(Circular) (Six-base) MAPSORT of: pFN21AE1898.seq Check: 8272 from: 1 to: 7177 pFN21AE1898 7177 bp With 182 enzymes: SgfI * August 13, 2015 11:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3512 3512 Size: 7177 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 4520 1937 Size: 667 1916 4594 Fragments arranged by size: 4594 1916 667 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 2269 5304 5677 1563 Size: 706 3035 373 3063 Fragments arranged by size: 3063 3035 706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2640 3708 5240 6624 6892 1632 Size: 900 108 1068 1532 1384 268 1917 Fragments arranged by size: 1917 1532 1384 1068 900 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 2197 2356 2673 2675 6076 7053 1203 Size: 994 159 317 2 3401 977 1327 Fragments arranged by size: 3401 1327 994 977 317 159 2 AgeI A'CCGG_T Cuts at: 6008 6008 Size: 7177 AhdI GACnn_n'nnGTC Cuts at: 5906 5906 Size: 7177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7177 AlwNI CAG_nnn'CTG Cuts at: 3519 3766 4920 6492 3519 Size: 247 1154 1572 4204 Fragments arranged by size: 4204 1572 1154 247 ApaLI G'TGCA_C Cuts at: 5234 6390 5234 Size: 1156 6021 ApoI r'AATT_y Cuts at: 1493 2281 4486 4712 4864 6973 1493 Size: 788 2205 226 152 2109 1697 Fragments arranged by size: 2205 2109 1697 788 226 152 AseI AT'TA_AT Cuts at: 160 1032 3618 5731 160 Size: 872 2586 2113 1606 Fragments arranged by size: 2586 2113 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2099 3445 4502 1870 Size: 73 131 25 1346 1057 4545 Fragments arranged by size: 4545 1346 1057 131 73 25 BamHI G'GATC_C Cuts at: 4507 4874 4507 Size: 367 6810 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 4246 4498 5953 618 Size: 325 217 627 266 2193 252 1455 1842 Fragments arranged by size: 2193 1842 1455 627 325 266 252 217 BanII G_rGCy'C Cuts at: 1391 4496 1391 Size: 3105 4072 BbeI G_GCGC'C Cuts at: 2057 4250 2057 Size: 2193 4984 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4302 928 Size: 971 2403 3803 Fragments arranged by size: 3803 2403 971 BbvCI CC'TCA_GC Cuts at: 2982 2982 Size: 7177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5368 5402 1903 Size: 34 3431 34 3678 Fragments arranged by size: 3678 3431 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3321 5069 6279 3321 Size: 1748 1210 4219 Fragments arranged by size: 4219 1748 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7177 BfrBI ATG'CAT Cuts at: 3738 3738 Size: 7177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5788 136 Size: 107 122 71 631 605 4116 1525 Fragments arranged by size: 4116 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2179 7172 2179 Size: 4993 2184 BlpI GC'TnA_GC Cuts at: 3980 4151 4594 3980 Size: 171 443 6563 Fragments arranged by size: 6563 443 171 Bme1580I G_kGCm'C Cuts at: 1790 3277 3671 3843 5238 6394 1790 Size: 1487 394 172 1395 1156 2573 Fragments arranged by size: 2573 1487 1395 1156 394 172 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3113 4128 4908 Size: 416 53 83 399 24 1696 1015 780 Cuts at: 4908 5866 442 Size: 958 1753 Fragments arranged by size: 1753 1696 1015 958 780 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2392 2031 Size: 361 6816 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3660 3788 4194 5837 1239 Size: 2421 128 406 1643 2579 Fragments arranged by size: 2579 2421 1643 406 128 Bpu10I CC'TnA_GC Cuts at: 2982 2982 Size: 7177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 5302 6167 6465 2843 Size: 2459 865 298 3555 Fragments arranged by size: 3555 2459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5840 882 Size: 617 4341 2219 Fragments arranged by size: 4341 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2359 2676 4968 493 Size: 711 1155 317 2292 2702 Fragments arranged by size: 2702 2292 1155 711 317 BsaBI GATnn'nnATC Cuts at: 7171 7171 Size: 7177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 4247 4984 5366 Size: 53 83 186 936 521 2193 737 382 Cuts at: 5366 6001 275 Size: 635 1451 Fragments arranged by size: 2193 1451 936 737 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2065 3253 4399 5610 6008 6282 6429 6840 2065 Size: 1188 1146 1211 398 274 147 411 2402 Fragments arranged by size: 2402 1211 1188 1146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6952 Fragments arranged by size: 6952 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7177 BseYI C'CCAG_C Cuts at: 4301 6380 6876 4301 Size: 2079 496 4602 Fragments arranged by size: 4602 