(Circular) (Six-base) MAPSORT of: pFN21AE6605.seq Check: 2619 from: 1 to: 5518 pFN21AE6605 5518 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3581 4965 5233 1632 Size: 1949 1384 268 1917 Fragments arranged by size: 1949 1917 1384 268 AfeI AGC'GCT Cuts at: 2489 2489 Size: 5518 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2372 1184 Size: 1188 4330 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 2319 Size: 32 5486 AlwNI CAG_nnn'CTG Cuts at: 3261 4833 3261 Size: 1572 3946 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2746 2843 1870 Size: 73 803 97 4545 Fragments arranged by size: 4545 803 97 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2639 2839 4294 618 Size: 325 217 627 375 477 200 1455 1842 Fragments arranged by size: 1842 1455 627 477 375 325 217 200 BanII G_rGCy'C Cuts at: 1391 2412 2703 2837 1391 Size: 1021 291 134 4072 Fragments arranged by size: 4072 1021 291 134 BbeI G_GCGC'C Cuts at: 2166 2643 2166 Size: 477 5041 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2599 928 Size: 971 700 3847 Fragments arranged by size: 3847 971 700 BbvCI CC'TCA_GC Cuts at: 2079 2079 Size: 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2350 2384 3709 3743 1903 Size: 34 413 34 1325 34 3678 Fragments arranged by size: 3678 1325 413 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 2597 4129 Size: 107 122 71 631 605 854 71 1532 Cuts at: 4129 136 Size: 1525 Fragments arranged by size: 1532 1525 854 631 605 122 107 71 71 BglII A'GATC_T Cuts at: 2460 5513 2460 Size: 3053 2465 BlpI GC'TnA_GC Cuts at: 2534 2935 2534 Size: 401 5117 Bme1580I G_kGCm'C Cuts at: 1790 2531 3579 4735 1790 Size: 741 1048 1156 2573 Fragments arranged by size: 2573 1156 1048 741 BmgBI CAC'GTC Cuts at: 1318 2764 1318 Size: 1446 4072 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2648 4178 1239 Size: 1409 1530 2579 Fragments arranged by size: 2579 1530 1409 Bpu10I CC'TnA_GC Cuts at: 2079 2079 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 3643 4508 4806 2396 Size: 1247 865 298 3108 Fragments arranged by size: 3108 1247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2395 4181 882 Size: 617 896 1786 2219 Fragments arranged by size: 2219 1786 896 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2163 2499 2640 Size: 53 83 186 936 564 66 336 141 Cuts at: 2640 3325 3707 4342 275 Size: 685 382 635 1451 Fragments arranged by size: 1451 936 685 635 564 382 336 186 141 83 66 53 BsaWI w'CCGG_w Cuts at: 2294 3951 4349 4623 4770 5181 2294 Size: 1657 398 274 147 411 2631 Fragments arranged by size: 2631 1657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2319 2349 1178 Size: 30 165 30 916 30 4347 Fragments arranged by size: 4347 916 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 2735 1186 Size: 1309 240 3969 Fragments arranged by size: 3969 1309 240 BseYI C'CCAG_C Cuts at: 2502 4721 5217 2502 Size: 2219 496 2803 Fragments arranged by size: 2803 2219 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2368 1163 Size: 573 393 239 4313 Fragments arranged by size: 4313 573 393 239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 3579 3664 4735 2412 Size: 1167 85 1071 3195 Fragments arranged by size: 3195 1167 1071 85 BsmI GAATG_Cn' Cuts at: 2431 3029 3122 2431 Size: 598 93 4827 Fragments arranged by size: 4827 598 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2531 2703 2837 3579 3664 4735 Size: 399 622 119 172 134 742 85 1071 Cuts at: 4735 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 622 399 172 134 119 85 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 2873 844 Size: 1631 398 3489 Fragments arranged by size: 3489 1631 398 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2435 2591 4162 4349 1700 Size: 110 273 352 156 1571 187 2869 Fragments arranged by size: 2869 1571 352 273 187 156 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2112 2460 2848 3215 3600 3617 5069 5155 Size: 140 348 388 367 385 17 1452 86 Cuts at: 5155 5167 5513 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 388 385 367 348 346 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 2521 513 Size: 552 