(Linear) (Six-base) MAPSORT of: KIEE4130.seq Check: 6044 from: 1 to: 1581 KIEE4130.orf With 182 enzymes: SgfI * March 22, 2012 17:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 833 1581 Size: 833 748 AccI GT'mk_AC Cuts at: 0 999 1581 Size: 999 582 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 467 637 1114 1304 1363 1581 Size: 467 170 477 190 59 218 Fragments arranged by size: 477 467 218 190 170 59 AhdI GACnn_n'nnGTC Cuts at: 0 1527 1581 Size: 1527 54 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 830 862 1581 Size: 830 32 719 Fragments arranged by size: 830 719 32 ApaLI G'TGCA_C Cuts at: 0 1277 1581 Size: 1277 304 ApoI r'AATT_y Cuts at: 0 225 574 863 1228 1581 Size: 225 349 289 365 353 Fragments arranged by size: 365 353 349 289 225 AseI AT'TA_AT Cuts at: 0 790 844 1581 Size: 790 54 737 Fragments arranged by size: 790 737 54 AvaI C'yCGr_G Cuts at: 0 130 1123 1581 Size: 130 993 458 Fragments arranged by size: 993 458 130 BamHI G'GATC_C Cuts at: 0 199 1200 1581 Size: 199 1001 381 Fragments arranged by size: 1001 381 199 BanI G'GyrC_C Cuts at: 0 185 680 1581 Size: 185 495 901 Fragments arranged by size: 901 495 185 BanII G_rGCy'C Cuts at: 0 1402 1581 Size: 1402 179 BbsI GAAGACnn'nnnn_ Cuts at: 0 1102 1432 1581 Size: 1102 330 149 Fragments arranged by size: 1102 330 149 BbvCI CC'TCA_GC Cuts at: 0 656 1581 Size: 656 925 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1488 1522 1581 Size: 1488 34 59 Fragments arranged by size: 1488 59 34 BclI T'GATC_A Cuts at: 0 525 1368 1581 Size: 525 843 213 Fragments arranged by size: 843 525 213 BglII A'GATC_T Cuts at: 0 771 1581 Size: 771 810 BlpI GC'TnA_GC Cuts at: 0 376 1142 1581 Size: 376 766 439 Fragments arranged by size: 766 439 376 Bme1580I G_kGCm'C Cuts at: 0 685 1281 1581 Size: 685 596 300 Fragments arranged by size: 685 596 300 BmgBI CAC'GTC Cuts at: 0 1438 1581 Size: 1438 143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 736 1402 1581 Size: 712 24 666 179 Fragments arranged by size: 712 666 179 24 Bpu10I CC'TnA_GC Cuts at: 0 656 1581 Size: 656 925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 1581 Size: 727 854 BsaBI GATnn'nnATC Cuts at: 0 800 1581 Size: 800 781 BsaWI w'CCGG_w Cuts at: 0 583 882 1203 1581 Size: 583 299 321 378 Fragments arranged by size: 583 378 321 299 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 452 1141 1581 Size: 452 689 440 Fragments arranged by size: 689 452 440 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 1581 Size: 431 1150 BsiHKAI G_wGCw'C Cuts at: 0 1281 1581 Size: 1281 300 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1048 1581 Size: 1048 533 Bsp1286I G_dGCh'C Cuts at: 0 685 1281 1402 1581 Size: 685 596 121 179 Fragments arranged by size: 685 596 179 121 BspEI T'CCGG_A Cuts at: 0 882 1203 1581 Size: 882 321 378 Fragments arranged by size: 882 378 321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 833 1581 Size: 833 748 BsrDI GCAATG_nn' Cuts at: 0 518 1581 Size: 518 1063 BstYI r'GATC_y Cuts at: 0 199 771 1200 1581 Size: 199 572 429 381 Fragments arranged by size: 572 429 381 199 BtgI C'CryG_G Cuts at: 0 92 1581 Size: 92 1489 DraI TTT'AAA Cuts at: 0 177 1581 Size: 177 1404 EaeI y'GGCC_r Cuts at: 0 1086 1581 Size: 1086 495 EarI CTCTTCn'nnn_ Cuts at: 0 30 1581 Size: 30 1551 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1149 1581 Size: 1149 432 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 467 637 712 736 1114 1304 1363 1402 Size: 467 170 75 24 378 190 59 39 Cuts at: 1402 1581 Size: 179 Fragments arranged by size: 467 378 190 179 170 75 59 39 24 EcoO109I rG'GnC_Cy Cuts at: 0 100 1581 Size: 100 1481 EcoRI G'AATT_C Cuts at: 0 1228 1581 Size: 1228 353 EcoRV GAT'ATC Cuts at: 0 956 1581 Size: 956 625 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 763 795 1513 1545 1581 Size: 763 32 718 32 36 Fragments arranged by size: 763 718 36 32 32 HindIII A'AGCT_T Cuts at: 0 1160 1581 Size: 1160 421 MfeI C'AATT_G Cuts at: 0 314 1581 Size: 314 1267 MscI TGG'CCA Cuts at: 0 1088 1581 Size: 1088 493 MspA1I CmG'CkG Cuts at: 0 19 375 807 1092 1581 Size: 19 356 432 285 489 Fragments arranged by size: 489 432 356 285 19 NspI r_CATG'y Cuts at: 0 61 455 1581 Size: 61 394 1126 Fragments arranged by size: 1126 394 61 PfoI T'CCnGG_A Cuts at: 0 1379 1581 Size: 1379 202 PpuMI rG'GwC_Cy Cuts at: 0 100 1581 Size: 100 1481 PvuII CAG'CTG Cuts at: 0 375 807 1092 1581 Size: 375 432 285 489 Fragments arranged by size: 489 432 375 285 SexAI A'CCwGG_T Cuts at: 0 932 1581 Size: 932 649 SmlI C'TyrA_G Cuts at: 0 706 1581 Size: 706 875 StuI AGG'CCT Cuts at: 0 689 1581 Size: 689 892 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 887 1581 Size: 887 694 TatI w'GTAC_w Cuts at: 0 612 1581 Size: 612 969 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1227 1259 1581 Size: 1227 32 322 Fragments arranged by size: 1227 322 32 XmnI GAAnn'nnTTC Cuts at: 0 606 867 1581 Size: 606 261 714 Fragments arranged by size: 714 606 261 Enzymes that do cut: AarI AccI AcuI AhdI AloI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaWI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BstYI BtgI DraI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII MfeI MscI MspA1I NspI PfoI PpuMI PvuII SexAI SmlI StuI TaqII TatI TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaI AscI AsiSI AvrII BaeI BbeI BciVI BfrBI BglI BmrI BmtI BplI BsaI BsaAI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI