################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BcgI BclI BfrI BglI BlnI BsaBI BsaI BseRI BsiEI BsiWI BsmBI BspDI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 598 GTAGAC GT^[AC][GT]AC AccII 53 CGCG CG^CG AclI 263 AACGTT AA^CGTT AcsI 276 AAATTT [GA]^AATT[CT] ApoI 276 AAATTT [GA]^AATT[CT] AseI 1612 ATTAAT AT^TAAT AsnI 1612 ATTAAT AT^TAAT AspHI 1155 GAGCAC G[AT]GC[AT]^C BbsI 2290 GAAGAC GAAGAC(2/6) BbvCI 976 GCTGAGG CCTCAGC(-5/-2) BciVI 1305 GTATCC GTATCC(6/5) BglII 174 AGATCT A^GATCT BpuAI 2290 GAAGAC GAAGAC(2/6) BsiHKAI 1155 GAGCAC G[AT]GC[AT]^C Bsp1407I 1888 TGTACA T^GTACA BspHI 1212 TCATGA T^CATGA BspLU11I 192 ACATGT A^CATGT BsrGI 1888 TGTACA T^GTACA BssSI 1330 CACGAG CACGAG(-5/-1) BstEII 2397 GGTCACC G^GT[ACGT]ACC BstPI 2397 GGTCACC G^GT[ACGT]ACC BstUI 53 CGCG CG^CG DrdI 2389 GACCATCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1080 CCATGG C^C[GA][CT]GG EaeI 1824 TGGCCG [CT]^GGCC[GA] EcoNI 1182 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2397 GGTCACC G^GT[ACGT]ACC EcoRV 511 GATATC GAT^ATC EcoT14I 1080 CCATGG C^C[AT][AT]GG EcoT22I 1731 ATGCAT ATGCA^T FauI 1665 CCCGC CCCGC(4/6) HgaI 2436 GCGTC GACGC(5/10) HincII 2449 GTTGAC GT[CT]^[GA]AC HindII 2449 GTTGAC GT[CT]^[GA]AC HindIII 100 AAGCTT A^AGCTT MvnI 53 CGCG CG^CG NcoI 1080 CCATGG C^CATGG NdeI 2311 CATATG CA^TATG NsiI 1731 ATGCAT ATGCA^T PshBI 1612 ATTAAT AT^TAAT Psp1406I 263 AACGTT AA^CGTT RcaI 1212 TCATGA T^CATGA ScaI 256 AGTACT AGT^ACT SmaI 69 CCCGGG CCC^GGG SspBI 1888 TGTACA T^GTACA StyI 1080 CCATGG C^C[AT][AT]GG XmaCI 69 CCCGGG C^CCGGG XmaI 69 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 60 TCCGGA T^CCGGA 1248 TCCGGA AcyI 48 GGCGCC G[GA]^CG[CT]C 2241 GGCGCC AlwNI 1509 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 1756 CAGCAGCTG BanI 48 GGCGCC G^G[CT][GA]CC 2241 GGCGCC BbeI 48 GGCGCC GGCGC^C 2241 GGCGCC BbrPI 352 CACGTG CAC^GTG 669 CACGTG BcnI 69 CCCGG CC^[GC]GG 70 CCGGG BlpI 1974 GCTGAGC GC^T[ACGT]AGC 2145 GCTGAGC Bpu1102I 1974 GCTGAGC GC^T[ACGT]AGC 2145 GCTGAGC BsaAI 352 CACGTG [CT]AC^GT[GA] 669 CACGTG BsaHI 48 GGCGCC G[GA]^CG[CT]C 2241 GGCGCC BseAI 60 TCCGGA T^CCGGA 1248 TCCGGA BsmFI 1221 GGGAC GGGAC(10/14) 1814 GGGAC BspEI 60 TCCGGA T^CCGGA 1248 TCCGGA BspMI 1498 ACCTGC ACCTGC(4/8) 808 GCAGGT CelII 1974 GCTGAGC GC^T[ACGT]AGC 2145 GCTGAGC DraII 1814 GGGACCT [GA]G^G[ACGT]CC[CT] 2336 AGGACCC EcoO109I 1814 GGGACCT [GA]G^G[ACGT]CC[CT] 2336 AGGACCC HaeII 48 GGCGCC [GA]GCGC^[CT] 2241 GGCGCC Hin1I 48 GGCGCC G[GA]^CG[CT]C 2241 GGCGCC KasI 48 GGCGCC G^GCGCC 2241 GGCGCC MroI 60 TCCGGA T^CCGGA 1248 TCCGGA MslI 1081 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1311 CATAGCTGTG NarI 48 GGCGCC GG^CGCC 2241 GGCGCC NciI 69 CCCGG CC^[GC]GG 70 CCGGG NspI 192 ACATGT [GA]CATG^[CT] 1774 GCATGT PaeR7I 94 CTCGAG C^TCGAG 1440 CTCGAG PflMI 2073 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2268 CCAGAGAATGG PmaCI 352 CACGTG CAC^GTG 669 CACGTG PmlI 352 CACGTG CAC^GTG 669 CACGTG PpuMI 1814 GGGACCT [GA]G^G[AT]CC[CT] 2336 AGGACCC SapI 616 GAAGAGC GCTCTTC(1/4) 641 GAAGAGC SexAI 876 ACCTGGT A^CC[AT]GGT 1199 ACCTGGT SfoI 48 GGCGCC GGC^GCC 2241 GGCGCC Van91I 2073 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2268 CCAGAGAATGG XhoI 94 CTCGAG C^TCGAG 1440 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AvaI AvaII BbvI BfaI BmyI BpmI BsaJI BsaWI BsgI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII