(Circular) (Six-base) MAPSORT of: pFN21AE9428.seq Check: 527 from: 1 to: 5902 pFN21AE9428 5902 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2407 2407 Size: 5902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 3245 1937 Size: 211 1097 4594 Fragments arranged by size: 4594 1097 211 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3071 3965 5349 5617 1632 Size: 1439 894 1384 268 1917 Fragments arranged by size: 1917 1439 1384 894 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 2466 4733 2466 Size: 2267 3635 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 2417 1166 Size: 1251 4651 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2521 3211 3437 3589 5698 1493 Size: 1028 690 226 152 2109 1697 Fragments arranged by size: 2109 1697 1028 690 226 152 AseI AT'TA_AT Cuts at: 160 1032 2595 4456 160 Size: 872 1563 1861 1606 Fragments arranged by size: 1861 1606 1563 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2751 3227 1870 Size: 73 69 739 476 4545 Fragments arranged by size: 4545 739 476 73 69 BamHI G'GATC_C Cuts at: 3178 3232 3599 3178 Size: 54 367 5481 Fragments arranged by size: 5481 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 2696 3221 1391 Size: 1305 525 4072 Fragments arranged by size: 4072 1305 525 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2236 2335 928 Size: 971 337 99 4495 Fragments arranged by size: 4495 971 337 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2867 3794 5004 2867 Size: 927 1210 3765 Fragments arranged by size: 3765 1210 927 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3011 5897 3011 Size: 2886 3016 BlpI GC'TnA_GC Cuts at: 2228 2330 3319 2228 Size: 102 989 4811 Fragments arranged by size: 4811 989 102 Bme1580I G_kGCm'C Cuts at: 1790 2118 3963 5119 1790 Size: 328 1845 1156 2573 Fragments arranged by size: 2573 1845 1156 328 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 3087 4562 1239 Size: 1194 654 1475 2579 Fragments arranged by size: 2579 1475 1194 654 Bpu10I CC'TnA_GC Cuts at: 2665 2665 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 4565 882 Size: 617 549 2517 2219 Fragments arranged by size: 2517 2219 617 549 BsaAI yAC'GTr Cuts at: 493 1204 2125 2188 2878 3693 493 Size: 711 921 63 690 815 2702 Fragments arranged by size: 2702 921 815 711 690 63 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2466 4335 4733 5007 5154 5565 2466 Size: 1869 398 274 147 411 2803 Fragments arranged by size: 2803 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2471 2501 1178 Size: 30 165 30 1068 30 4579 Fragments arranged by size: 4579 1068 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2686 1186 Size: 1500 4402 BseYI C'CCAG_C Cuts at: 2071 2860 2990 5105 5601 2071 Size: 789 130 2115 496 2372 Fragments arranged by size: 2372 2115 789 496 130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 1163 Size: 573 474 4855 Fragments arranged by size: 4855 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2027 2112 3352 4113 5141 1540 Size: 275 186 26 85 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 85 26 BsiHKAI G_wGCw'C Cuts at: 3963 4048 5119 3963 Size: 85 1071 4746 Fragments arranged by size: 4746 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 BsmBI CGTCTCn'nnnn_ Cuts at: 2581 2629 2581 Size: 48 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2696 3221 3963 4048 5119 1391 Size: 399 328 578 525 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 525 399 328 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 3257 844 Size: 1563 850 3489 Fragments arranged by size: 3489 1563 850 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4397 4571 65 Size: 1183 1821 1328 174 1396 Fragments arranged by size: 1821 1396 1328 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2466 4546 4733 1700 Size: 110 656 2080 187 2869 Fragments arranged by size: 2869 2080 656 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 3011 3178 3232 3599 3984 4001 5453 5539 Size: 1039 167 54 367 385 17 1452 86 Cuts at: 5539 5551 5897 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1039 385 367 346 167 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 3078 3078 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2063 2995 513 Size: 552 998 932 3420 Fragments arranged by size: 3420 998 932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 3418 4212 4232 3418 Size: 794 20 5088 Fragments arranged by size: 5088 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 2109 2319 Size: 54 1425 50 275 18 194 85 210 Cuts at: 2319 2589 3349 4238 5867 8 Size: 270 760 889 1629 43 Fragments arranged by size: 1629 1425 889 760 275 270 210 194 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2024 2109 3349 1537 Size: 275 212 85 1240 4090 Fragments arranged by size: 4090 1240 275 212 85 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2267 2294 2498 2612 3832 1353 Size: 824 90 27 204 114 1220 3423 Fragments arranged by size: 3423 1220 824 204 114 90 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 3071 3087 3965 4562 5349 5617 Size: 393 801 638 16 878 597 787 268 Cuts at: 5617 1239 Size: 1524 Fragments arranged by size: 1524 878 801 787 638 597 393 268 16 EcoO109I rG'GnC_Cy Cuts at: 2068 2965 2068 Size: 897 5005 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2680 2680 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2040 5049 1678 Size: 362 3009 2531 Fragments arranged by size: 3009 2531 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373 Size: 32 135 32 2971 32 42 32 2626 Fragments arranged by size: 2971 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 2129 748 Size: 1381 4521 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3321 Fragments arranged by size: 3321 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2396 2417 3860 4219 4378 518 Size: 648 1020 210 21 1443 359 159 2042 Fragments arranged by size: 2042 1443 1020 648 359 210 159 21 MspA1I CmG'CkG Cuts at: 1606 2860 3318 3995 5143 5388 1606 Size: 1254 458 677 1148 245 2120 Fragments arranged by size: 2120 1254 1148 677 458 245 NcoI C'CATG_G Cuts at: 513 1065 2995 513 Size: 552 1930 3420 Fragments arranged by size: 3420 1930 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 3260 4805 1132 Size: 97 2031 1545 2229 Fragments arranged by size: 2229 2031 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 3197 1108 Size: 2089 3813 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2965 2068 Size: 897 5005 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 2379 3254 830 Size: 1549 875 3478 Fragments arranged by size: 3478 1549 875 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2860 2860 Size: 5902 RsrII CG'GwC_CG Cuts at: 2280 2280 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SanDI GG'GwC_CC Cuts at: 2068 2068 Size: 5902 SapI GCTCTTCn'nnn_ Cuts at: 2177 2612 2177 Size: 435 5467 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2375 3250 4385 5066 5257 826 Size: 219 1330 875 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 875 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2878 3693 493 Size: 2385 815 2702 Fragments arranged by size: 2702 2385 815 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2444 3826 5 Size: 47 2392 1382 2081 Fragments arranged by size: 2392 2081 1382 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2688 2995 3196 3341 513 Size: 552 1623 307 201 145 3074 Fragments arranged by size: 3074 1623 552 307 201 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3929 3946 4099 4284 4729 325 Size: 1255 2349 17 153 185 445 1498 Fragments arranged by size: 2349 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 4148 5860 Size: 274 80 33 51 1443 429 1742 1712 Cuts at: 5860 96 Size: 138 Fragments arranged by size: 1742 1712 1443 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2677 2980 2677 Size: 303 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI