Restriction map for
pFN21AE4130
(Circular) (Six-base) MAPSORT of: pFN21AE4130.seq Check: 3899 from: 1 to: 6286
pFN21AE4130 6286 bp
With 182 enzymes: SgfI *
March 28, 2012 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2838 2838
Size: 6286
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3004 3629 1937
Size: 1067 625 4594
Fragments arranged by size:
4594 1067 625
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 2642 3119 3309 3368 4349 5733 6001
Size: 840 170 477 190 59 981 1384 268
Cuts at: 6001 1632
Size: 1917
Fragments arranged by size:
1917 1384 981 840 477 268 190 170
59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 3532 5015 3532
Size: 1483 4803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 2835
Size: 32 6254
AlwNI CAG_nnn'CTG
Cuts at: 4029 5601 4029
Size: 1572 4714
ApaLI G'TGCA_C
Cuts at: 3282 4343 5499 3282
Size: 1061 1156 4069
Fragments arranged by size:
4069 1156 1061
ApoI r'AATT_y
Cuts at: 1493 2230 2579 2868 3233 3595 3821 3973 6082
Size: 737 349 289 365 362 226 152 2109
Cuts at: 6082 1493
Size: 1697
Fragments arranged by size:
2109 1697 737 365 362 349 289 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2795 2849 4840 160
Size: 872 1763 54 1991 1606
Fragments arranged by size:
1991 1763 1606 872 54
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 3128 3611 1870
Size: 73 192 993 483 4545
Fragments arranged by size:
4545 993 483 192 73
BamHI G'GATC_C
Cuts at: 2204 3205 3616 3983 2204
Size: 1001 411 367 4507
Fragments arranged by size:
4507 1001 411 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2190 2685 3607 5062 618
Size: 325 217 627 403 495 922 1455 1842
Fragments arranged by size:
1842 1455 922 627 495 403 325 217
BanII G_rGCy'C
Cuts at: 1391 3407 3605 1391
Size: 2016 198 4072
Fragments arranged by size:
4072 2016 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3107 3437 928
Size: 971 1208 330 3777
Fragments arranged by size:
3777 1208 971 330
BbvCI CC'TCA_GC
Cuts at: 2661 2661
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 4477 4511 1903
Size: 34 1556 34 950 34 3678
Fragments arranged by size:
3678 1556 950 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 2530 3373 1548
Size: 982 843 4461
Fragments arranged by size:
4461 982 843
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2776 6281 2776
Size: 3505 2781
BlpI GC'TnA_GC
Cuts at: 2381 3147 3703 2381
Size: 766 556 4964
Fragments arranged by size:
4964 766 556
Bme1580I G_kGCm'C
Cuts at: 1790 2690 3286 4347 5503 1790
Size: 900 596 1061 1156 2573
Fragments arranged by size:
2573 1156 1061 900 596
BmgBI CAC'GTC
Cuts at: 1318 3443 1318
Size: 2125 4161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 2741 3407 4946 1239
Size: 1478 24 666 1539 2579
Fragments arranged by size:
2579 1539 1478 666 24
Bpu10I CC'TnA_GC
Cuts at: 2661 2661
Size: 6286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2732 4411 5276 5574 2732
Size: 1679 865 298 3444
Fragments arranged by size:
3444 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 2805 6280 2805
Size: 3475 2811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2588 2887 3208 4719 5117 5391 5538 5949 2588
Size: 299 321 1511 398 274 147 411 2925
Fragments arranged by size:
2925 1511 411 398 321 299 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2457 3146 1186
Size: 1271 689 4326
Fragments arranged by size:
4326 1271 689
BseYI C'CCAG_C
Cuts at: 5489 5985 5489
Size: 496 5790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2436 1163
Size: 573 700 5013
Fragments arranged by size:
5013 700 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3286 4347 4432 5503 3286
Size: 1061 85 1071 4069
Fragments arranged by size:
4069 1071 1061 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
BsmBI CGTCTCn'nnnn_
Cuts at: 3053 3053
Size: 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2690 3286 3407 3605 4347 4432 5503
Size: 399 900 596 121 198 742 85 1071
Cuts at: 5503 1391
Size: 2174
Fragments arranged by size:
2174 1071 900 742 596 399 198 121
85
BspEI T'CCGG_A
Cuts at: 2887 3208 5949 2887
Size: 321 2741 3224
Fragments arranged by size:
3224 2741 321
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3641 844
Size: 1994 803 3489
Fragments arranged by size:
3489 1994 803
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 4781 4955 65