1128 328 3510 Fragments arranged by size: 3510 1128 552 328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2106 487 Size: 683 56 880 3899 Fragments arranged by size: 3899 880 683 56 BtsI GCAGTG_nn' Cuts at: 2057 2242 3034 3828 3848 2057 Size: 185 792 794 20 3727 Fragments arranged by size: 3727 794 792 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2313 2387 4525 809 Size: 507 997 74 2138 1802 Fragments arranged by size: 2138 1802 997 507 74 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2965 3854 Size: 54 1425 50 275 18 741 394 889 Cuts at: 3854 5483 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 741 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 3448 1353 Size: 823 1272 3423 Fragments arranged by size: 3423 1272 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 2410 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2648 3581 4178 4965 5233 1239 Size: 393 1016 933 597 787 268 1524 Fragments arranged by size: 1524 1016 933 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2630 2630 Size: 5518 EcoO109I rG'GnC_Cy Cuts at: 2306 2306 Size: 5518 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 2360 4024 2360 Size: 1664 3854 HaeII r_GCGC'y Cuts at: 1678 2166 2491 2643 4665 1678 Size: 488 325 152 2022 2531 Fragments arranged by size: 2531 2022 488 325 152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2087 2119 4159 Size: 32 135 32 427 32 56 32 2040 Cuts at: 4159 4191 4233 4265 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2040 427 135 56 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KasI G'GCGC_C Cuts at: 2162 2639 2162 Size: 477 5041 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2344 2780 3476 3835 3994 518 Size: 648 1178 436 696 359 159 2042 Fragments arranged by size: 2042 1178 696 648 436 359 159 MspA1I CmG'CkG Cuts at: 1606 2356 2398 2506 2523 2694 2934 3611 4759 Size: 750 42 108 17 171 240 677 1148 Cuts at: 4759 5004 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 750 677 245 240 171 108 42 17 NaeI GCC'GGC Cuts at: 2085 2593 2085 Size: 508 5010 NarI GG'CG_CC Cuts at: 2163 2640 2163 Size: 477 5041 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4966 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NgoMIV G'CCGG_C Cuts at: 2083 2591 2083 Size: 508 5010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2542 2876 4421 1132 Size: 97 1313 334 1545 2229 Fragments arranged by size: 2229 1545 1313 334 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2319 2351 2399 2431 3587 3619 5100 5132 2319 Size: 32 48 32 1156 32 1481 32 2705 Fragments arranged by size: 2705 1481 1156 48 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2306 2306 Size: 5518 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PstI C_TGCA'G Cuts at: 830 2870 830 Size: 2040 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2356 2398 2506 2356 Size: 42 108 5368 Fragments arranged by size: 5368 108 42 SacI G_AGCT'C Cuts at: 2412 2412 Size: 5518 SacII CC_GC'GG Cuts at: 2524 2524 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SexAI A'CCwGG_T Cuts at: 2385 2385 Size: 5518 SfcI C'TryA_G Cuts at: 826 1045 2866 4001 4682 4873 826 Size: 219 1821 1135 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2164 2641 2164 Size: 477 5041 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 2435 1700 Size: 735 4783 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3622 4523 4785 820 Size: 197 926 468 1211 901 262 1553 Fragments arranged by size: 1553 1211 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2542 2876 1132 Size: 1410 334 3774 Fragments arranged by size: 3774 1410 334 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 2334 1517 Size: 817 4701 StyI C'CwwG_G Cuts at: 513 1065 2544 2957 513 Size: 552 1479 413 3074 Fragments arranged by size: 3074 1479 552 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 2784 3764 Size: 274 80 33 51 1443 672 135 980 Cuts at: 3764 5476 96 Size: 1712 138 Fragments arranged by size: 1712 1443 980 672 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 2566 2374 Size: 192 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI