################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BbeI BbrPI BciVI BfrI BglI BlnI BsaAI BsaHI BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 998 GTCTAC GT^[AC][GT]AC AhdI 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1277 GTGCAC G^TGCAC ApaLI 1277 GTGCAC G^TGCAC AspEI 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1277 GTGCAC G[AT]GC[AT]^C BalI 1086 TGGCCA TGG^CCA BanII 1398 GGGCTC G[GA]GC[CT]^C BbvCI 655 GCTGAGG CCTCAGC(-5/-2) BbvI 663 GCTGC GCAGC(8/12) BcgI 1499 GCAGAGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 982 CTAG C^TAG BglII 771 AGATCT A^GATCT BsaBI 796 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 410 GTGCAG GTGCAG(16/14) BsiHKAI 1277 GTGCAC G[AT]GC[AT]^C BsmBI 1054 GAGACG CGTCTC(1/5) BspMI 824 ACCTGC ACCTGC(4/8) BsrDI 519 CATTGC GCAATG(2/0) CfoI 1060 GCGC GCG^C DraI 175 TTTAAA TTT^AAA DraII 99 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 92 CCGTGG C^C[GA][CT]GG EaeI 1086 TGGCCA [CT]^GGCC[GA] Eam1105I 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 35 GAAGAG CTCTTC(1/4) EcoO109I 99 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1228 GAATTC G^AATTC EcoRV 954 GATATC GAT^ATC FauI 676 GCGGG CCCGC(4/6) Fnu4HI 663 GCTGC GC^[ACGT]GC HgaI 1098 GACGC GACGC(5/10) HhaI 1060 GCGC GCG^C HinP1I 1060 GCGC G^CGC HindIII 1160 AAGCTT A^AGCTT ItaI 663 GCTGC GC^[ACGT]GC Ksp632I 35 GAAGAG CTCTTC(1/4) MaeI 982 CTAG C^TAG MamI 796 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 314 CAATTG C^AATTG MluNI 1086 TGGCCA TGG^CCA MscI 1086 TGGCCA TGG^CCA MunI 314 CAATTG C^AATTG PpuMI 99 AGGACCC [GA]G^G[AT]CC[CT] SexAI 932 ACCTGGT A^CC[AT]GGT SfaNI 886 GATGC GCATC(5/9) SmlI 706 CTTGAG C^T[CT][GA]AG StuI 687 AGGCCT AGG^CCT TfiI 1116 GATTC G^A[AT]TC TseI 663 GCTGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 675 CGCG CG^CG 1061 CGCG AccIII 882 TCCGGA T^CCGGA 1203 TCCGGA AseI 789 ATTAAT AT^TAAT 843 ATTAAT AsnI 789 ATTAAT AT^TAAT 843 ATTAAT Asp700I 602 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 863 GAATTTCTTC AvaI 130 CCCGAG C^[CT]CG[GA]G 1123 CCCGAG AvaII 100 GGACC G^G[AT]CC 280 GGACC BamHI 199 GGATCC G^GATCC 1200 GGATCC BanI 185 GGCACC G^G[CT][GA]CC 680 GGTGCC BbsI 1095 GAAGAC GAAGAC(2/6) 1439 GTCTTC BclI 525 TGATCA T^GATCA 1368 TGATCA BcnI 1396 CCGGG CC^[GC]GG 1495 CCGGG BlpI 375 GCTGAGC GC^T[ACGT]AGC 1141 GCTGAGC Bpu1102I 375 GCTGAGC GC^T[ACGT]AGC 1141 GCTGAGC BpuAI 1095 GAAGAC GAAGAC(2/6) 1439 GTCTTC BseAI 882 TCCGGA T^CCGGA 1203 TCCGGA BseRI 437 GAGGAG GAGGAG(10/8) 1126 GAGGAG BsmAI 334 GAGAC GTCTC(1/5) 1054 GAGAC BsoBI 130 CCCGAG C^[CT]CG[GA]G 1123 CCCGAG BspEI 882 TCCGGA T^CCGGA 1203 TCCGGA BstUI 675 CGCG CG^CG 1061 CGCG CelII 375 GCTGAGC GC^T[ACGT]AGC 1141 GCTGAGC Cfr13I 100 GGACC G^G[ACGT]CC 280 GGACC FbaI 525 TGATCA T^GATCA 1368 TGATCA HphI 427 GGTGA GGTGA(8/7) 1419 GGTGA MroI 882 TCCGGA T^CCGGA 1203 TCCGGA MvnI 675 CGCG CG^CG 1061 CGCG NciI 1396 CCGGG CC^[GC]GG 1495 CCGGG NspI 57 ACATGC [GA]CATG^[CT] 451 GCATGT PshBI 789 ATTAAT AT^TAAT 843 ATTAAT Sau96I 100 GGACC G^G[ACGT]CC 280 GGACC TaqI 33 TCGA T^CGA 1508 TCGA Tsp45I 5 GTGAC ^GT[GC]AC 1434 GTCAC TthHB8I 33 TCGA T^CGA 1508 TCGA XmnI 602 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 863 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BmyI BpmI BsaJI BsaWI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII FokI HaeIII HapII HinfI HpaII MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI ScrFI Tru9I Tsp509I TspRI XhoII