################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DdeI DpnII DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MspA1I MunI NaeI NarI NdeI NdeII NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 122 ACGTGT A^C[GA][CT]GT Alw44I 145 GTGCAC G^TGCAC Aor51HI 257 AGCGCT AGC^GCT ApaLI 145 GTGCAC G^TGCAC Asp700I 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 145 GTGCAC G[AT]GC[AT]^C BalI 320 TGGCCA TGG^CCA BanI 343 GGCACC G^G[CT][GA]CC BcnI 310 CCCGG CC^[GC]GG BglI 223 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 121 TACGTG [CT]AC^GT[GA] BsgI 283 GTGCAG GTGCAG(16/14) BsiHKAI 145 GTGCAC G[AT]GC[AT]^C BsmFI 273 GTCCC GGGAC(10/14) Bsp1407I 125 TGTACA T^GTACA BspHI 185 TCATGA T^CATGA BspMI 141 GCAGGT ACCTGC(4/8) BsrFI 157 ACCGGC [GA]^CCGG[CT] BsrGI 125 TGTACA T^GTACA BstNI 240 CCTGG CC^[AT]GG BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 157 ACCGGC [GA]^CCGG[CT] DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG Eco47III 257 AGCGCT AGC^GCT EcoRII 240 CCTGG ^CC[AT]GG HaeII 257 AGCGCT [GA]GCGC^[CT] MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MvaI 240 CCTGG CC^[AT]GG NciI 310 CCCGG CC^[GC]GG NcoI 176 CCATGG C^CATGG NotI 95 GCGGCCGC GC^GGCCGC RcaI 185 TCATGA T^CATGA SspBI 125 TGTACA T^GTACA StuI 230 AGGCCT AGG^CCT TaqI 212 TCGA T^CGA TthHB8I 212 TCGA T^CGA XmnI 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 94 CGCG CG^CG 277 CGCG AfaI 126 GTAC GT^AC 363 GTAC BbvI 303 GCTGC GCAGC(8/12) 306 GCTGC BmyI 145 GTGCAC G[AGT]GC[ACT]^C 342 GGGCAC BsiEI 96 CGGCCG CG[GA][CT]^CG 290 CGGCCG BsiYI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGACACCGG BslI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGACACCGG Bsp1286I 145 GTGCAC G[AGT]GC[ACT]^C 342 GGGCAC BssKI 240 CCTGG ^CC[ACGT]GG 310 CCCGG BstUI 94 CGCG CG^CG 277 CGCG EagI 96 CGGCCG C^GGCCG 290 CGGCCG EclXI 96 CGGCCG C^GGCCG 290 CGGCCG Eco52I 96 CGGCCG C^GGCCG 290 CGGCCG FauI 236 CCCGC CCCGC(4/6) 275 CCCGC HapII 158 CCGG C^CGG 311 CCGG HpaII 158 CCGG C^CGG 311 CCGG HphI 56 TCACC GGTGA(8/7) 356 TCACC MaeII 122 ACGT A^CGT 271 ACGT MaeIII 55 GTCAC ^GT[ACGT]AC 355 GTCAC MboII 69 GAAGA GAAGA(8/7) 209 TCTTC MspI 158 CCGG C^CGG 311 CCGG MvnI 94 CGCG CG^CG 277 CGCG NlaIII 177 CATG CATG^ 186 CATG NlaIV 21 GGCTCC GG[ACGT]^[ACGT]CC 343 GGCACC RsaI 126 GTAC GT^AC 363 GTAC ScrFI 240 CCTGG CC^[ACGT]GG 310 CCCGG TseI 303 GCTGC G^C[AT]GC 306 GCTGC Tsp45I 55 GTCAC ^GT[GC]AC 355 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI AluI BsaJI Cac8I CfoI EaeI EcoT14I Fnu4HI HaeIII HhaI HinP1I ItaI MnlI MwoI SfaNI StyI