2079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3505 3565 3631 1163 Size: 573 1769 60 66 4709 Fragments arranged by size: 4709 1769 573 66 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4627 5388 6416 1540 Size: 275 186 2626 761 1028 2301 Fragments arranged by size: 2626 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3164 5238 5323 6394 3164 Size: 2074 85 1071 3947 Fragments arranged by size: 3947 2074 1071 85 BsmI GAATG_Cn' Cuts at: 2161 2853 4068 4688 4781 2161 Size: 692 1215 620 93 4557 Fragments arranged by size: 4557 1215 692 620 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3164 3277 3671 3843 4496 5238 5323 Size: 399 1374 113 394 172 653 742 85 Cuts at: 5323 6394 1391 Size: 1071 2174 Fragments arranged by size: 2174 1374 1071 742 653 399 394 172 113 85 BspEI T'CCGG_A Cuts at: 2065 3253 6840 2065 Size: 1188 3587 2402 Fragments arranged by size: 3587 2402 1188 BspHI T'CATG_A Cuts at: 3217 5066 6796 3217 Size: 1849 1730 3598 Fragments arranged by size: 3598 1849 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 3512 4532 844 Size: 1960 708 1020 3489 Fragments arranged by size: 3489 1960 1020 708 BsrBI CCG'CTC Cuts at: 1394 1823 5064 1394 Size: 429 3241 3507 Fragments arranged by size: 3507 3241 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5672 5846 65 Size: 1183 4424 174 1396 Fragments arranged by size: 4424 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5821 6008 1700 Size: 110 4011 187 2869 Fragments arranged by size: 4011 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3893 96 Size: 3797 3380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 3335 5237 6249 3335 Size: 1902 1012 4263 Fragments arranged by size: 4263 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3272 3666 3810 794 Size: 2478 394 144 4161 Fragments arranged by size: 4161 2478 394 144 BstBI TT'CG_AA Cuts at: 5981 5981 Size: 7177 BstEII G'GTnAC_C Cuts at: 1184 4402 1184 Size: 3218 3959 BstYI r'GATC_y Cuts at: 1972 2179 2659 3709 4384 4507 4874 5259 5276 Size: 207 480 1050 675 123 367 385 17 Cuts at: 5276 6728 6814 6826 7172 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1050 675 480 385 367 346 207 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3085 513 Size: 552 2020 4605 Fragments arranged by size: 4605 2020 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2687 487 Size: 683 56 1461 4977 Fragments arranged by size: 4977 1461 683 56 BtsI GCAGTG_nn' Cuts at: 3553 3667 4693 5487 5507 3553 Size: 114 1026 794 20 5223 Fragments arranged by size: 5223 1026 794 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 2242 2565 2821 4432 4481 2242 Size: 323 256 1611 49 4938 Fragments arranged by size: 4938 1611 323 256 49 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4400 6184 809 Size: 507 3084 1784 1802 Fragments arranged by size: 3084 1802 1784 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3829 4624 5513 Size: 54 1425 50 275 18 1999 795 889 Cuts at: 5513 7142 8 Size: 1629 43 Fragments arranged by size: 1999 1629 1425 889 795 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4624 1537 Size: 275 2812 4090 Fragments arranged by size: 4090 2812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2574 2616 2641 2727 3459 5107 1353 Size: 1221 42 25 86 732 1648 3423 Fragments arranged by size: 3423 1648 1221 732 86 42 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5764 6138 6284 387 Size: 1064 138 4175 374 146 1280 Fragments arranged by size: 4175 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2640 3660 3708 3788 4194 5240 Size: 393 900 108 1020 48 80 406 1046 Cuts at: 5240 5837 6624 6892 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1046 1020 900 787 597 406 393 268 108 80 48 EcoNI CCTnn'n_nnAGG Cuts at: 3191 3191 Size: 7177 EcoO109I rG'GnC_Cy Cuts at: 3820 4342 3820 Size: 522 6655 EcoRI G'AATT_C Cuts at: 4486 4864 4486 Size: 378 6799 EcoRV GAT'ATC Cuts at: 2518 2518 Size: 7177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4363 4363 Size: 7177 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 2057 4250 6324 1678 Size: 379 2193 2074 2531 Fragments arranged by size: 2531 2193 2074 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2123 2155 2975 3007 3316 Size: 32 135 32 551 32 820 32 309 Cuts at: 3316 3348 4154 4186 5818 5850 5892 5924 1373 Size: 32 806 32 1632 32 42 32 2626 Fragments arranged by size: 2626 1632 820 806 551 309 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4456 4521 4767 669 Size: 988 281 2518 65 246 3079 Fragments arranged by size: 3079 2518 988 281 246 65 HindIII A'AGCT_T Cuts at: 748 2105 748 Size: 1357 5820 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KasI G'GCGC_C Cuts at: 2053 4246 2053 Size: 2193 4984 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6291 6475 1305 Size: 4986 184 2007 Fragments arranged by size: 4986 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5355 Fragments arranged by size: 5355 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3090 3320 5135 5494 5653 518 Size: 648 1924 230 1815 359 159 2042 Fragments arranged by size: 2042 1924 1815 648 359 230 159 MspA1I CmG'CkG Cuts at: 1606 3766 3834 3979 4367 4593 5270 6418 6663 Size: 2160 68 145 388 226 677 1148 245 Cuts at: 6663 1606 Size: 2120 Fragments arranged by size: 2160 2120 1148 677 388 245 226 145 68 NarI GG'CG_CC Cuts at: 2054 4247 2054 Size: 2193 4984 NcoI C'CATG_G Cuts at: 513 1065 3085 513 Size: 552 2020 4605 Fragments arranged by size: 4605 2020 552 NdeI CA'TA_TG Cuts at: 387 4317 387 Size: 3930 3247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NsiI A_TGCA'T Cuts at: 3740 3740 Size: 7177 NspI r_CATG'y Cuts at: 1132 1229 2201 3783 4535 6080 1132 Size: 97 972 1582 752 1545 2229 Fragments arranged by size: 2229 1582 1545 972 752 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7177 PciI A'CATG_T Cuts at: 2197 6076 2197 Size: 3879 3298 PflMI CCAn_nnn'nTGG Cuts at: 1108 4084 4279 1108 Size: 2976 195 4006 Fragments arranged by size: 4006 2976 195 PfoI T'CCnGG_A Cuts at: 3676 4290 3676 Size: 614 6563 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PmlI CAC'GTG Cuts at: 2359 2676 2359 Size: 317 6860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5246 5278 6759 6791 5246 Size: 32 1481 32 5632 Fragments arranged by size: 5632 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3820 4342 3820 Size: 522 6655 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 2414 2505 4747 2414 Size: 91 2242 4844 Fragments arranged by size: 4844 2242 91 PspXI vC'TCGA_Gb Cuts at: 3445 3445 Size: 7177 PstI C_TGCA'G Cuts at: 830 3365 3818 4224 4440 4529 830 Size: 2535 453 406 216 89 3478 Fragments arranged by size: 3478 2535 453 406 216 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 3766 3979 4367 3766 Size: 213 388 6576 Fragments arranged by size: 6576 388 213 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SapI GCTCTTCn'nnn_ Cuts at: 2616 2641 2616 Size: 25 7152 SbfI CC_TGCA'GG Cuts at: 4529 4529 Size: 7177 ScaI AGT'ACT Cuts at: 2263 5425 7137 2263 Size: 3162 1712 2303 Fragments arranged by size: 3162 2303 1712 SexAI A'CCwGG_T Cuts at: 2881 3204 2881 Size: 323 6854 SfcI C'TryA_G Cuts at: 826 1045 2183 2710 3361 3814 4220 4436 4525 Size: 219 1138 527 651 453 406 216 89 Cuts at: 4525 5660 6341 6532 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 651 527 453 406 219 216 191 89 SfoI GGC'GCC Cuts at: 2055 4248 2055 Size: 2193 4984 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 2076 4504 1872 Size: 204 2428 4545 Fragments arranged by size: 4545 2428 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 2858 3445 5281 6182 6444 Size: 197 926 156 759 587 1836 901 262 Cuts at: 6444 820 Size: 1553 Fragments arranged by size: 1836 1553 926 901 759 587 262 197 156 SnaBI TAC'GTA Cuts at: 493 4968 493 Size: 4475 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 4535 1132 Size: 3403 3774 SspI AAT'ATT Cuts at: 5 52 5101 5 Size: 47 5049 2081 Fragments arranged by size: 5049 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7177 StyI C'CwwG_G Cuts at: 513 1065 3085 4616 513 Size: 552 2020 1531 3074 Fragments arranged by size: 3074 2020 1531 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5204 5221 5374 5559 6004 325 Size: 1255 3624 17 153 185 445 1498 Fragments arranged by size: 3624 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2261 2322 2738 Size: 274 80 33 51 1443 284 61 416 Cuts at: 2738 3893 3937 5423 7135 96 Size: 1155 44 1486 1712 138 Fragments arranged by size: 1712 1486 1443 1155 416 284 274 138 80 61 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4381 4413 7137 7169 4381 Size: 32 2724 32 4389 Fragments arranged by size: 4389 2724 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XhoI C'TCGA_G Cuts at: 1943 2099 3445 1943 Size: 156 1346 5675 Fragments arranged by size: 5675 1346 156 XmaI C'CCGG_G Cuts at: 1870 2074 4502 1870 Size: 204 2428 4545 Fragments arranged by size: 4545 2428 204 XmnI GAAnn'nnTTC Cuts at: 5306 5306 Size: 7177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI