 Size: 14 59 241 149 283 134 116 Fragments arranged by size: 283 241 149 134 116 59 14 BsrFI r'CCGG_y Cuts at: 0 894 996 Size: 894 102 BstAPI GCAn_nnn'nTGC Cuts at: 0 855 996 Size: 855 141 BstXI CCAn_nnnn'nTGG Cuts at: 0 371 996 Size: 371 625 BstYI r'GATC_y Cuts at: 0 655 996 Size: 655 341 BtgI C'CryG_G Cuts at: 0 257 869 996 Size: 257 612 127 Fragments arranged by size: 612 257 127 EaeI y'GGCC_r Cuts at: 0 59 104 996 Size: 59 45 892 Fragments arranged by size: 892 59 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 139 668 996 Size: 139 529 328 Fragments arranged by size: 529 328 139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 146 440 512 614 644 996 Size: 115 31 294 72 102 30 352 Fragments arranged by size: 352 294 115 102 72 31 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 94 346 996 Size: 94 252 650 Fragments arranged by size: 650 252 94 EcoO109I rG'GnC_Cy Cuts at: 0 217 743 876 877 996 Size: 217 526 133 1 119 Fragments arranged by size: 526 217 133 119 1 HaeII r_GCGC'y Cuts at: 0 113 996 Size: 113 883 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 692 719 724 751 996 Size: 692 27 5 27 245 Fragments arranged by size: 692 245 27 27 5 HincII GTy'rAC Cuts at: 0 678 996 Size: 678 318 HindIII A'AGCT_T Cuts at: 0 529 547 996 Size: 529 18 449 Fragments arranged by size: 529 449 18 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 498 996 Size: 498 498 MscI TGG'CCA Cuts at: 0 61 996 Size: 61 935 MslI CAynn'nnrTG Cuts at: 0 256 484 996 Size: 256 228 512 Fragments arranged by size: 512 256 228 MspA1I CmG'CkG Cuts at: 0 390 466 514 559 996 Size: 390 76 48 45 437 Fragments arranged by size: 437 390 76 48 45 NcoI C'CATG_G Cuts at: 0 257 996 Size: 257 739 NspI r_CATG'y Cuts at: 0 205 996 Size: 205 791 PciI A'CATG_T Cuts at: 0 201 996 Size: 201 795 PfoI T'CCnGG_A Cuts at: 0 162 929 980 996 Size: 162 767 51 16 Fragments arranged by size: 767 162 51 16 PspOMI G'GGCC_C Cuts at: 0 742 876 996 Size: 742 134 120 Fragments arranged by size: 742 134 120 PstI C_TGCA'G Cuts at: 0 357 996 Size: 357 639 PvuII CAG'CTG Cuts at: 0 390 466 514 559 996 Size: 390 76 48 45 437 Fragments arranged by size: 437 390 76 48 45 SfcI C'TryA_G Cuts at: 0 353 996 Size: 353 643 SmaI CCC'GGG Cuts at: 0 74 996 Size: 74 922 SmlI C'TyrA_G Cuts at: 0 187 996 Size: 187 809 StuI AGG'CCT Cuts at: 0 370 996 Size: 370 626 StyI C'CwwG_G Cuts at: 0 257 996 Size: 257 739 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 937 996 Size: 937 59 TatI w'GTAC_w Cuts at: 0 815 944 996 Size: 815 129 52 Fragments arranged by size: 815 129 52 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 413 445 996 Size: 413 32 551 Fragments arranged by size: 551 413 32 XbaI T'CTAG_A Cuts at: 0 639 996 Size: 639 357 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 177 996 Size: 177 819 XmaI C'CCGG_G Cuts at: 0 72 996 Size: 72 924 XmnI GAAnn'nnTTC Cuts at: 0 838 996 Size: 838 158 Enzymes that do cut: AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrFI BstAPI BstXI BstYI BtgI EaeI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PciI PfoI PspOMI PstI PvuII SfcI SmaI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BsaI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI ZraI