Restriction map for
pFN21AE3893
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# NO CUT ENZYMES ( 205 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI MvaI MvnI NaeI
NarI NcoI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AflIII 64 ACGTGT A^C[GA][CT]GT
Alw44I 73 GTGCAC G^TGCAC
ApaLI 73 GTGCAC G^TGCAC
AspHI 73 GTGCAC G[AT]GC[AT]^C
BmyI 73 GTGCAC G[AGT]GC[ACT]^C
BsaI 16 GAGACC GGTCTC(1/5)
BsiHKAI 73 GTGCAC G[AT]GC[AT]^C
BsmBI 32 GAGACG CGTCTC(1/5)
BsmI 86 GAATGC GAATGC(1/-1)
Bsp1286I 73 GTGCAC G[AGT]GC[ACT]^C
BspMI 226 ACCTGC ACCTGC(4/8)
DdeI 318 CTTAG C^T[ACGT]AG
DpnII 12 GATC ^GATC
DsaI 254 CCACGG C^C[GA][CT]GG
EarI 181 GAAGAG CTCTTC(1/4)
HinfI 112 GATTC G^A[ACGT]TC
Ksp632I 181 GAAGAG CTCTTC(1/4)
MboI 12 GATC ^GATC
MwoI 244 GCATATGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 245 CATATG CA^TATG
NdeII 12 GATC ^GATC
NlaIV 250 GGAGCC GG[ACGT]^[ACGT]CC
Sau3AI 12 GATC ^GATC
TaqI 14 TCGA T^CGA
TfiI 112 GATTC G^A[AT]TC
Tsp509I 150 AATT ^AATT
TthHB8I 14 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbvI 190 GCAGC GCAGC(8/12)
309 GCTGC
BcnI 28 CCCGG CC^[GC]GG
265 CCCGG
BsgI 228 CTGCAC GTGCAG(16/14)
188 GTGCAG
BssKI 28 CCCGG ^CC[ACGT]GG
265 CCCGG
BstF5I 116 CATCC GGATG(2/0)
273 CATCC
Cfr13I 57 GGGCC G^G[ACGT]CC
258 GGGCC
Fnu4HI 190 GCAGC GC^[ACGT]GC
309 GCTGC
FokI 116 CATCC GGATG(9/13)
273 CATCC
HaeIII 58 GGCC GG^CC
259 GGCC
HapII 29 CCGG C^CGG
266 CCGG
HpaII 29 CCGG C^CGG
266 CCGG
ItaI 190 GCAGC GC^[ACGT]GC
309 GCTGC
MaeII 64 ACGT A^CGT
302 ACGT
MboII 102 GAAGA GAAGA(8/7)
181 GAAGA
MslI 81 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
262 CACCCCGGTG
MspI 29 CCGG C^CGG
266 CCGG
NciI 28 CCCGG CC^[GC]GG
265 CCCGG
Sau96I 57 GGGCC G^G[ACGT]CC
258 GGGCC
ScrFI 28 CCCGG CC^[ACGT]GG
265 CCCGG
TseI 190 GCAGC G^C[AT]GC
309 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsmAI
MnlI