(Linear) (Six-base) MAPSORT of: KIEE1898.seq Check: 8025 from: 1 to: 2472 KIEE1898.orf With 182 enzymes: SgfI * August 13, 2015 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1507 2472 Size: 1507 965 AccI GT'mk_AC Cuts at: 0 599 2472 Size: 599 1873 AclI AA'CG_TT Cuts at: 0 264 2472 Size: 264 2208 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 527 635 1703 2472 Size: 527 108 1068 769 Fragments arranged by size: 1068 769 527 108 AflIII A'CryG_T Cuts at: 0 192 351 668 670 2472 Size: 192 159 317 2 1802 Fragments arranged by size: 1802 317 192 159 2 AlwNI CAG_nnn'CTG Cuts at: 0 1514 1761 2472 Size: 1514 247 711 Fragments arranged by size: 1514 711 247 ApoI r'AATT_y Cuts at: 0 276 2472 Size: 276 2196 AseI AT'TA_AT Cuts at: 0 1613 2472 Size: 1613 859 AvaI C'yCGr_G Cuts at: 0 69 94 1440 2472 Size: 69 25 1346 1032 Fragments arranged by size: 1346 1032 69 25 BanI G'GyrC_C Cuts at: 0 48 2241 2472 Size: 48 2193 231 Fragments arranged by size: 2193 231 48 BbeI G_GCGC'C Cuts at: 0 52 2245 2472 Size: 52 2193 227 Fragments arranged by size: 2193 227 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 2297 2472 Size: 2297 175 BbvCI CC'TCA_GC Cuts at: 0 977 2472 Size: 977 1495 BciVI GTATCCnnnnn_n' Cuts at: 0 1316 2472 Size: 1316 1156 BfrBI ATG'CAT Cuts at: 0 1733 2472 Size: 1733 739 BglII A'GATC_T Cuts at: 0 174 2472 Size: 174 2298 BlpI GC'TnA_GC Cuts at: 0 1975 2146 2472 Size: 1975 171 326 Fragments arranged by size: 1975 326 171 Bme1580I G_kGCm'C Cuts at: 0 1272 1666 1838 2472 Size: 1272 394 172 634 Fragments arranged by size: 1272 634 394 172 BmrI ACTGGGnnnn_n' Cuts at: 0 1108 2123 2472 Size: 1108 1015 349 Fragments arranged by size: 1108 1015 349 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 26 387 2472 Size: 26 361 2085 Fragments arranged by size: 2085 361 26 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1655 1783 2189 2472 Size: 1655 128 406 283 Fragments arranged by size: 1655 406 283 128 Bpu10I CC'TnA_GC Cuts at: 0 977 2472 Size: 977 1495 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 838 2472 Size: 838 1634 BsaAI yAC'GTr Cuts at: 0 354 671 2472 Size: 354 317 1801 Fragments arranged by size: 1801 354 317 BsaHI Gr'CG_yC Cuts at: 0 49 2242 2472 Size: 49 2193 230 Fragments arranged by size: 2193 230 49 BsaWI w'CCGG_w Cuts at: 0 60 1248 2394 2472 Size: 60 1188 1146 78 Fragments arranged by size: 1188 1146 78 60 BseYI C'CCAG_C Cuts at: 0 2296 2472 Size: 2296 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1500 1560 1626 2472 Size: 1500 60 66 846 Fragments arranged by size: 1500 846 66 60 BsiHKAI G_wGCw'C Cuts at: 0 1159 2472 Size: 1159 1313 BsmI GAATG_Cn' Cuts at: 0 156 848 2063 2472 Size: 156 692 1215 409 Fragments arranged by size: 1215 692 409 156 Bsp1286I G_dGCh'C Cuts at: 0 1159 1272 1666 1838 2472 Size: 1159 113 394 172 634 Fragments arranged by size: 1159 634 394 172 113 BspEI T'CCGG_A Cuts at: 0 60 1248 2472 Size: 60 1188 1224 Fragments arranged by size: 1224 1188 60 BspHI T'CATG_A Cuts at: 0 1212 2472 Size: 1212 1260 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 799 1507 2472 Size: 799 708 965 Fragments arranged by size: 965 799 708 BsrGI T'GTAC_A Cuts at: 0 1888 2472 Size: 1888 584 BssSI C'ACGA_G Cuts at: 0 1330 2472 Size: 1330 1142 BstAPI GCAn_nnn'nTGC Cuts at: 0 1267 1661 1805 2472 Size: 1267 394 144 667 Fragments arranged by size: 1267 667 394 144 BstEII G'GTnAC_C Cuts at: 0 2397 2472 Size: 2397 75 BstYI r'GATC_y Cuts at: 0 174 654 1704 2379 2472 Size: 174 480 1050 675 93 Fragments arranged by size: 1050 675 480 174 93 BtgI C'CryG_G Cuts at: 0 1080 2472 Size: 1080 1392 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 682 2472 Size: 682 1790 BtsI GCAGTG_nn' Cuts at: 0 1548 1662 2472 Size: 1548 114 810 Fragments arranged by size: 1548 810 114 DraI TTT'AAA Cuts at: 0 237 560 816 2427 2472 Size: 237 323 256 1611 45 Fragments arranged by size: 1611 323 256 237 45 DrdI GACnn_nn'nnGTC Cuts at: 0 2395 2472 Size: 2395 77 EaeI y'GGCC_r Cuts at: 0 1824 2472 Size: 1824 648 EarI CTCTTCn'nnn_ Cuts at: 0 569 611 636 722 1454 2472 Size: 569 42 25 86 732 1018 Fragments arranged by size: 1018 732 569 86 42 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 527 635 1655 1703 1783 2189 2472 Size: 527 108 1020 48 80 406 283 Fragments arranged by size: 1020 527 406 283 108 80 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 1186 2472 Size: 1186 1286 EcoO109I rG'GnC_Cy Cuts at: 0 1815 2337 2472 Size: 1815 522 135 Fragments arranged by size: 1815 522 135 EcoRV GAT'ATC Cuts at: 0 513 2472 Size: 513 1959 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2358 2472 Size: 2358 114 HaeII r_GCGC'y Cuts at: 0 52 2245 2472 Size: 52 2193 227 Fragments arranged by size: 2193 227 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 118 150 970 1002 1311 1343 2149 2181 Size: 118 32 820 32 309 32 806 32 Cuts at: 2181 2472 Size: 291 Fragments arranged by size: 820 806 309 291 118 32 32 32 32 HincII GTy'rAC Cuts at: 0 2451 2472 Size: 2451 21 HindIII A'AGCT_T Cuts at: 0 100 2472 Size: 100 2372 KasI G'GCGC_C Cuts at: 0 48 2241 2472 Size: 48 2193 231 Fragments arranged by size: 2193 231 48 MslI CAynn'nnrTG Cuts at: 0 1085 1315 2472 Size: 1085 230 1157 Fragments arranged by size: 1157 1085 230 MspA1I CmG'CkG Cuts at: 0 1761 1829 1974 2362 2472 Size: 1761 68 145 388 110 Fragments arranged by size: 1761 388 145 110 68 NarI GG'CG_CC Cuts at: 0 49 2242 2472 Size: 49 2193 230 Fragments arranged by size: 2193 230 49 NcoI C'CATG_G Cuts at: 0 1080 2472 Size: 1080 1392 NdeI CA'TA_TG Cuts at: 0 2312 2472 Size: 2312 160 NsiI A_TGCA'T Cuts at: 0 1735 2472 Size: 1735 737 NspI r_CATG'y Cuts at: 0 196 1778 2472 Size: 196 1582 694 Fragments arranged by size: 1582 694 196 PciI A'CATG_T Cuts at: 0 192 2472 Size: 192 2280 PflMI CCAn_nnn'nTGG Cuts at: 0 2079 2274 2472 Size: 2079 195 198 Fragments arranged by size: 2079 198 195 PfoI T'CCnGG_A Cuts at: 0 1671 2285 2472 Size: 1671 614 187 Fragments arranged by size: 1671 614 187 PmlI CAC'GTG Cuts at: 0 354 671 2472 Size: 354 317 1801 Fragments arranged by size: 1801 354 317 PpuMI rG'GwC_Cy Cuts at: 0 1815 2337 2472 Size: 1815 522 135 Fragments arranged by size: 1815 522 135 PsiI TTA'TAA Cuts at: 0 409 500 2472 Size: 409 91 1972 Fragments arranged by size: 1972 409 91 PspXI vC'TCGA_Gb Cuts at: 0 1440 2472 Size: 1440 1032 PstI C_TGCA'G Cuts at: 0 1360 1813 2219 2435 2472 Size: 1360 453 406 216 37 Fragments arranged by size: 1360 453 406 216 37 PvuII CAG'CTG Cuts at: 0 1761 1974 2362 2472 Size: 1761 213 388 110 Fragments arranged by size: 1761 388 213 110 SapI GCTCTTCn'nnn_ Cuts at: 0 611 636 2472 Size: 611 25 1836 Fragments arranged by size: 1836 611 25 ScaI AGT'ACT Cuts at: 0 258 2472 Size: 258 2214 SexAI A'CCwGG_T Cuts at: 0 876 1199 2472 Size: 876 323 1273 Fragments arranged by size: 1273 876 323 SfcI C'TryA_G Cuts at: 0 178 705 1356 1809 2215 2431 2472 Size: 178 527 651 453 406 216 41 Fragments arranged by size: 651 527 453 406 216 178 41 SfoI GGC'GCC Cuts at: 0 50 2243 2472 Size: 50 2193 229 Fragments arranged by size: 2193 229 50 SmaI CCC'GGG Cuts at: 0 71 2472 Size: 71 2401 SmlI C'TyrA_G Cuts at: 0 94 853 1440 2472 Size: 94 759 587 1032 Fragments arranged by size: 1032 759 587 94 StyI C'CwwG_G Cuts at: 0 1080 2472 Size: 1080 1392 TatI w'GTAC_w Cuts at: 0 256 317 733 1888 1932 2472 Size: 256 61 416 1155 44 540 Fragments arranged by size: 1155 540 416 256 61 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2376 2408 2472 Size: 2376 32 64 Fragments arranged by size: 2376 64 32 XhoI C'TCGA_G Cuts at: 0 94 1440 2472 Size: 94 1346 1032 Fragments arranged by size: 1346 1032 94 XmaI C'CCGG_G Cuts at: 0 69 2472 Size: 69 2403 Enzymes that do cut: AarI AccI AclI AcuI AflIII AlwNI ApoI AseI AvaI BanI BbeI BbsI BbvCI BciVI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrGI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MslI MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI PfoI PmlI PpuMI PsiI PspXI PstI PvuII SapI ScaI SexAI SfcI SfoI SmaI SmlI StyI TatI TstI XhoI XmaI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII BcgI BclI BglI BmgBI BmtI BsaI BsaBI BsaXI BseRI BsiEI BsiWI BsmBI BsrBI BsrDI BsrFI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraIII EagI EciI EcoICRI EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI NaeI NgoMIV NheI NotI NruI PacI PasI PmeI PpiI PshAI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XmnI ZraI