################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeI MflI MluI MroI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 729 GTATAC GT^[AC][GT]AC AciI 597 GCGG CCGC(-3/-1) AcsI 797 AAATTC [GA]^AATT[CT] AfaI 527 GTAC GT^AC AhdI 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 16 GGATC GGATC(4/5) ApoI 797 AAATTC [GA]^AATT[CT] AspEI 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 236 GTGCTC G[AT]GC[AT]^C AvaII 703 GGACC G^G[AT]CC BalI 749 TGGCCA TGG^CCA BbsI 469 GAAGAC GAAGAC(2/6) BmyI 236 GTGCTC G[AGT]GC[ACT]^C BpmI 584 CTGGAG CTGGAG(16/14) BpuAI 469 GAAGAC GAAGAC(2/6) BsaBI 334 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 236 GTGCTC G[AT]GC[AT]^C BsmFI 626 GGGAC GGGAC(10/14) BsmI 762 GAATGC GAATGC(1/-1) Bsp1286I 236 GTGCTC G[AGT]GC[ACT]^C BssKI 306 CCAGG ^CC[ACGT]GG Bst1107I 729 GTATAC GTA^TAC BstNI 306 CCAGG CC^[AT]GG BstZ17I 729 GTATAC GTA^TAC Cfr13I 703 GGACC G^G[ACGT]CC DraI 115 TTTAAA TTT^AAA EaeI 749 TGGCCA [CT]^GGCC[GA] Eam1105I 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 306 CCAGG ^CC[AT]GG EcoT22I 337 ATGCAT ATGCA^T HphI 97 TCACC GGTGA(8/7) MaeII 663 ACGT A^CGT MaeIII 544 GTCAC ^GT[ACGT]AC MamI 334 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 451 CAATTG C^AATTG MluNI 749 TGGCCA TGG^CCA MscI 749 TGGCCA TGG^CCA MunI 451 CAATTG C^AATTG MvaI 306 CCAGG CC^[AT]GG MwoI 766 GCTTGTTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 808 CATATG CA^TATG NsiI 337 ATGCAT ATGCA^T PleI 542 GAGTC GAGTC(4/5) RsaI 527 GTAC GT^AC Sau96I 703 GGACC G^G[ACGT]CC ScrFI 306 CCAGG CC^[ACGT]GG TfiI 265 GAATC G^A[AT]TC Tsp45I 544 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AseI 423 ATTAAT AT^TAAT 442 ATTAAT AsnI 423 ATTAAT AT^TAAT 442 ATTAAT BseRI 217 CTCCTC GAGGAG(10/8) 498 CTCCTC EarI 68 GAAGAG CTCTTC(1/4) 856 GAAGAG HaeIII 111 GGCC GG^CC 750 GGCC HinfI 265 GAATC G^A[ACGT]TC 542 GAGTC Ksp632I 68 GAAGAG CTCTTC(1/4) 856 GAAGAG NlaIII 43 CATG CATG^ 148 CATG PshBI 423 ATTAAT AT^TAAT 442 ATTAAT SfcI 362 CTACAG C^T[GA][CT]AG 370 CTACAG TaqI 351 TCGA T^CGA 554 TCGA TspRI 612 CAGTG CA[GC]TG[ACGT][ACGT]^ 699 CACTG TthHB8I 351 TCGA T^CGA 554 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BsmAI BsrI BstF5I DdeI DpnII FokI MboI MboII MnlI MseI NdeII Sau3AI SfaNI Tru9I Tsp509I