Restriction map for
pFN21AE9428
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BlnI BsaBI BsaHI BseAI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI DraI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 142 GTCTAC GT^[AC][GT]AC
AcsI 516 GAATTT [GA]^AATT[CT]
AgeI 461 ACCGGT A^CCGGT
AocI 1072 CCTCAGG CC^T[ACGT]AGG
ApoI 516 GAATTT [GA]^AATT[CT]
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
BalI 584 TGGCCA TGG^CCA
BamHI 1173 GGATCC G^GATCC
BanII 687 GGGCTC G[GA]GC[CT]^C
BciVI 868 GGATAC GTATCC(6/5)
BglII 1006 AGATCT A^GATCT
BsaI 49 GAGACC GGTCTC(1/5)
BsaWI 461 ACCGGT [AT]^CCGG[AT]
BseRI 690 CTCCTC GAGGAG(10/8)
BsgI 184 GTGCAG GTGCAG(16/14)
BspMI 411 GCAGGT ACCTGC(4/8)
BsrDI 1065 CATTGC GCAATG(2/0)
BsrFI 461 ACCGGT [GA]^CCGG[CT]
BsrI 815 ACTGG ACTGG(1/-1)
BstF5I 768 CATCC GGATG(2/0)
Bsu36I 1072 CCTCAGG CC^T[ACGT]AGG
Cfr10I 461 ACCGGT [GA]^CCGG[CT]
CpoI 274 CGGACCG CG^G[AT]CCG
Eco81I 1072 CCTCAGG CC^T[ACGT]AGG
FokI 768 CATCC GGATG(9/13)
HaeII 31 AGCGCC [GA]GCGC^[CT]
HindIII 124 AAGCTT A^AGCTT
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MspA1I 853 CAGCTG C[AC]G^C[GT]G
NcoI 990 CCATGG C^CATGG
NlaIII 991 CATG CATG^
PflMI 1186 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 461 ACCGGT A^CCGGT
PshBI 589 ATTAAT AT^TAAT
PstI 370 CTGCAG CTGCA^G
PvuII 853 CAGCTG CAG^CTG
RsrII 274 CGGACCG CG^G[AT]CCG
SfaNI 862 GATGC GCATC(5/9)
SfcI 370 CTGCAG C^T[GA][CT]AG
SnaBI 871 TACGTA TAC^GTA
SspI 437 AATATT AAT^ATT
TaqI 1029 TCGA T^CGA
TspRI 1041 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1029 TCGA T^CGA
Van91I 1186 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 402 GTAC GT^AC
819 GTAC
AlwI 1174 GATCC GGATC(4/5)
1173 GGATC
AvaI 7 CCCGAG C^[CT]CG[GA]G
746 CTCGGG
BbsI 238 GTCTTC GAAGAC(2/6)
337 GTCTTC
BlpI 222 GCTCAGC GC^T[ACGT]AGC
324 GCTCAGC
BmyI 109 GTGCCC G[AGT]GC[ACT]^C
687 GGGCTC
BpmI 443 CTCCAG CTGGAG(16/14)
1097 CTCCAG
Bpu1102I 222 GCTCAGC GC^T[ACGT]AGC
324 GCTCAGC
BpuAI 238 GTCTTC GAAGAC(2/6)
337 GTCTTC
BsiEI 19 CGGCCG CG[GA][CT]^CG
104 CGGCCG
BsmBI 570 CGTCTC CGTCTC(1/5)
618 CGTCTC
BsoBI 7 CCCGAG C^[CT]CG[GA]G
746 CTCGGG
Bsp1286I 109 GTGCCC G[AGT]GC[ACT]^C
687 GGGCTC
BstYI 1006 AGATCT [GA]^GATC[CT]
1173 GGATCC
CelII 222 GCTCAGC GC^T[ACGT]AGC
324 GCTCAGC
DpnII 1007 GATC ^GATC
1174 GATC
DraII 62 GGGACCC [GA]G^G[ACGT]CC[CT]
959 AGGACCT
DsaI 58 CCGTGG C^C[GA][CT]GG
990 CCATGG
EagI 19 CGGCCG C^GGCCG
104 CGGCCG
EclXI 19 CGGCCG C^GGCCG
104 CGGCCG
Eco52I 19 CGGCCG C^GGCCG
104 CGGCCG
EcoO109I 62 GGGACCC [GA]G^G[ACGT]CC[CT]
959 AGGACCT
FauI 54 CCCGC CCCGC(4/6)
71 CCCGC
MboI 1007 GATC ^GATC
1174 GATC
MflI 1006 AGATCT [GA]^GATC[CT]
1173 GGATCC
MseI 520 TTAA T^TAA
590 TTAA
NdeII 1007 GATC ^GATC
1174 GATC
PpuMI 62 GGGACCC [GA]G^G[AT]CC[CT]
959 AGGACCT
RsaI 402 GTAC GT^AC
819 GTAC
SapI 165 GCTCTTC GCTCTTC(1/4)
600 GCTCTTC
Sau3AI 1007 GATC ^GATC
1174 GATC
TfiI 849 GAATC G^A[AT]TC
995 GATTC
Tru9I 520 TTAA T^TAA
590 TTAA
Tsp45I 706 GTGAC ^GT[GC]AC
721 GTCAC
XcmI 665 CCAACATTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
968 CCAACCCTGGAATGG
XhoII 1006 AGATCT [GA]^GATC[CT]
1173 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EarI EcoRII EcoT14I Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NlaIV
PleI Sau96I ScrFI StyI TseI
Tsp509I