Restriction map for
pFN21AE6370
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MflI MluI MroI MseI
MslI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 754 GAATTC [GA]^AATT[CT]
AcyI 187 GGCGTC G[GA]^CG[CT]C
Alw44I 229 GTGCAC G^TGCAC
AlwNI 902 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 357 CCTCAGG CC^T[ACGT]AGG
ApaLI 229 GTGCAC G^TGCAC
ApoI 754 GAATTC [GA]^AATT[CT]
AvaII 99 GGTCC G^G[AT]CC
AviII 294 TGCGCA TGC^GCA
BalI 950 TGGCCA TGG^CCA
BanI 638 GGTGCC G^G[CT][GA]CC
BbsI 468 GAAGAC GAAGAC(2/6)
BfaI 568 CTAG C^TAG
BglI 952 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 895 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 895 GCTCAGC GC^T[ACGT]AGC
BpuAI 468 GAAGAC GAAGAC(2/6)
BsaAI 340 TACGTA [CT]AC^GT[GA]
BsaHI 187 GGCGTC G[GA]^CG[CT]C
BsaI 586 GAGACC GGTCTC(1/5)
BsmI 705 GAATGC GAATGC(1/-1)
BstAPI 603 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 357 CCTCAGG CC^T[ACGT]AGG
CelII 895 GCTCAGC GC^T[ACGT]AGC
DraIII 378 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 214 CCGTGG C^C[GA][CT]GG
Eco81I 357 CCTCAGG CC^T[ACGT]AGG
EcoRI 754 GAATTC G^AATTC
FspI 294 TGCGCA TGC^GCA
Hin1I 187 GGCGTC G[GA]^CG[CT]C
MaeI 568 CTAG C^TAG
MaeIII 446 GTGAC ^GT[ACGT]AC
MboII 468 GAAGA GAAGA(8/7)
MfeI 543 CAATTG C^AATTG
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
MunI 543 CAATTG C^AATTG
NdeI 419 CATATG CA^TATG
PflMI 542 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 656 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 601 CTGCAG CTGCA^G
ScaI 275 AGTACT AGT^ACT
SfcI 601 CTGCAG C^T[GA][CT]AG
SmaI 83 CCCGGG CCC^GGG
SnaBI 340 TACGTA TAC^GTA
SpeI 567 ACTAGT A^CTAGT
TfiI 125 GAATC G^A[AT]TC
Tsp45I 446 GTGAC ^GT[GC]AC
Van91I 542 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 83 CCCGGG C^CCGGG
XmaI 83 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 335 ACATGT A^C[GA][CT]GT
659 ACATGT
AlwI 405 GATCC GGATC(4/5)
624 GATCC
AspHI 229 GTGCAC G[AT]GC[AT]^C
496 GTGCTC
BsiHKAI 229 GTGCAC G[AT]GC[AT]^C
496 GTGCTC
BsmAI 586 GAGAC GTCTC(1/5)
571 GTCTC
BspLU11I 335 ACATGT A^CATGT
659 ACATGT
BspMI 644 ACCTGC ACCTGC(4/8)
219 GCAGGT
CfoI 196 GCGC GCG^C
295 GCGC
DpnII 405 GATC ^GATC
624 GATC
EaeI 211 TGGCCG [CT]^GGCC[GA]
950 TGGCCA
FauI 473 CCCGC CCCGC(4/6)
618 CCCGC
HgaI 188 GCGTC GACGC(5/10)
941 GCGTC
HhaI 196 GCGC GCG^C
295 GCGC
HinP1I 196 GCGC G^CGC
295 GCGC
MboI 405 GATC ^GATC
624 GATC
NdeII 405 GATC ^GATC
624 GATC
PleI 730 GACTC GAGTC(4/5)
490 GAGTC
Sau3AI 405 GATC ^GATC
624 GATC
StuI 240 AGGCCT AGG^CCT
345 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PvuII RsaI Sau96I ScrFI TseI
Tsp509I TspRI