Size: 1183 1275 2258 174 1396
Fragments arranged by size:
2258 1396 1275 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2204 2776 3205 3616 3983 4368 4385 5837
Size: 232 572 429 411 367 385 17 1452
Cuts at: 5837 5923 5935 6281 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 572 429 411 385 367 346
232 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 4702
Fragments arranged by size:
4702 1032 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3595 487
Size: 683 56 2369 3178
Fragments arranged by size:
3178 2369 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4596 4616 3802
Size: 794 20 5472
Fragments arranged by size:
5472 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 2182 3590 2182
Size: 1408 4878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 3733 4622
Size: 54 1425 50 275 18 1261 642 889
Cuts at: 4622 6251 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1261 889 642 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2035 4216 1353
Size: 682 2181 3423
Fragments arranged by size:
3423 2181 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3154 4873 5247 5393 387
Size: 1064 138 1565 1719 374 146 1280
Fragments arranged by size:
1719 1565 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 2642 2717 2741 3119 3309 3368
Size: 393 840 170 75 24 378 190 59
Cuts at: 3368 3407 4349 4946 5733 6001 1239
Size: 39 942 597 787 268 1524
Fragments arranged by size:
1524 942 840 787 597 393 378 268
190 170 75 59 39 24
EcoO109I rG'GnC_Cy
Cuts at: 2105 2105
Size: 6286
EcoRI G'AATT_C
Cuts at: 3233 3595 3973 3233
Size: 362 378 5546
Fragments arranged by size:
5546 378 362
EcoRV GAT'ATC
Cuts at: 2961 2961
Size: 6286
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2768 2800 3518 3550 4927
Size: 32 135 32 1196 32 718 32 1377
Cuts at: 4927 4959 5001 5033 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1377 1196 718 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3165 748
Size: 2417 3869
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 2319 3885 2319
Size: 1566 4720
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5400 5584 1305
Size: 4095 184 2007
Fragments arranged by size:
4095 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3093 10
Size: 54 1425 343 1261 3203
Fragments arranged by size:
3203 1425 1261 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4244 4603 4762 518
Size: 648 3078 359 159 2042
Fragments arranged by size:
3078 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2380 2812 3097 3702 4379 5527 5772
Size: 418 356 432 285 605 677 1148 245
Cuts at: 5772 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 605 432 418 356 285
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2066 2460 3644 5189 1132
Size: 97 837 394 1184 1545 2229
Fragments arranged by size:
2229 1545 1184 837 394 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PfoI T'CCnGG_A
Cuts at: 3384 3384
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2105 2105
Size: 6286
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2380 2812 3097 2380
Size: 432 285 5569
Fragments arranged by size:
5569 432 285
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SexAI A'CCwGG_T
Cuts at: 2937 2937
Size: 6286
SfcI C'TryA_G
Cuts at: 826 1045 3634 4769 5450 5641 826
Size: 219 2589 1135 681 191 1471
Fragments arranged by size:
2589 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 4390 5291 5553 820
Size: 197 926 768 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 768 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 2694 1517
Size: 1177 5109
StyI C'CwwG_G
Cuts at: 513 1065 3725 513
Size: 552 2660 3074
Fragments arranged by size:
3074 2660 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2892 4313 4330 4483 4668 5113 325
Size: 1255 1312 1421 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1312 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2617 4532 6244
Size: 274 80 33 51 1443 640 1915 1712
Cuts at: 6244 96
Size: 138
Fragments arranged by size:
1915 1712 1443 640 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3232 3264 6246 6278 3232
Size: 32 2982 32 3240
Fragments arranged by size:
3240 2982 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2611 2872 4415 2611
Size: 261 1543 4482
Fragments arranged by size:
4482 1543 261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI